scholarly journals Genetic diversity of Klebsiella pneumoniae isolates during an outbreak in a non-human primate research colony

2016 ◽  
Vol 45 (6) ◽  
pp. 312-317 ◽  
Author(s):  
Alfonso S. Gozalo ◽  
William R. Elkins ◽  
Lynn E. Lambert ◽  
Frida Stock ◽  
Marvin L. Thomas ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Klebsiella Pneumoniae ◽  
Primate Research ◽  
Human Primate

Genetic diversity and molecular evolution of human and non-human primate Gammaherpesvirinae

2010 ◽  
Vol 10 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Vincent Lacoste ◽  
Anne Lavergne ◽  
Benoît de Thoisy ◽  
Jean-François Pouliquen ◽  
Antoine Gessain
Keyword(s):  
Genetic Diversity ◽  
Molecular Evolution ◽  
Human Primate

Seroepidemiology, Antimicrobial Susceptibility and Virulence Characteristics of Clinical Klebsiella pneumoniae Isolates in Mansoura University Hospitals

2021 ◽  
Vol 30 (1) ◽  
pp. 9-18
Author(s):  
Aya H. Elasmer ◽  
Mohammed Y. Ibrahim ◽  
Dina E. Rizk
Keyword(s):  
Genetic Diversity ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Genetic Relatedness ◽  
Diffusion Method ◽  
Serum Resistance ◽  
Beta Lactam ◽  
University Hospitals ◽  
Disk Diffusion Method ◽  
Pathogenic Potential

Background: Klebsiella pneumoniae is one of the crucial causes of nosocomial and community-acquired infections that can result in various infections in human. Objectives: The present study aim to investigate the prevalence of capsular serotypes, antimicrobial susceptibility and virulence characteristics of K. pneumoniae isolated from different Mansoura University Hospitals. Methodology: K. pneumoniae isolates were collected from different clinical sources at Mansoura University Hospitals. The antimicrobial susceptibility to 14 different antibiotics was determined by disk diffusion method. The capsular serotypes were assessed by quelling test. Serum resistance, haemagglutination, biofilm, lipase, protease and lecithinase enzymes production were assessed phenotypically. Moreover, four virulence genes (rmpA, fimH, kfuBC and wabG) were detected by PCR. The genetic relatedness among isolates was investigated using ERIC-PCR molecular typing. Results: Seventy-three isolates were confirmed as K. pneumoniae. The vast majority of isolates demonstrated MDR patterns (72.6%) including a high resistance rate to the beta-lactam antibiotics (ampicillin: 98.6%, amoxicillin-clavulanic acid: 97.26 %, piperacillin: 97.26 %, amoxicillin: 93.15% and cefotaxime: 94.52%). K1 and K2 were the main serotypes found among the isolates, K1 serotype was the predominant (79.45%). It was found that serum resistance was the highest detected virulence factor among isolates (95.9%) and lipase was the lowest detected factor (19.2%). Haemagglutination was detected in 63% of the isolates especially from rectal swab (83.3%) and sputum (72.7%). The biofilm formation was detected mainly among urine and blood isolates. rmpA, fimH, kfuBC and wabG genes were harbored by 20.5%, 92%, 66% and 94.5% of isolates, respectively. ERIC- PCR showed high genetic diversity (100%, typability, Simpson’s index of diversity= 1). Conclusion: The current study revealed the high antibiotic resistance levels, pathogenic potential, and genetic diversity among K. pneumoniae isolated from different clinical sources which is considered a serious health problem that necessitates interventions to control its spread.

scholarly journals Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations

2018 ◽  
Vol 4 (9) ◽  
Author(s):  
Margaret M. C. Lam ◽  
Ryan R. Wick ◽  
Kelly L. Wyres ◽  
Claire L. Gorrie ◽  
Louise M. Judd ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Klebsiella Pneumoniae ◽  
Mobile Element
Sign in / Sign up

Export Citation Format

Share Document