Pollen-stigma interactions in Brassica oleracea; a new pollen germination medium and its use in elucidating the mechanism of self incompatibility

1983 ◽  
Vol 65 (3) ◽  
pp. 231-238 ◽  
Author(s):  
I. N. Roberts ◽  
T. C. Gaude ◽  
G. Harrod ◽  
H. G. Dickinson
Keyword(s):  
Brassica Oleracea ◽  
Pollen Germination ◽  
Self Incompatibility ◽  
Germination Medium ◽  
Pollen Germination Medium

Development of pollen germination medium to test pollen viability of eggplant and its wild species

2018 ◽  
Vol 75 (2) ◽  
pp. 237
Author(s):  
P. Jayaprakash ◽  
D Sheeba ◽  
V.K. Vikas ◽  
M Sivasamy ◽  
T. Sabesan
Keyword(s):  
Wild Species ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Germination Medium ◽  
Pollen Germination Medium

scholarly journals Effects of various carbohydrates on the in vitro pollen germination of Vinca rosea and Cucumis melo var. utilissimus

2020 ◽  
Author(s):  
P. Manchanda ◽  
S. Sharma ◽  
A.D. Marni
Keyword(s):  
Cucumis Melo ◽  
Pollen Germination ◽  
Primary Source ◽  
Germination Percentage ◽  
Hanging Drop ◽  
Vinca Rosea ◽  
Germination Medium ◽  
Hanging Drop Method ◽  
Pollen Germination Medium

AbstractPollen germination is crucial for the process of plant development which strongly depends on the presence of carbohydrates as a primary source of energy. In this study, we analyzed the differential effects of four sugars with varying concentrations on the pollen germination of Vinca rosea Linn. and Cucumis melo var. utilissimus Duth. & Fuller. using Brewbaker and Kwack’s medium as germination medium and hanging drop method after an incubation period of 1h. Sucrose and glucose supported the pollen germination but galactose and fructose did not support and even considerably inhibited the pollen germination of Vinca rosea. Whereas in pollen germination of Cucumis melo var. utilissimus, all the four sugars supported the pollen germination. The study suggests that 15% sucrose, for Vinca rosea, and 12% galactose, for Cucumis melo var. utilissimus, supports in achieving the highest pollen germination percentage when added to the pollen germination medium.

scholarly journals Stigmatic exudate in the Annonaceae: Pollinator reward, pollen germination medium or extragynoecial compitum?

2017 ◽  
Vol 59 (12) ◽  
pp. 881-894 ◽  
Author(s):  
Jenny Y. Y. Lau ◽  
Chun-Chiu Pang ◽  
Lawrence Ramsden ◽  
Richard M. K. Saunders
Keyword(s):  
Pollen Germination ◽  
Germination Medium ◽  
Stigmatic Exudate ◽  
Pollen Germination Medium

scholarly journals Coevolution of theS-Locus GenesSRK,SLGandSP11/SCRinBrassica oleraceaandB. rapa

Genetics ◽  
2002 ◽  
Vol 162 (2) ◽  
pp. 931-940 ◽  
Author(s):  
Keiichi Sato ◽  
Takeshi Nishio ◽  
Ryo Kimura ◽  
Makoto Kusaba ◽  
Tohru Suzuki ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Gene Conversion ◽  
Phylogenetic Trees ◽  
Hypervariable Region ◽  
Recognition System ◽  
Self Incompatibility ◽  
Male And Female ◽  
Diversifying Selection ◽  
Tight Linkage ◽  
Nonsynonymous Site

AbstractBrassica self-incompatibility (SI) is controlled by SLG and SRK expressed in the stigma and by SP11/SCR expressed in the anther. We determined the sequences of the S domains of 36 SRK alleles, 13 SLG alleles, and 14 SP11 alleles from Brassica oleracea and B. rapa. We found three S haplotypes lacking SLG genes in B. rapa, confirming that SLG is not essential for the SI recognition system. Together with reported sequences, the nucleotide diversities per synonymous and nonsynonymous site (πS and πN) at the SRK, SLG, and SP11 loci within B. oleracea were computed. The ratios of πN:πS for SP11 and the hypervariable region of SRK were significantly >1, suggesting operation of diversifying selection to maintain the diversity of these regions. In the phylogenetic trees of 12 SP11 sequences and their linked SRK alleles, the tree topology was not significantly different between SP11 and SRK, suggesting a tight linkage of male and female SI determinants during the evolutionary course of these haplotypes. Genetic exchanges between SLG and SRK seem to be frequent; three such recent exchanges were detected. The evolution of S haplotypes and the effect of gene conversion on self-incompatibility are discussed.

Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.

2019 ◽  
Vol 51 (7) ◽  
pp. 723-733 ◽  
Author(s):  
Songmei Shi ◽  
Qiguo Gao ◽  
Tonghong Zuo ◽  
Zhenze Lei ◽  
Quanming Pu ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Signaling Pathway ◽  
Chromosomal Region ◽  
The Self ◽  
Mature Stage ◽  
Self Incompatibility ◽  
Receptor Kinase ◽  
S Locus ◽  
Phylogenic Tree ◽  
Brassica Oleracea L

Abstract Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway.

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