H2 O2 -induced Leaf Cell Death and the Crosstalk of Reactive Nitric/Oxygen SpeciesF

2013 ◽  
Vol 55 (3) ◽  
pp. 202-208 ◽  
Author(s):  
Yiqin Wang ◽  
Aihong Lin ◽  
Gary J. Loake ◽  
Chengcai Chu
Keyword(s):  
1997 ◽  
Vol 9 (8) ◽  
pp. 1397 ◽  
Author(s):  
Christoph Peterhansel ◽  
Andreas Freialdenhoven ◽  
Joachim Kurth ◽  
Regina Kolsch ◽  
Paul Schulze-Lefert
Keyword(s):  

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Misako Himeno ◽  
Yugo Kitazawa ◽  
Tetsuya Yoshida ◽  
Kensaku Maejima ◽  
Yasuyuki Yamaji ◽  
...  
Keyword(s):  

1997 ◽  
pp. 1397-1409 ◽  
Author(s):  
C. Peterhansel ◽  
A. Freialdenhoven ◽  
J. Kurth ◽  
R. Kolsch ◽  
P. Schulze-Lefert
Keyword(s):  

2011 ◽  
Vol 158 (1) ◽  
pp. 451-464 ◽  
Author(s):  
Aihong Lin ◽  
Yiqin Wang ◽  
Jiuyou Tang ◽  
Peng Xue ◽  
Chunlai Li ◽  
...  

Author(s):  
Anne F. Bushnell ◽  
Sarah Webster ◽  
Lynn S. Perlmutter

Apoptosis, or programmed cell death, is an important mechanism in development and in diverse disease states. The morphological characteristics of apoptosis were first identified using the electron microscope. Since then, DNA laddering on agarose gels was found to correlate well with apoptotic cell death in cultured cells of dissimilar origins. Recently numerous DNA nick end labeling methods have been developed in an attempt to visualize, at the light microscopic level, the apoptotic cells responsible for DNA laddering.The present studies were designed to compare various tissue processing techniques and staining methods to assess the occurrence of apoptosis in post mortem tissue from Alzheimer's diseased (AD) and control human brains by DNA nick end labeling methods. Three tissue preparation methods and two commercial DNA nick end labeling kits were evaluated: the Apoptag kit from Oncor and the Biotin-21 dUTP 3' end labeling kit from Clontech. The detection methods of the two kits differed in that the Oncor kit used digoxigenin dUTP and anti-digoxigenin-peroxidase and the Clontech used biotinylated dUTP and avidinperoxidase. Both used 3-3' diaminobenzidine (DAB) for final color development.


Author(s):  
S. Trachtenberg ◽  
P.M. Steinert ◽  
B.L. Trus ◽  
A.C. Steven

During terminal differentiation of vertebrate epidermis, certain specific keratin intermediate filament (KIF) proteins are produced. Keratinization of the epidermis involves cell death and disruption of the cytoplasm, leaving a network of KIF embedded in an amorphous matrix which forms the outer horny layer known as the stratum corneum. Eventually these cells are shed (desquamation). Normally, the processes of differentiation, keratinization, and desquamation are regulated in an orderly manner. In psoriasis, a chronic skin disease, a hyperkeratotic stratum corneum is produced, resulting in abnormal desquamation of unusually large scales. In this disease, the normal KIF proteins are diminished in amount or absent, and other proteins more typical of proliferative epidermal cells are present. There is also evidence of proteolytic degradation of the KIF.


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