scholarly journals Exploring the role of extracellular matrix proteins to develop biomarkers of plaque vulnerability and outcome

2020 ◽  
Vol 287 (5) ◽  
pp. 493-513 ◽  
Author(s):  
S. Holm Nielsen ◽  
L. Jonasson ◽  
K. Kalogeropoulos ◽  
M. A. Karsdal ◽  
A. L. Reese‐Petersen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Plaque Vulnerability

Role of high molecular weight extracellular matrix proteins in glioma cell migration

1997 ◽  
Vol 23 (2) ◽  
pp. 102-112 ◽  
Author(s):  
R. Mahesparan ◽  
B. B. Tysnes ◽  
K. Edvardsen ◽  
H. K. Haugeland ◽  
I. Garcia Cabrera ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Extracellular Matrix ◽  
Cell Migration ◽  
High Molecular Weight ◽  
Glioma Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Glioma Cell Migration

Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins

2015 ◽  
Vol 63 (5) ◽  
pp. 377-384 ◽  
Author(s):  
Marta Pokrywczynska ◽  
Marzena Anna Lewandowska ◽  
Sandra Krzyzanowska ◽  
Arkadiusz Jundzill ◽  
Marta Rasmus ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Marrow Mesenchymal Stem Cells

scholarly journals High-Density Lipoproteins Limit Neutrophil-Induced Damage to the Blood–Brain Barrier in Vitro

2013 ◽  
Vol 33 (4) ◽  
pp. 575-582 ◽  
Author(s):  
Quoc Bao Dang ◽  
Bertrand Lapergue ◽  
Alexy Tran-Dinh ◽  
Devy Diallo ◽  
Juan-Antonio Moreno ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Blood Brain Barrier ◽  
Extracellular Matrix Proteins ◽  
High Density ◽  
Matrix Proteins ◽  
Brain Barrier ◽  
High Density Lipoproteins ◽  
Blood Brain

Breakdown of the blood–brain barrier (BBB) is a key step associated with ischemic stroke and its increased permeability causes extravasation of plasma proteins and circulating leukocytes. Polymorphonuclear neutrophil (PMN) proteases may participate in BBB breakdown. We investigated the role of PMNs in ischemic conditions by testing their effects on a model of BBB in vitro, under oxygen-glucose deprivation (OGD) to mimic ischemia, supplemented or not with high-density lipoproteins (HDLs) to assess their potential protective effects. Human cerebral endothelial cells cultured on transwells were incubated for 4 hours under OGD conditions with or without PMNs and supplemented or not with HDLs or alpha-1 antitrypsin (AAT, an elastase inhibitor). The integrity of the BBB was then assessed and the effect of HDLs on PMN-induced proteolysis of extracellular matrix proteins was evaluated. The release of myeloperoxidase and matrix metalloproteinase 9 (MMP-9) by PMNs was quantified. Polymorphonuclear neutrophils significantly increased BBB permeability under OGD conditions via proteolysis of extracellular matrix proteins. This was associated with PMN degranulation. Addition of HDLs or AAT limited the proteolysis and associated increased permeability by inhibiting PMN activation. Our results suggest a deleterious, elastase-mediated role of activated PMNs under OGD conditions leading to BBB disruption that could be inhibited by HDLs.

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