Prevalence and population analysis of Vibrio parahaemolyticus in retail aquatic products from the southern Fujian coast, China

2020 ◽  
Vol 40 (5) ◽  
Author(s):  
Yuanqing Hu ◽  
Fengxia Li ◽  
Ying Zheng ◽  
Zhichao Zeng ◽  
Guoguang Zhang ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Population Analysis ◽  
Aquatic Products ◽  
Fujian Coast

scholarly journals Prevalence, characterization, and antibiotic susceptibility of Vibrio parahaemolyticus isolated from retail aquatic products in North China

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiaoke Xu ◽  
Jianheng Cheng ◽  
Qingping Wu ◽  
Jumei Zhang ◽  
Tengfei Xie
Keyword(s):  
Vibrio Parahaemolyticus ◽  
North China ◽  
Antimicrobial Agents ◽  
Foodborne Pathogen ◽  
Eastern Coast ◽  
Most Probable Number ◽  
Antibiotic Resistant ◽  
Probable Number ◽  
Aquatic Products ◽  
Resistance Patterns

Abstract Background Vibrio parahaemolyticus is a major foodborne pathogen, particularly in Asian countries. Increased occurrence of outbreaks of V. parahaemolyticus gastroenteritis in China indicates the need to evaluation of the prevalence of this pathogenic species. V. parahaemolyticus distribution in shellfish from the eastern coast of China has been reported previously. However, to date, the prevalence of V. parahaemolyticus in retail aquatic products in North China has not been determined. To investigate the prevalence of V. parahaemolyticus in aquatic products in North China, 260 aquatic product samples were obtained from retail markets in 6 provinces of North China from November to December in 2012 and July to August in 2013. Results V. parahaemolyticus was detected in 94 (36.2 %) of the samples by the most probable number method. The density of V. parahaemolyticus ranged from 1.50 to 1100 MPN/g. V. parahaemolyticus was detected at a rate of 50.0 % and 22.7 % in summer and in winter, respectively. The density of V. parahaemolyticus was significantly higher in summer than in winter, with mean levels of 16.5 MPN/g and 5.0 MPN/g, respectively. Among 145 V. parahaemolyticus isolates examined, none of the isolates possessed tdh and trh. In multiplex PCR-based O-antigen serotyping of these 145 isolates, all serotypes, other than O6, O7, and O9, were detected, and serotype O2 was found to be the most prevalent (detected in 54 isolates). The 145 isolates were grouped into 7 clusters by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) at a similarity coefficient of 0.66. The antimicrobial resistance patterns of these 145 isolates to 12 antimicrobial agents revealed that most of the isolates were resistant to streptomycin (86.2 %), while fewer were resistant to ampicillin (49.6 %), cefazolin (43.5 %), cephalothin (35.9 %), and kanamycin (22.1 %). All of the examined isolates were susceptible to azithromycin and chloramphenicol. Conclusions The findings of this study will help in defining appropriate monitoring programs, understanding of the dissemination of antibiotic resistant strains, and providing information for the assessment of exposure to this microorganism at the consumption level.

Comparison on the growth heterogeneity of Vibrio parahaemolyticus coupled with strain sources and genotypes analyses in different oligotrophic conditions

2021 ◽  
Author(s):  
Jun Shi ◽  
Wei Zhao ◽  
Jing Xie ◽  
Yongheng Zhu ◽  
Yingjie Pan ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Specific Growth ◽  
Growth Curves ◽  
Gompertz Model ◽  
Growth Variability ◽  
Aquatic Products ◽  
Theoretical Support ◽  
Food Borne ◽  
Modified Gompertz Model

Vibrio parahaemolyticus is an important food-borne pathogen in aquatic products, which can survive long-term in an oligotrophic environment and maintain pathogenicity. In this study, the growth curves of 38 strains of V.parahaemolyticus (pathogenic and environmental strains) under different oligotrophic conditions (tryptone soy broth (TSB), TSB diluted 2, 4, and 6 times medium) were simulated and their growth heterogeneity was compared. The growth kinetic parameters (maximum specific growth rate ( µ max ) and lag time (LT)) were calculated by the modified Gompertz model. The results showed that oligotrophic conditions affected the growth variability of strains, and the coefficient of variation (CV) of all strains reached the maximum in the 4-fold dilution of TSB. Under different oligotrophic conditions, the LT of the pathogenic strains was shorter than that of the environmental strains, while the µ max of the environmental strains was greater. This indicated that pathogenic strains were more adaptable to the nutrient-deficient environment. The analysis of different genotypes revealed that the strains with genotype tlh + /tdh + /trh − showed a greater growth variability in oligotrophic environments. These results provided theoretical support for the accuracy of the risk assessment of aquatic products.

Prevalence and Population Analysis of Vibrio parahaemolyticus Isolated from Freshwater Fish in Zhejiang Province, China

2021 ◽  
Vol 18 (2) ◽  
pp. 139-146
Author(s):  
Honghu Chen ◽  
Shilei Dong ◽  
Yu Yan ◽  
Li Zhan ◽  
Junyan Zhang ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
Vibrio Parahaemolyticus ◽  
Population Analysis ◽  
Zhejiang Province

scholarly journals Prevalence and population analysis ofVibrio parahaemolyticusin aquatic products from South China markets

2015 ◽  
Vol 362 (22) ◽  
pp. fnv178 ◽  
Author(s):  
Tengfei Xie ◽  
Qingping Wu ◽  
Xiaoke Xu ◽  
Jumei Zhang ◽  
Weipeng Guo
Keyword(s):  
South China ◽  
Population Analysis ◽  
Aquatic Products

scholarly journals Population analysis of Vibrio parahaemolyticus originating from different geographical regions demonstrates a high genetic diversity

2014 ◽  
Vol 14 (1) ◽  
pp. 59 ◽  
Author(s):  
Sara Urmersbach ◽  
Thomas Alter ◽  
Madura Sanjeevani Koralage ◽  
Lisa Sperling ◽  
Gunnar Gerdts ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Vibrio Parahaemolyticus ◽  
Population Analysis ◽  
High Genetic Diversity ◽  
Geographical Regions
Sign in / Sign up

Export Citation Format

Share Document