Glabellar wrinkle correction by autologous fat transplantation and evidence of human adipose tissue regeneration in a nude mouse model

2021 ◽  
Author(s):  
Guangpeng Liu ◽  
Kaili Zhang ◽  
Yu Shi ◽  
Yongxian Lai
Keyword(s):  
Adipose Tissue ◽  
Mouse Model ◽  
Nude Mouse ◽  
Tissue Regeneration ◽  
Human Adipose Tissue ◽  
Fat Transplantation ◽  
Nude Mouse Model ◽  
Autologous Fat ◽  
Autologous Fat Transplantation

scholarly journals Improvement of the Survival of Human Autologous Fat Transplantation by Adipose-Derived Stem-Cells-Assisted Lipotransfer Combined with bFGF

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Aimei Jiang ◽  
Ming Li ◽  
Wenjing Duan ◽  
Yilong Dong ◽  
Yanmei Wang
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Histological Analysis ◽  
Angiogenic Factors ◽  
Fat Graft ◽  
Adipose Derived Stem Cells ◽  
Fat Transplantation ◽  
Autologous Fat ◽  
Fat Grafts ◽  
Autologous Fat Transplantation

Adipose-derived stem cells (ASCs) transplanted along with autologous adipose tissue may improve fat graft survival; however, the efficacy of ASCs has been diluted by low vascularization. This study was designed to test the hypothesis that basic fibroblast growth factor (bFGF) may improve the effects of ASCs because it owns the property to boost angiogenesis. In the present study, human fat tissues were mixed with ASCs, ASCs plus 100 U bFGF, or medium as the control and then injected subcutaneously into immunologically compromised nude mice for 12 weeks. Our findings demonstrated that mixture with the ASCs significantly increased the weight and volume of the fat grafts compared to control grafts, and histological analysis revealed that both ASCs and ASCs plus bFGF grafts consisted predominantly of adipose tissue and had significantly less fibrosis but greater microvascular density compared with control and also grafts mixed with ASCs had a high expression of angiogenic factors. More importantly, the bFGF treated fat grafts shown elevate in survival, vascularization, and angiogenic factors expression when compared with the grafts that received ASCs alone. These results indicated that bFGF together with ASCs can enhance the efficacy of autologous fat transplantation and increase blood vessel generation involved in the benefits from bFGF.

Autologous adipose tissue (lipofilling) in treatment of linear scleroderma (literature review)

2020 ◽  
pp. 15-17
Author(s):  
A. R. Misbakhova ◽  
N. E. Manturova ◽  
N. N. Murashkin ◽  
A. G. Stenko
Keyword(s):  
Adipose Tissue ◽  
Fat Grafting ◽  
Scientific Interest ◽  
Fat Transplantation ◽  
Medical Specialties ◽  
Autologous Fat ◽  
Autologous Fat Transplantation ◽  
Biological Substance ◽  
Day By Day ◽  
Indications For Use

Autologous fat is a biological substance that attracting increased scientific interest. Autologous fat considered as ideal filler due to its biocompatibility without risk of an allergic reaction or rejection. Likewise, this substance could be obtained easily, and costs are relatively low. Therapeutic indications for use fat grafting appear day by day, as it is recognized as an effective, reliable methodology and enhancing of areas and pathologies of application in medical specialties. The analysis results show that autologous fat transplantation gives a possibility to compensate for aesthetic and functional signs caused by facial scleroderma.

A Nude Mouse Model as an in vivo Infectivity Assay for Cryptospomdiosis

1993 ◽  
Vol 27 (3-4) ◽  
pp. 65-68 ◽  
Author(s):  
B. H. Kwa ◽  
M. Moyad ◽  
M. A. Pentella ◽  
J. B. Rose
Keyword(s):  
Drinking Water ◽  
Mouse Model ◽  
Nude Mouse ◽  
Cryptosporidium Parvum ◽  
Nude Mice ◽  
Surface Waters ◽  
Nude Mouse Model ◽  
Infectivity Assay ◽  
Limiting Dilution

Cryptosporidium parvum is an important patliogen of diarrlieal disease which has been implicated in several outbreaks associated with contamination of surface waters. In monitoring for C. parvum in drinking water sources, it is important to asce tain the viability, and more importantly, the infectivity of low numbers of recovered oocysts. Groups of 10 Balb/C nude (nu/nu) mice, 4-8 weeks old at time of inoculation, were infected with C. parvum oocysts from naturally infected calves and purified using Sheather's sucrose gradients. Oocysts were counted using the Merifluor IFA kit (Meridian). Each group of 10 mice were infected with 1,10,100 and 1000 oocysts respectively. Numbers of oocysts per inoculation were determined by limiting dilution, and parallel inocula were counted microscopically to ascertain the accuracy of the dilutions. Two uninfected nude mice were kept in each cage to serve as controls. Mouse stools were collected every 4 days, concentrated using the Fekal Kontrate Concentration Kit (Meridian) and oocysts were counted with a UV microscope using the Merifluor IFA Kit (Meridian). Oocyst counts were expressed in terms of number of oocyst/g feces. Mice inoculated with 1000 oocysts began to shed oocysts on day 32, mice inoculated with 100 oocysts began to shed on days 44-48, mice inoculated with 10 oocysts began to shed on days 56-60, and mice inoculated with 1 oocyst shed on days 68-88. All infected mice continued to shed oocysts intermittently and with variable oocyst counts until day 180 when the experiment was terminated. This study established that it is possible to infect nude mice with very low numbers, down to a single oocyst. We are currently in the process of correlating the nude mouse assay with other viability assays.

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