scholarly journals Stigmatic exudate in the Annonaceae: Pollinator reward, pollen germination medium or extragynoecial compitum?

2017 ◽  
Vol 59 (12) ◽  
pp. 881-894 ◽  
Author(s):  
Jenny Y. Y. Lau ◽  
Chun-Chiu Pang ◽  
Lawrence Ramsden ◽  
Richard M. K. Saunders
Keyword(s):  
Pollen Germination ◽  
Germination Medium ◽  
Stigmatic Exudate ◽  
Pollen Germination Medium

Development of pollen germination medium to test pollen viability of eggplant and its wild species

2018 ◽  
Vol 75 (2) ◽  
pp. 237
Author(s):  
P. Jayaprakash ◽  
D Sheeba ◽  
V.K. Vikas ◽  
M Sivasamy ◽  
T. Sabesan
Keyword(s):  
Wild Species ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Germination Medium ◽  
Pollen Germination Medium

scholarly journals Effects of various carbohydrates on the in vitro pollen germination of Vinca rosea and Cucumis melo var. utilissimus

2020 ◽  
Author(s):  
P. Manchanda ◽  
S. Sharma ◽  
A.D. Marni
Keyword(s):  
Cucumis Melo ◽  
Pollen Germination ◽  
Primary Source ◽  
Germination Percentage ◽  
Hanging Drop ◽  
Vinca Rosea ◽  
Germination Medium ◽  
Hanging Drop Method ◽  
Pollen Germination Medium

AbstractPollen germination is crucial for the process of plant development which strongly depends on the presence of carbohydrates as a primary source of energy. In this study, we analyzed the differential effects of four sugars with varying concentrations on the pollen germination of Vinca rosea Linn. and Cucumis melo var. utilissimus Duth. & Fuller. using Brewbaker and Kwack’s medium as germination medium and hanging drop method after an incubation period of 1h. Sucrose and glucose supported the pollen germination but galactose and fructose did not support and even considerably inhibited the pollen germination of Vinca rosea. Whereas in pollen germination of Cucumis melo var. utilissimus, all the four sugars supported the pollen germination. The study suggests that 15% sucrose, for Vinca rosea, and 12% galactose, for Cucumis melo var. utilissimus, supports in achieving the highest pollen germination percentage when added to the pollen germination medium.

scholarly journals Putrescine Increases Effective Pollination Period in Roses

1992 ◽  
Vol 2 (2) ◽  
pp. 211-213 ◽  
Author(s):  
Serge Gudin ◽  
Laurence Aréne
Keyword(s):  
Pollen Germination ◽  
Pollen Grains ◽  
Pollen Tubes ◽  
Rosa Hybrida ◽  
In Vitro Pollen Germination ◽  
Stigmatic Exudate ◽  
Germinated Pollen ◽  
Number Of Seeds

Flowers of two cultivars of Rosa hybrida were treated or not with putrescine before being pollinated from 2 to 8 days after anther emasculation. On both cultivars the 10-3 M putrescine treatment extended the effective pollination period, as shown by the best hip formation rates and mean number of seeds per hip. On one cultivar, the 10-5 M putrescine treatment increased fertilization efficiency (more hips obtained). The effect of putrescine was proportionally more important on the cultivar characterized by the highest stigmatic exudate pH. Putrescine also influenced in vitro pollen germination by increasing the length of emitted pollen tubes (10-3 and 10-5 M-putrescine) and the quantity of germinated pollen grains (10-5 M putrescine).

scholarly journals Pollen germination of the walnut cultivar ‛Geisenheim 286’ on different culture media

2014 ◽  
Vol 59 (1) ◽  
pp. 53-61
Author(s):  
Dragan Jankovic ◽  
Sladjana Jankovic ◽  
Svetlana Paunovic ◽  
Bratislav Cirkovic ◽  
Zoran Jovanovic
Keyword(s):  
Boric Acid ◽  
Calcium Chloride ◽  
Pollen Germination ◽  
Culture Media ◽  
Culture In Vitro ◽  
Agar Concentration ◽  
Germination Medium ◽  
Pollen Germinability

Pollen of the walnut cultivar ?Geisenheim 286(27.8%) when the germination medium contained 0.8% of agar, 15% of sucrose, 600 ppm of ? was cultured on germination media containing all possible combinations of sucrose (10, 15 and 20%); agar (0.6 and 0.8%); boric acid (0, 300 and 600 ppm) and calcium chloride (0, 50 and 100 ppm). A total of 54 different combinations of germination media were tested in an attempt to establish a suitable culture in vitro pollen germinability The interactions of the concentrations of agar and calcium chloride, boric acid and sucrose, calcium chloride and sucrose, as well as those of boric acid, calcium chloride and sucrose were significant. Pollen germination was maximized media for studying of the walnut. Significant differences in pollen germination were observed in response to changing concentrations of sucrose, boric acid and calcium chloride, but germination was not affected by changes in agar concentration. boric acid and 50 ppm of calcium chloride.

scholarly journals Effect of pH on in vitro Pollen Germination of Fourteen Cultivated and Wild Species of Cucurbit

1970 ◽  
Vol 17 ◽  
pp. 129-133 ◽  
Author(s):  
M Rashed Zaman
Keyword(s):  
Pollen Germination ◽  
Germination Rate ◽  
Pollen Grains ◽  
Culture Technique ◽  
In Vitro Pollen Germination ◽  
Effect Of Ph ◽  
Germination Medium ◽  
Ph Level ◽  
Wax Gourd

Context: The pH of the in vitro germination medium is the important factor controlling pollen germination and pollen tube development in different plant speciesObjectives: The aim of the present study was to assess the effect of pH levels of germination medium on the germinability 14 species of cucurbit.Materials and Methods: In vitro pollen germination of 14 cucurbit species was examined at five different levels of pH using Brewbaker and Kwack's medium. The pH of the medium was adjusted to 7.0, 7.5, 8.0, 8.5 and 9.0 by using a digital pH meter with the help of 0.1 N HCl and 0.1 N NaOH. In vitro pollen germination was tested using sitting drop culture technique. Five sitting drop cultures of pollen grains of five different pH for each species were raised on five different microslides. Then the slides were placed in a humidity chamber and incubated at 22 ± 2 °C for 1 to 2 hrs. All the cultures were fixed by adding a drop of a 1-2% acetocarmine before start scoring and the germinated and non-germinated pollen grains were scored using a microscope.Results: The pollen germination rate was higher in increased pH level in most of the cucurbit species studied and at 9.0 pH the maximum germination was observed in pumpkin (98%), snake gourd (98%), ridge gourd (96%), pointed gourd (89%), cucumber (98%), bitter gourd (96%), sweet gourd (98%) and sponge gourd (93%). The highest percentage of germination was observed in bottle gourd (98%) cucumber short (96%), watermelon (98%), muskmelon (97%) and ivy gourd (87%) at 8.5 pH level. The only cucurbit species wax gourd showed highest germination (97%) at the lower pH level of 7.5. Analysis of variance for the 14 cucurbits at five different pH levels showed that the differences between the germination rates of pollen grains of 14 cucurbit species at different pH level were highly significant (F=12.49, P<0.001, at 13/52 df). Conclusion: In most of the investigated cucurbit species pH level 8.5 to 9.0 was found optimum for the highest number of in vitro pollen germination, while wax gourd responded maximum pollen germination at pH 7.5.Key words: cucurbit; pollen; germination; pH.DOI: 10.3329/jbs.v17i0.7120J. bio-sci. 17: 129-133, 2009

Changes in polyamine synthesis during anther development and pollen germination in tobacco (Nicotiana tabacum)

1994 ◽  
Vol 92 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Fatiha Chibi ◽  
Angel Jesus Matilla ◽  
Trinidad Angosto ◽  
Dolores Garrido
Keyword(s):  
Nicotiana Tabacum ◽  
Pollen Germination ◽  
Anther Development ◽  
Polyamine Synthesis
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