Promotion of wound healing by acetate in murine colonic epithelial cell via c‐Jun N‐terminal kinase activation

2020 ◽  
Vol 35 (7) ◽  
pp. 1171-1179 ◽  
Author(s):  
Takahiro Nakano ◽  
Kazuhiko Uchiyama ◽  
Chihiro Ushiroda ◽  
Saori Kashiwagi ◽  
Yuki Toyokawa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Colonic Epithelial Cell ◽  
Kinase Activation

Partially hydrolyzed guar gum enhances colonic epithelial wound healing via activation of RhoA and ERK1/2

2016 ◽  
Vol 7 (7) ◽  
pp. 3176-3183 ◽  
Author(s):  
Yusuke Horii ◽  
Kazuhiko Uchiyama ◽  
Yuki Toyokawa ◽  
Yuma Hotta ◽  
Makoto Tanaka ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Guar Gum ◽  
Colonic Epithelial Cell ◽  
Epithelial Wound Healing ◽  
Partially Hydrolyzed Guar Gum

We provide evidence that partially hydrolyzed guar gum (PHGG) enhances colonic epithelial cell wound healing.

Tu1296 BILE ACIDS DELAY THE WOUND HEALING OF COLONIC EPITHELIAL CELL THROUGH TRANSMEMBRANE G-PROTEIN-COUPLED RECEPTOR 5

2020 ◽  
Vol 158 (6) ◽  
pp. S-1047-S-1048
Author(s):  
Yuka Azuma ◽  
Kazuhiko Uchiyama ◽  
Mariko Kajiwara ◽  
Satoshi Sugino ◽  
Hiroaki Kitae ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Bile Acids ◽  
G Protein ◽  
Colonic Epithelial Cell ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled

scholarly journals A Novel Pharmacological Approach to Enhance the Integrity and Accelerate Restitution of the Intestinal Epithelial Barrier

2020 ◽  
Vol 26 (9) ◽  
pp. 1340-1352
Author(s):  
Xuelei Cao ◽  
Lei Sun ◽  
Susana Lechuga ◽  
Nayden G Naydenov ◽  
Alex Feygin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Cell Monolayers ◽  
Barrier Disruption ◽  
Epithelial Cell Monolayers ◽  
Immunofluorescence Labeling ◽  
E Cadherin

Abstract Background Disruption of the gut barrier is an essential mechanism of inflammatory bowel diseases (IBDs) contributing to the development of mucosal inflammation. A hallmark of barrier disruption is the disassembly of epithelial adherens junctions (AJs) driven by decreased expression of a major AJ protein, E-cadherin. A group of isoxazole compounds, such as E-cadherin-upregulator (ECU) and ML327, were previously shown to stimulate E-cadherin expression in poorly differentiated human cancer cells. This study was designed to examine whether these isoxazole compounds can enhance and protect model intestinal epithelial barriers in vitro. Methods The study was conducted using T84, SK-CO15, and HT-29 human colonic epithelial cell monolayers. Disruption of the epithelial barrier was induced by pro-inflammatory cytokines, tumor necrosis factor-α, and interferon-γ. Barrier integrity and epithelial junction assembly was examined using different permeability assays, immunofluorescence labeling, and confocal microscopy. Epithelial restitution was analyzed using a scratch wound healing assay. Results E-cadherin-upregulator and ML327 treatment of intestinal epithelial cell monolayers resulted in several barrier-protective effects, including reduced steady-state epithelial permeability, inhibition of cytokine-induced barrier disruption and junction disassembly, and acceleration of epithelial wound healing. Surprisingly, these effects were not due to upregulation of E-cadherin expression but were mediated by multiple mechanisms including inhibition of junction protein endocytosis, attenuation of cytokine-induced apoptosis, and activation of promigratory Src and AKT signaling. Conclusions Our data highlight ECU and ML327 as promising compounds for developing new therapeutic strategies to protect the integrity and accelerate the restitution of the intestinal epithelial barrier in IBD and other inflammatory disorders.

Cytokine changes in gastric and colonic epithelial cell in response to Planta ovata extract

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Javed Yakoob ◽  
Wasim Jafri ◽  
Malik Hassan Mehmood ◽  
Zaigham Abbas ◽  
Kanwal Tariq
Keyword(s):  
Epithelial Cell ◽  
Dna Fragmentation ◽  
Cell Lines ◽  
Inflammatory Reaction ◽  
Sw480 Cell ◽  
Colonic Epithelial Cell ◽  
Ags Cells ◽  
Sw480 Cells

AbstractBackgroundPsyllium (MethodsThe AGS cells and SW480 cells were pretreated with Ispaghul extract in concentrations, i. e. 3.5 and 7 μg/mL prior to infection withResultsDNA fragmentation in AGS and SW480 cells treated with Ispaghul extract was not significant (2.3±0.8 %) compared with untreated cells (2.2±0.6 %). Ispaghul extract decreased theConclusionsIspaghul extract had an effect on stimulated secretion of IL-1β by the AGS and SW480 cell. It decreased pro-inflammatory reaction from both cell lines stimulated by bacteria.

scholarly journals Oestrogen and colonic epithelial cell growth.

Gut ◽  
1995 ◽  
Vol 37 (6) ◽  
pp. 737-739 ◽  
Author(s):  
S Singh ◽  
M J Langman
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Colonic Epithelial Cell
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