Comparison of term and preterm labor procalcitonin and leukocyte cell volume, conductivity and light scatter (VCS) parameters in order to demonstrate the impact of inflammation on the triggering mechanisms of preterm uterin contractions

2020 ◽  
Vol 46 (5) ◽  
pp. 694-698
Author(s):  
Canan Unal ◽  
Esra Karatas ◽  
Erdem Fadıloglu ◽  
Oytun Portakal ◽  
Mehmet Sinan Beksac
Keyword(s):  
Cell Volume ◽  
Preterm Labor ◽  
Light Scatter ◽  
Volume Conductivity ◽  
Triggering Mechanisms ◽  
The Impact

scholarly journals Contamination-resistant, rapid emulsion-based isothermal nucleic acid amplification with Mie-scatter inspired light scatter analysis for bacterial identification

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexander S. Day ◽  
Tiffany-Heather Ulep ◽  
Elizabeth Budiman ◽  
Laurel Dieckhaus ◽  
Babak Safavinia ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Amplification ◽  
Nucleic Acid Detection ◽  
Light Scatter ◽  
Emulsion Droplets ◽  
Assay Performance ◽  
Bacterial Adsorption ◽  
Scatter Intensity ◽  
The Impact ◽  
Theory Light

AbstractAn emulsion loop-mediated isothermal amplification (eLAMP) platform was developed to reduce the impact that contamination has on assay performance. Ongoing LAMP reactions within the emulsion droplets cause a decrease in interfacial tension, causing a decrease in droplet size, which results in decreased light scatter intensity due to Mie theory. Light scatter intensity was monitored via spectrophotometers and fiber optic cables placed at 30° and 60°. Light scatter intensities collected at 3 min, 30° were able to statistically differentiate 103 and 106 CFU/µL initial Escherichia coli O157:H7 concentrations compared to NTC (0 CFU/µL), while the intensity at 60° were able to statistically differentiate 106 CFU/µL initial concentrations and NTC. Control experiments were conducted to validate nucleic acid detection versus bacterial adsorption, finding that the light scatter intensities change is due specifically to ongoing LAMP amplification. After inducing contamination of bulk LAMP reagents, specificity lowered to 0% with conventional LAMP, while the eLAMP platform showed 87.5% specificity. We have demonstrated the use of angle-dependent light scatter intensity as a means of real-time monitoring of an emulsion LAMP platform and fabricated a smartphone-based monitoring system that showed similar trends as spectrophotometer light scatter data, validating the technology for a field deployable platform.

scholarly journals Impact of parity and gestational age of mothers with hypertension on birth weight, red blood cells and mode of delivery of their babies

1970 ◽  
Vol 19 (4) ◽  
pp. 3038-3044
Author(s):  
Helen Chioma Okoye ◽  
Chilota Chibuife Efobi ◽  
Josephat Maduabuchi Chinawa ◽  
Odutola Israel Odetunde ◽  
Awoere Tamunosiki Chinawa ◽  
...  
Keyword(s):  
Birth Weight ◽  
Red Blood Cells ◽  
Cell Volume ◽  
Gestational Age ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Mode Of Delivery ◽  
Poor Correlation ◽  
Hypertensive Group ◽  
The Impact

Background: Maternal factors are determinants of birth outcome which includes birth weight, haematological indices and mode of delivery of their babies.Objectives: To determine the impact of parity and gestational age of hypertensive mothers on some neonatal variables.Methods: A hospital based cross-sectional study of measurement of neonatal variables (birth weight, red blood cells and mode of delivery) among hypertensive mothers and their controls was conducted over a period of six months. Data were analyzed using the Statistical Package for Social Sciences program (SPSS), version 20.Results: There were statistically significant differences in means between the neonates of the hypertensive group and non-hypertensive group for maternal age (t =1.61, p = 0.002), baby weight (t =2.87, p < 0.001), haemoglobin (Hb) (t =4.65, p = 0.010) and packed cell volume (PCV) (t =4.75, p = 0.009), but none for gravidity (t =1.95, p = 0.927)For all subjects, there was poor correlation between gestational age and variables; birth weight , haemoglobin (Hb), packed cell volume (PCV), nucleated red blood cell (nRBC) and parity. Likewise, parity poorly correlated with variables; age, birth weight, Hb, PCV, and nRBC. There was a statistically significant association between mode of delivery and hypertension (χ2 =53.082, p <0.001) but none with having a family history of hypertension (χ2 =1.13, p = 0.287).Conclusion: Parity and gestational age of mothers with hypertension have no impact on birth weight and red cells when compared with their non-hypertensive counterparts. However, mothers of babies delivered by elective and emergency caesarean section were about 2-3 times more likely to be hypertensive than those that delivered through spontaneous vertex delivery.Keywords: Hypertension; neonate; gestational age; parity.

scholarly journals Electrolytic degradation of DNA fluorochromes during flow cytometric measurement of electronic cell volume.

1980 ◽  
Vol 28 (4) ◽  
pp. 330-334 ◽  
Author(s):  
O Alabaster ◽  
D L Glaubiger ◽  
V T Hamilton ◽  
S A Bentley ◽  
S E Shackney ◽  
...  
Keyword(s):  
Cell Volume ◽  
Dna Content ◽  
Chemical Degradation ◽  
Quantum Yields ◽  
Light Scatter ◽  
Electrolytic Production ◽  
Flow Cytometric ◽  
Chlorine Gas ◽  
Electrode Polarity ◽  
Flow Cytometric Measurement

Changes in flow cytometric measurement of DNA content can result from electrolytic chemical degradation of mithramycin, ethidium bromide, and propidium iodide during simultaneous measurement of electronic cell volume. Bench electrolysis also degrades these fluorochromes without changing the quantum yields, even when they are complexed to DNA. In the flow cytometer, electrolytic production of chlorine at the anode is the probable cause of this degradation, since exposure of these fluorochromes to chlorine gas produces the same effect. It is therefore advisable to measure the DNA content distribution alone before simultaneously measuring the DNA content and the electronic cell volume. If unavoidable effects on the DNA distribution are present, narrow forward-angle light scatter should be used as the cell size indicator during dual parameter measurements. Modifying instrument design by reversing electrode polarity might eliminate this problem.

scholarly journals Pulmonary Tuberculosis in a Young Pregnant Female: Challenges in Diagnosis and Management

2008 ◽  
Vol 2008 ◽  
pp. 1-5 ◽  
Author(s):  
Manogna Maddineni ◽  
Mukta Panda
Keyword(s):  
Preterm Labor ◽  
Diagnosis And Treatment ◽  
Global Village ◽  
Disease Epidemiology ◽  
Infectious Disease Epidemiology ◽  
Acid Fast Bacilli ◽  
Disease Spectrum ◽  
Chest X Ray ◽  
High Index Of Suspicion ◽  
The Impact

Background. With the world becoming a global village, tuberculosis is no longer limited to endemic areas. Our case emphasizes the impact of immigration on infectious disease epidemiology and challenges associated with diagnosis and treatment in pregnancy.Case. A 21-year-old Hispanic female presented in preterm labor and was found to be hypoxic. Chest X-ray revealed a paratracheal mass which a CT scan confirmed. PPD test was positive. Bronchoalveolar lavage did not reveal acid-fast bacilli and biopsy revealed caseating granulomas. Diagnosis and treatment were challenging due to constraints in radiological investigations, lack of initial evidence of acid-fast bacilli, and toxic profile of medications. Due to her high risk, she was started on antituberculosis regimen. The diagnosis was confirmed on Day 26 whenMycobacterium tuberculosiswas isolated by DNA probe.Conclusion. A high index of suspicion is required to recognize the changing face and disease spectrum of tuberculosis and initiate treatment for better outcomes.

Rationalizing blood transfusion in cardiac surgery: the impact of a red cell volume-based guideline on blood usage and clinical outcome

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (3) ◽  
pp. 205-210 ◽  
Author(s):  
R. D. Slight ◽  
P. O'Donohoe ◽  
A. K. Y. Fung ◽  
C. Alonzi ◽  
D. B. L. McClelland ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Blood Transfusion ◽  
Clinical Outcome ◽  
Cell Volume ◽  
Red Cell ◽  
Red Cell Volume ◽  
Blood Usage ◽  
The Impact

scholarly journals Container Volume and Height Affect Shoot and Root Development in Marigold Seedlings

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 868B-868
Author(s):  
Jennifer Marohnic ◽  
Robert L. Geneve
Keyword(s):  
Cell Volume ◽  
Leaf Area ◽  
Seedling Growth ◽  
Root Development ◽  
Dry Weight ◽  
Seedling Development ◽  
Root Number ◽  
Positive Correlation ◽  
Root Dry Weight ◽  
The Impact

Marigold seedlings were grown in four containers that differed in both volume and shape. Seedlings grown in 1.5-gal containers showed the greatest potential for shoot and root development 20 days after sowing. These seedlings had greater leaf area, shoot and root dry weight, and total root number and length compared to seedlings grown in 406 plug trays, 72-cell packs, or 6-inch containers. There was a positive correlation (r2 = 0.81) between cell volume and seedling growth as well as a positive correlation (r2 = 0.89) between container height with seedling growth. An attempt was made to separate the impact of container volume vs. container height on seedling growth. Containers were designed using acrylics to vary the container height while keeping the volume constant at 1500 cm3. There was a positive correlation (r2 = 0.87) between shoot and root dry weight with container height. The data suggest that both container volume and height contribute to overall seedling growth in marigold, but when container volume is not limiting, container height has a large impact on seedling development.

scholarly journals Reticulocyte quantification by Coulter MAXM VCS (volume, conductivity, light scatter) technology

1996 ◽  
Vol 42 (12) ◽  
pp. 1930-1937 ◽  
Author(s):  
M Buttarello ◽  
P Bulian ◽  
M D Prà ◽  
P Barbera ◽  
P Rizzotti
Keyword(s):  
Reference Intervals ◽  
Light Scatter ◽  
Microscopic Method ◽  
Volume Conductivity ◽  
Low Concentrations

Abstract In this study the ability of the Coulter MAXM analyzer to quantify reticulocytes was evaluated. The results were compared with those obtained by a microscopic method according to NCCLS H44-P recommendations and with the results from the automated analyzer Sysmex R-1000. Duplicate samples from 330 patients were analyzed. The reference intervals obtained with the three methods were: MAXM 0.37-1.80%, median 0.83%; manual 0.40-2.30%, median 1.00%; R-1000 0.60-1.95%, median 1.06%. The imprecision (CV) at all concentrations is lower than the microscopic method (low 16.1% vs 67%; normal 16.9% vs 28.9%; high 9.5% vs 13.0%). The MAXM shows a good overall correlation with the microscopic method (intercept 0.01, slope 0.89, r2 = 0.87) despite evidence of a significant overestimation at low concentrations [difference (D) 0.30] and a moderate underestimation at normal (D -0.15) and high (D -1.04) concentrations; the same behavior is shown in comparison with results from the R-1000, with an overall underestimation by MAXM (D -0.26). When compared with the microscopic method, MAXM shows a modest sensitivity at low reticulocyte counts (54.8%) and satisfactory sensitivity for high counts (87.3%), with an overall agreement of 81.3%.

The impact of iron deficiency on blood cell volume

2019 ◽  
Vol 85 ◽  
pp. S63
Author(s):  
M. Yorguc ◽  
U. Kısa ◽  
E. Yorguc ◽  
M. Albayrak
Keyword(s):  
Iron Deficiency ◽  
Blood Cell ◽  
Cell Volume ◽  
The Impact
Sign in / Sign up

Export Citation Format

Share Document