scholarly journals Comparative Proteomics and Secretomics Revealed Virulence and Antibiotic Resistance-Associated Factors in Vibrio parahaemolyticus Recovered From Commonly Consumed Aquatic Products

2020 ◽  
Vol 11 ◽  
Author(s):  
Zhuoying Zhu ◽  
Lianzhi Yang ◽  
Pan Yu ◽  
Yongjie Wang ◽  
Xu Peng ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Parahaemolyticus ◽  
Associated Factors ◽  
Comparative Proteomics ◽  
Aquatic Products

scholarly journals Draft Genome Sequence of the Shrimp Pathogen Vibrio parahaemolyticus ST17.P5-S1, Isolated in Peninsular Malaysia

2018 ◽  
Vol 7 (11) ◽  
Author(s):  
Sridevi Devadas ◽  
Subha Bhassu ◽  
Tze Chiew Christie Soo ◽  
Fatimah M. Yusoff ◽  
Mohamed Shariff
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Vibrio Parahaemolyticus ◽  
East Coast ◽  
Draft Genome ◽  
Antibiotic Resistance Genes ◽  
Peninsular Malaysia ◽  
Penaeus Vannamei ◽  
Content Type ◽  
Acute Hepatopancreatic Necrosis Disease

We sequenced the genome of Vibrio parahaemolyticus strain ST17.P5-S1, isolated from Penaeus vannamei cultured in the east coast of Peninsular Malaysia. The strain contains several antibiotic resistance genes and a plasmid encoding the Photorhabdus insect-related (Pir) toxin-like genes, pirAvp and pirBvp, associated with acute hepatopancreatic necrosis disease (AHPND).

scholarly journals Genomic basis of antibiotic resistance in Vibrio parahaemolyticus strain JPA1

2019 ◽  
Vol 114 ◽  
Author(s):  
Felipe Hernandes Coutinho ◽  
Diogo Antonio Tschoeke ◽  
Maysa Mandetta Clementino ◽  
Cristiane Carneiro Thompson ◽  
Fabiano Lopes Thompson
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Parahaemolyticus ◽  
Parahaemolyticus Strain ◽  
Genomic Basis

Antibiotic resistance associated with aquaculture in Vietnam

2016 ◽  
Vol 37 (3) ◽  
pp. 108 ◽  
Author(s):  
Hoang Nam Kha Nguyen ◽  
Thi Thu Hao Van ◽  
Peter J Coloe
Keyword(s):  
Sustainable Development ◽  
Antibiotic Resistance ◽  
Food Supply ◽  
Health Management ◽  
Therapeutic Use ◽  
Human Medicine ◽  
The Third ◽  
Aquatic Products ◽  
Food Fish ◽  
Food Animals

The fishery sector is an important source of income, employment and food supply in Vietnam. In 2014, Vietnam was ranked the world's fourth largest exporter and the third largest producer of farmed food fish. Vietnam seafood export has attained the value of over US$6.0 billion since 2011 and reached a peak of US$7.9 billion in 2014. However, many problems and diseases confront sustainable development of the fishery sector and overuse of antibiotics is considered a major challenge. Antibiotics are used in aquaculture for both therapeutic and prophylactic reasons. Various antimicrobials used in human medicine are also used for food animals even for non-therapeutic use. The use of antibiotics in health management of aquaculture farming is of great concern due to possible residues in aquatic products and in the development of antibiotic resistance. In 2005 the Vietnamese government first promulgated a list of medicines, chemicals and antibiotics that are banned or limited for use in aquaculture and this is regularly updated and amended to tackle the growing problem of antibiotic resistance.

scholarly journals Occurrence and Antibiotic Resistance of Vibrio parahaemolyticus from Shellfish in Selangor, Malaysia

2015 ◽  
Vol 6 ◽  
Author(s):  
Vengadesh Letchumanan ◽  
Priyia Pusparajah ◽  
Loh Teng-Hern Tan ◽  
Wai-Fong Yin ◽  
Learn-Han Lee ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Parahaemolyticus

scholarly journals Genomic and evolutionary features of two AHPND positive Vibrio parahaemolyticus strains isolated from shrimp (Penaeus monodon) of south-west Bangladesh

2019 ◽  
Author(s):  
Shawon Ahmmed ◽  
Md. Abdullah-Al-Kamran Khan ◽  
Md. Mostavi Enan Eshik ◽  
Nusrat Jahan Punom ◽  
Abul B.M.M.K. Islam ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Vibrio Parahaemolyticus ◽  
Early Stage ◽  
Antibiotic Resistance Genes ◽  
Secretion System ◽  
Economic Losses ◽  
Emergence Pattern ◽  
Toxin Genes ◽  
South West ◽  
Evolutionary Features

Abstract Background Due to its rapid lethal effect in the early stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) causing great economic losses, since it first outbreak in southeast China in 2009. Vibrio parahaemolyticus , carrying the pir A and pir B toxin genes is known to cause AHPND in shrimp. The overall objective of this study was to sequence whole genome of AHPND positive V. parahaemolyticus strains isolated from shrimp ( Peneaus monodon ) of south-west region of Bangladesh in 2016 and 2017 and characterize the genomic features and emergence pattern of this marine pathogen. Results Two targeted AHPND positive V. parahaemolyticus strains were confirmed using PCR with 16S rRNA, ldh , AP3 and AP4 primers. The assembled genomes of strain MSR16 and MSR17 were comprised of total 5,393,740 bp and 5,241,592 bp, respectively. From annotation, several virulence genes involved in chemotaxis and motility, EPS type II secretion system, Type three secretion system-1 (TTSS-1) and its secreted effectors, thermolabile hemolysin were found in both strains. Importantly, the ~69 kb plasmid was identified in both MSR16 and MSR17 strains containing the two toxin genes pir A and pir B. Antibiotic resistance genes were predicted against β-lactam, fluoroquinolone, tetracycline, macrolide and cephalosporin groups in both MSR16 and MSR17 strains. Conclusions The findings of this research may facilitate the tracking of pathogenic and/or antibiotic resistance V. parahaemolyticus isolates between production sites, and the identification of candidate strains for production of vaccines as an aid to control of this devastating disease. Also, the emergence pattern of this pathogen can be highlighted to determine the characteristic differences of other strains found all over the world.

scholarly journals Prevalence, characterization, and antibiotic susceptibility of Vibrio parahaemolyticus isolated from retail aquatic products in North China

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiaoke Xu ◽  
Jianheng Cheng ◽  
Qingping Wu ◽  
Jumei Zhang ◽  
Tengfei Xie
Keyword(s):  
Vibrio Parahaemolyticus ◽  
North China ◽  
Antimicrobial Agents ◽  
Foodborne Pathogen ◽  
Eastern Coast ◽  
Most Probable Number ◽  
Antibiotic Resistant ◽  
Probable Number ◽  
Aquatic Products ◽  
Resistance Patterns

Abstract Background Vibrio parahaemolyticus is a major foodborne pathogen, particularly in Asian countries. Increased occurrence of outbreaks of V. parahaemolyticus gastroenteritis in China indicates the need to evaluation of the prevalence of this pathogenic species. V. parahaemolyticus distribution in shellfish from the eastern coast of China has been reported previously. However, to date, the prevalence of V. parahaemolyticus in retail aquatic products in North China has not been determined. To investigate the prevalence of V. parahaemolyticus in aquatic products in North China, 260 aquatic product samples were obtained from retail markets in 6 provinces of North China from November to December in 2012 and July to August in 2013. Results V. parahaemolyticus was detected in 94 (36.2 %) of the samples by the most probable number method. The density of V. parahaemolyticus ranged from 1.50 to 1100 MPN/g. V. parahaemolyticus was detected at a rate of 50.0 % and 22.7 % in summer and in winter, respectively. The density of V. parahaemolyticus was significantly higher in summer than in winter, with mean levels of 16.5 MPN/g and 5.0 MPN/g, respectively. Among 145 V. parahaemolyticus isolates examined, none of the isolates possessed tdh and trh. In multiplex PCR-based O-antigen serotyping of these 145 isolates, all serotypes, other than O6, O7, and O9, were detected, and serotype O2 was found to be the most prevalent (detected in 54 isolates). The 145 isolates were grouped into 7 clusters by enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) at a similarity coefficient of 0.66. The antimicrobial resistance patterns of these 145 isolates to 12 antimicrobial agents revealed that most of the isolates were resistant to streptomycin (86.2 %), while fewer were resistant to ampicillin (49.6 %), cefazolin (43.5 %), cephalothin (35.9 %), and kanamycin (22.1 %). All of the examined isolates were susceptible to azithromycin and chloramphenicol. Conclusions The findings of this study will help in defining appropriate monitoring programs, understanding of the dissemination of antibiotic resistant strains, and providing information for the assessment of exposure to this microorganism at the consumption level.

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