scholarly journals A novel tomato SUMO E3 ligase, SlSIZ1, confers drought tolerance in transgenic tobacco

2017 ◽  
Vol 59 (2) ◽  
pp. 102-117 ◽  
Author(s):  
Song Zhang ◽  
Kunyang Zhuang ◽  
Shiju Wang ◽  
Jinlian Lv ◽  
Na'na Ma ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals SUMO E3 Ligase AtMMS21 Regulates Drought Tolerance in Arabidopsis thaliana F

2013 ◽  
Vol 55 (1) ◽  
pp. 83-95 ◽  
Author(s):  
Shengchun Zhang ◽  
Yanli Qi ◽  
Ming Liu ◽  
Chengwei Yang
Keyword(s):  
Arabidopsis Thaliana ◽  
Drought Tolerance ◽  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Baohua CHU ◽  
Jia SUN ◽  
Huan DANG ◽  
Ziqing MA ◽  
Shuang ZHAO ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals Overexpression of the pitaya phosphoethanolamine N-methyltransferase gene (HpPEAMT) enhanced simulated drought stress in tobacco

2021 ◽  
Author(s):  
Ai-Hua Wang ◽  
Lan Yang ◽  
Xin-Zhuan Yao ◽  
Xiao-Peng Wen
Keyword(s):  
Drought Stress ◽  
Stress Response ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Transgenic Tobacco ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Transgenic Tobacco Plants ◽  
Wild Type ◽  
Tobacco Plants

AbstractPhosphoethanolamine N-methyltransferase (PEAMTase) catalyzes the methylation of phosphoethanolamine to produce phosphocholine and plays an important role in the abiotic stress response. Although the PEAMT genes has been isolated from many species other than pitaya, its role in the drought stress response has not yet been fully elucidated. In the present study, we isolated a 1485 bp cDNA fragment of HpPEAMT from pitaya (Hylocereus polyrhizus). Phylogenetic analysis showed that, during its evolution, HpPEAMT has shown a high degree of amino acid sequence similarity with the orthologous genes in Chenopodiaceae species. To further investigate the function of HpPEAMT, we generated transgenic tobacco plants overexpressing HpPEAMT, and the transgenic plants accumulated significantly more glycine betaine (GB) than did the wild type (WT). Drought tolerance trials indicated that, compared with those of the wild-type (WT) plants, the roots of the transgenic plants showed higher drought tolerance ability and exhibited improved drought tolerance. Further analysis revealed that overexpression of HpPEAM in Nicotiana tabacum resulted in upregulation of transcript levels of GB biosynthesis-related genes (NiBADH, NiCMO and NiSDC) in the leaves. Furthermore, compared with the wild-type plants, the transgenic tobacco plants displayed a significantly lower malondialdehyde (MDA) accumulation and higher activities of the superoxide dismutase (SOD) and peroxidase (POD) antioxidant enzymes under drought stress. Taken together, our results suggested that HpPEAMT enhanced the drought tolerance of transgenic tobacco.

scholarly journals The RanBP2/RanGAP1∗SUMO1/Ubc9 Complex Is a Multisubunit SUMO E3 Ligase

2012 ◽  
Vol 46 (3) ◽  
pp. 287-298 ◽  
Author(s):  
Andreas Werner ◽  
Annette Flotho ◽  
Frauke Melchior
Keyword(s):  
E3 Ligase ◽  
Sumo E3 Ligase

scholarly journals Cbx4 Governs HIF-1α to Potentiate Angiogenesis of Hepatocellular Carcinoma by Its SUMO E3 Ligase Activity

Cancer Cell ◽  
2014 ◽  
Vol 25 (4) ◽  
pp. 547-548
Author(s):  
Jie Li ◽  
Ying Xu ◽  
Xi-Dai Long ◽  
Wei Wang ◽  
Hui-Ke Jiao ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
E3 Ligase ◽  
Sumo E3 Ligase

Zinc-finger protein MdBBX7/MdCOL9, a target of MdMIEL1 E3 ligase, confers drought tolerance in apple

2021 ◽  
Author(s):  
Pengxiang Chen ◽  
Fang Zhi ◽  
Xuewei Li ◽  
Wenyun Shen ◽  
Mingjia Yan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Negative Regulator ◽  
Binding Motif ◽  
Ethylene Response Factor ◽  
Finger Protein

Abstract Water deficit is one of the main challenges for apple (Malus × domestica) growth and productivity. Breeding drought-tolerant cultivars depends on a thorough understanding of the drought responses of apple trees. Here, we identified the zinc-finger protein B-BOX 7/CONSTANS-LIKE 9 (MdBBX7/MdCOL9), which plays a positive role in apple drought tolerance. The overexpression of MdBBX7 enhanced drought tolerance, whereas knocking down MdBBX7 expression reduced it. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified one cis-element of MdBBX7, CCTTG, as well as its known binding motif, the T/G box. ChIP-seq and RNA-seq identified 1,197 direct targets of MdBBX7, including ETHYLENE RESPONSE FACTOR (ERF1), EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), and GOLDEN2-LIKE 1 (GLK1) and these were further verified by ChIP-qPCR and electronic mobility shift assays. Yeast two-hybrid screen identified an interacting protein of MdBBX7, RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1). Further examination revealed that MdMIEL1 could mediate the ubiquitination and degradation of MdBBX7 by the 26S proteasome pathway. Genetic interaction analysis suggested that MdMIEL1 acts as an upstream factor of MdBBX7. In addition, MdMIEL1 was a negative regulator of the apple drought stress response. Taken together, our results illustrate the molecular mechanisms by which the MdMIEL1–MdBBX7 module influences the response of apple to drought stress.

scholarly journals The SUMO E3 Ligase MdSIZ1 Targets MdbHLH104 to Regulate Plasma Membrane H+-ATPase Activity and Iron Homeostasis

2018 ◽  
Vol 179 (1) ◽  
pp. 88-106 ◽  
Author(s):  
Li-Jie Zhou ◽  
Chun-Ling Zhang ◽  
Rui-Fen Zhang ◽  
Gui-Luan Wang ◽  
Yuan-Yuan Li ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Atpase Activity ◽  
Iron Homeostasis ◽  
E3 Ligase ◽  
Sumo E3 Ligase

The RanBP2 SUMO E3 ligase is neither HECT- nor RING-type

2004 ◽  
Vol 11 (10) ◽  
pp. 984-991 ◽  
Author(s):  
Andrea Pichler ◽  
Puck Knipscheer ◽  
Hisato Saitoh ◽  
Titia K Sixma ◽  
Frauke Melchior
Keyword(s):  
E3 Ligase ◽  
Ring Type ◽  
Sumo E3 Ligase
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