A Survey on Biofilm Formation of Lactobacillus rhamnosus (PTCC 1637) and Lactobacillus plantarum (PTCC 1745) as a Survival Strategy of Probiotics Against Antibiotic in Vitro and Yogurt

2021 ◽  
Author(s):  
Zeinab Rezaei ◽  
Saeid Khanzadi ◽  
Amir Salari
Keyword(s):  
Lactobacillus Plantarum ◽  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
Survival Strategy

scholarly journals Impact of Environmental and Genetic Factors on Biofilm Formation by the Probiotic Strain Lactobacillus rhamnosus GG

2007 ◽  
Vol 73 (21) ◽  
pp. 6768-6775 ◽  
Author(s):  
Sarah Lebeer ◽  
Tine L. A. Verhoeven ◽  
M�nica Perea V�lez ◽  
Jos Vanderleyden ◽  
Sigrid C. J. De Keersmaecker
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Lipoteichoic Acid ◽  
Microtiter Plate ◽  
Phenotypic Analysis ◽  
Lactobacillus Rhamnosus Gg ◽  
Abiotic Surfaces

ABSTRACTLactobacillus rhamnosusGG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover,L. rhamnosusGG displays very good in vitro adherence to epithelial cells and mucus. Here, we report thatL. rhamnosusGG is able to form biofilms on abiotic surfaces, in contrast to other strains of theLactobacillus caseigroup tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation byL. rhamnosusGG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (wzb), lipoteichoic acid (dltD), and central metabolism (luxS) showed their relative importance in biofilm formation byL. rhamnosusGG.

scholarly journals Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 797 ◽  
Author(s):  
Gianfranco Pannella ◽  
Silvia Jane Lombardi ◽  
Francesca Coppola ◽  
Franca Vergalito ◽  
Massimo Iorizzo ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Malic Acid ◽  
Life Style ◽  
Biofilm Formation ◽  
Stress Conditions ◽  
Producer Strain ◽  
Planktonic Form ◽  
Strain Dependent ◽  
Producer Strains

Biofilm life-style of Lactobacillus plantarum (L. plantarum) strains was evaluated in vitro as a new and suitable biotechnological strategy to assure L-malic acid conversion in wine stress conditions. Sixty-eight L. plantarum strains isolated from diverse sources were assessed for their ability to form biofilm in acid (pH 3.5 or 3.2) or in ethanol (12% or 14%) stress conditions. The effect of incubation times (24 and 72 h) on the biofilm formation was evaluated. The study highlighted that, regardless of isolation source and stress conditions, the ability to form biofilm was strain-dependent. Specifically, two clusters, formed by high and low biofilm producer strains, were identified. Among high producer strains, L. plantarum Lpls22 was chosen as the highest producer strain and cultivated in planktonic form or in biofilm using oak supports. Model wines at 12% of ethanol and pH 3.5 or 3.2 were used to assess planktonic and biofilm cells survival and to evaluate the effect of biofilm on L-malic acid conversion. For cells in planktonic form, a strong survival decay was detected. In contrast, cells in biofilm life-style showed high resistance, assuring a prompt and complete L-malic acid conversion.

scholarly journals Propriedades probióticas in vitro de Lactobacillus spp. isolados de queijos minas artesanais da Serra da Canastra - MG

2014 ◽  
Vol 66 (5) ◽  
pp. 1592-1600 ◽  
Author(s):  
C.R.G. Andrade ◽  
M.R. Souza ◽  
C.F.A.M. Penna ◽  
L.B. Acurcio ◽  
F.M. Sant'Anna ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Lactobacillus Plantarum ◽  
Enterococcus Faecalis ◽  
Salmonella Enterica ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Spp

O objetivo deste estudo foi determinar o potencial probiótico in vitro de Lactobacillus spp. isolados de queijos minas artesanais da Serra da Canastra, considerando-se o antagonismo entre amostras isoladas frente a microrganismos indicadores, a susceptibilidade a antimicrobianos, a sensibilidade ao ácido gástrico e a sensibilidade a sais biliares. Todas as bactérias ácido-lácticas testadas apresentaram resistência ao ácido gástrico (pH 2,0) e aos sais biliares (0,3%), bem como atividade antagonista contra Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, Salmonella enterica var. Typhimurium, Enterococcus faecalis e bactérias ácido-lácticas isoladas dos próprios queijos - Lactobacillus plantarum (D27) e Lactobacillus rhamnosus (B25). Todas as amostras foram sensíveis à eritromicina e tetraciclina e resistentes à ciprofloxacina, gentamicina, oxacilina, estreptomicina e vancomicina. L. plantarum(B17) apresentou melhor potencial probiótico, pois obteve resultados satisfatórios em todas as propriedades avaliadas. Mais estudos são necessários para verificar a presença e a capacidade de transmissão de genes de resistência antimicrobiana a outros microrganismos e para avaliar o potencial dos microrganismos in vivo. As bactérias selecionadas poderão ser utilizadas na elaboração de queijos em que sejam mantidos o sabor e a tradição do queijo minas artesanal do estado de Minas Gerais.

scholarly journals Lactobacillus rhamnosus Interferes with Candida albicans Adherence and Biofilm Formation: A Potential Alternative Treatment of Candidiasis

2021 ◽  
Vol 9 (2) ◽  
Author(s):  
Ribeiro FC ◽  
◽  
Barros PP ◽  
Jorge AOC ◽  
Iglesias MC ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Alternative Treatment ◽  
Biofilm Formation ◽  
Therapeutic Agent ◽  
Lactobacillus Rhamnosus ◽  
Living Cells ◽  
Probiotic Bacteria ◽  
Potential Alternative ◽  
Potential Use

The objective of the present study was to evaluate the ability of Lactobacillus rhamnosus, on different preparations (living lactobacilli, dead by heat lactobacilli and supernatant of lactobacilli suspension), to interfere with Candida albicans adherence to ephitelial cells and biofilm formation. The results showed a reduction of 66.2% in the number of Candida cells adhered to epithelial cells, when the suspension of living L. rhamnosus was used. On the same way, this suspension reduced the in vitro biofim formation by C. albicans. In conclusion, the suspension with living cells of L. rhamnosus was able to reduce the ability of C. albicans to adhere on ephitelial cells and to form biofilm, suggesting a potential use of this probiotic bacteria as a therapeutic agent in candidiasis.

scholarly journals A Survival Strategy for Pseudomonas aeruginosa That Uses Exopolysaccharides To Sequester and Store Iron To Stimulate Psl-Dependent Biofilm Formation

2016 ◽  
Vol 82 (21) ◽  
pp. 6403-6413 ◽  
Author(s):  
Shan Yu ◽  
Qing Wei ◽  
Tianhu Zhao ◽  
Yuan Guo ◽  
Luyan Z. Ma
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Iron Concentration ◽  
Survival Strategy ◽  
Iron Storage ◽  
Content Type ◽  
High Level ◽  
Biofilm Development ◽  
Biofilm Matrix

ABSTRACTExopolysaccharide Psl is a critical biofilm matrix component inPseudomonas aeruginosa, which forms a fiber-like matrix to enmesh bacterial communities. Iron is important forP. aeruginosabiofilm development, yet it is not clearly understood how iron contributes to biofilm development. Here, we showed that iron promoted biofilm formation via elevating Psl production inP. aeruginosa. The high level of iron stimulated the synthesis of Psl by reducing rhamnolipid biosynthesis and inhibiting the expression of AmrZ, a repressor ofpslgenes. Iron-stimulated Psl biosynthesis and biofilm formation held true in mucoidP. aeruginosastrains. Subsequent experiments indicated that iron bound with Pslin vitroand in biofilms, which suggested that Psl fibers functioned as an iron storage channel inP. aeruginosabiofilms. Moreover, among three matrix exopolysaccharides ofP. aeruginosa, Psl is the only exopolysaccharide that can bind with both ferrous and ferric ion, yet with higher affinity for ferrous iron. Our data suggest a survival strategy ofP. aeruginosathat uses exopolysaccharide to sequester and store iron to stimulate Psl-dependent biofilm formation.IMPORTANCEPseudomonas aeruginosais an environmental microorganism which is also an opportunistic pathogen that can cause severe infections in immunocompromised individuals. It is the predominant airway pathogen causing morbidity and mortality in individuals affected by the genetic disease cystic fibrosis (CF). Increased airway iron and biofilm formation have been proposed to be the potential factors involved in the persistence ofP. aeruginosain CF patients. Here, we showed that a high level of iron enhanced the production of the key biofilm matrix exopolysaccharide Psl to stimulate Psl-dependent biofilm formation. Our results not only make the link between biofilm formation and iron concentration in CF, but also could guide the administration or use of iron chelators to interfere with biofilm formation inP. aeruginosain CF patients. Furthermore, our data also imply a survival strategy ofP. aeruginosaunder high-iron environmental conditions.

Inulin Induced Co-Aggregation of Saccharomyces boulardii with Potential Pathogenic Bacteria

2019 ◽  
Vol 14 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Ganguly Reya ◽  
Chakraborty Ritun ◽  
Sarkar Kasturi
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Saccharomyces Boulardii ◽  
Aggregate Formation ◽  
E Coli ◽  
Aggregation Property ◽  
Number Of Microorganisms ◽  
Subsequent Elimination

The synbiotic potential of Saccharomyces boulardii and inulin was evaluated on mice gut microflora. The number of microorganisms in the feces of mice treated with the synbiotic combination or Saccharomyces boulardii/inulin alone was found to be less compared to the normal control. S. boulardii grown in presence of inulin was found to interact and form aggregation with Escherichia coli and Enterococcus faecalis in vitro though the same interaction pattern was not seen with Staphylococcus aureus, Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus plantarum. Aggregate was not found between E. coli/E. faecalis and S. boularii when the later was grown in prebiotics like maltodextrin and fructooligosaccharide. All the microorganisms were found to be alive in the aggregate and heat treatment of S. boulardii completely abolished the aggregation property. There was no biofilm formation in the aggregate and inhibition of protein synthesis resulted in no aggregation with E. coli/E. faecalis. Removal of the effect of cycloheximide from S. boulardii restored back the aggregation in presence of inulin. Thus, the decreased number of microorganisms in feces might be a result of aggregate formation between S. boulardii and E. coli/E. faecalis in presence of inulin and subsequent elimination from gut through mucus.

In vitro resistance of Lactobacillus plantarum LP299v or Lactobacillus rhamnosus GG carried by vegetable appetizer

LWT ◽  
2019 ◽  
Vol 116 ◽  
pp. 108512 ◽  
Author(s):  
Priscilla Araújo Campos ◽  
Eliane Maurício Furtado Martins ◽  
Maurílio Lopes Martins ◽  
Aurélia Dornelas de Oliveira Martins ◽  
Bruno Ricardo de Castro Leite Júnior ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Lactobacillus Rhamnosus ◽  
Lactobacillus Rhamnosus Gg

scholarly journals KAJIAN ISOLAT BAKTERI ASAM LAKTAT DALAM MENURUNKAN KOLESTEROL SECARA IN VITRO DENGAN KEBERADAAN OLIGOSAKARIDA (A Study In Vitro of Lactic Acid Bacteria (LAB) Isolates on Cholesterol Lowering Ability in The Presence of Oligosaccharides)

2016 ◽  
Vol 36 (02) ◽  
pp. 196 ◽  
Author(s):  
Yati Maryati ◽  
Lilis Nuraida ◽  
Ratih Dewanti Hariyadi
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Carbon Source ◽  
Cell Surface ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Rhamnosus ◽  
Cholesterol Reduction ◽  
Cholesterol Binding

This work evaluated the abilities of five isolates of lactic acid bacteria (LAB) from different sources, i.e Lactobacillus fermentum S21209 and Lactobacillus plantarum 1-S27202 from tempe, Lactobacillus rhamnosus R23 and Pediococcus pentosaceus 1-A38 from human breast milk and a commercially available human isolates Lactobacillus acidophilus FNCC0051 in lowering cholesterol by in vitro and metabolizing the prebiotic oligosaccharide compounds. The effects of oligosaccharide compounds on the performance of the LAB isolates in lowering cholesterol were also evaluated. The tests were done in MRS based medium in vitro with or without oligosaccharides i.e. galactooligosacharrides (GOS), fructooligosaccharides (FOS), inulin, hydrolyzed inulin or combination of oligosaccharides as prebiotics. The results revealed that all isolates were able to reduce cholesterol in the medium, and the highest cholesterol reduction was observed for L. acidophilus FNCC0051 and L. rhamnosus R23. There are two different mechanism in the loweringof cholesterol; cholesterol assimilation and cholesterol binding on the cell surface. For the case of P. pentosaceus 1-A38, it involves the assimilation, while the other four isolates may involve cholesterol binding on the cell surface. In addition, the tested LAB’s has different ability to use prebiotics, as shown by the reduction of total sugar in the medium. Oligosaccharides metabolism by L. acidophilus FNCC0051 and L. rhamnosus R23 resulted in several organic acid and SCFA with lactic acid produced as the largest proportion followed by acetic acid. Furthermore, the proportion of propionic and butyric acids were influenced by the type of isolates and carbon source. L. acidophilus FNCC 0051 was able to reduce cholesterol in the MRS based medium with oligosaccharides and their combination as carbon source and cholesterol reducing ability seems to involve both assimilation and cholesterol binding on the cell surface.Keywords: Lactic acid bacteria (LAB), oligosaccharides, synbiotic, cholesterol reduction, prebioticsABSTRAKPenelitian ini mengevaluasi lima isolat bakteri asam laktat (BAL) dari sumber yang berbeda, yaitu Lactobacillus fermentum S21209 dan Lactobacillus plantarum 1-S27202 dari tempe, Lactobacillus rhamnosus R23 dan Pediococcus pentosaceus 1-A38 dari ASI dan isolat komersial Lactobacillus acidophilus FNCC 0051 dari percernaan manusia dalam kemampuannya menurunkan kolesterol secara in vitro dan kemampuannya memetabolisme senyawa oligosakarida prebiotik. Pengaruh senyawa oligosakarida terhadap kemampuan isolat BAL terpilih untuk menurunkan kolesterol juga dievaluasi. Pengujian dilakukan pada media berbasis MRS dengan atau tanpa oligosakarida terdiri dari galaktooligosakarida (GOS), fruktooligosakarida (FOS), inulin, hidrolisat inulin atau kombinasi oligosakarida sebagai prebiotik. Hasil penelitian menunjukkan bahwa semua isolat mampu menurunkan kolesterol, dan penurunan kolesterol tertinggi ditunjukkan oleh isolat L. acidophilus FNCC0051 dan L. rhamnosus R23. Penurunan kolesterol diduga terjadi melalui dua cara yang berbeda. Mekanisme penurunan kolesterol oleh isolat P. pentosaceus 1-A38 melibatkan asimilasi kolesterol, sedangkan pada keempat isolat lainnya kemungkinan melibatkan pengikatan kolesterol pada permukaan sel. Selain itu, isolat BAL juga memiliki kemampuan yang berbeda dalam memanfaatkan oligosakarida prebiotik, terlihat pada penurunan total gula dalam medium. Metabolisme senyawa oligosakarida oleh L. acidophilus FNCC0051 dan L. rhamnosus R23 menghasilkan beberapa asam organik termasuk SCFA dengan proporsi terbesar asam laktat diikuti oleh asam asetat. Selain itu, proporsi asam propionat dan butirat dipengaruhi oleh jenis isolat dan sumber karbon. L. acidophilus FNCC 0051 mampu menurunkan kolesterol dalam media berbasis MRS dengan keberadaan oligosakarida baik tunggal maupun kombinasi sebagai sumber karbon dan melibatkan mekanisme baik asimilasi dan pengikatan kolesterol pada permukaan sel.Kata kunci: Bakteri asam laktat (BAL), oligosakarida, sinbiotik, penurunan kolesterol, prebiotik

scholarly journals Combined effect of arginine and fluoride on the growth of Lactobacillus rhamnosus GG

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mohammed Nadeem Bijle ◽  
Manikandan Ekambaram ◽  
Edward C. M. Lo ◽  
Cynthia Kar Yung Yiu
Keyword(s):  
Biofilm Formation ◽  
Lactobacillus Rhamnosus ◽  
In Vitro Study ◽  
Lactobacillus Rhamnosus Gg ◽  
Biofilm Growth ◽  
Biofilm Thickness ◽  
Colony Forming Units ◽  
Monosodium Salt ◽  
Relative Gene

AbstractThe objectives of the in vitro study were: (1) to investigate the effect of combining L-arginine (Arg) and NaF on the growth of Lactobacillus rhamnosus GG (LRG); and (2) to identify an optimum synergistic concentration for the synbiotic (Arg + LRG)-fluoride (SF) therapy. 1% Arg + 2000-ppm NaF (A-SF) and 2% Arg + 2000-ppm NaF (B-SF) demonstrated antagonism against LRG (FIC > 4.0). Both XTT (2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) and WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) assays showed that A-SF and B-SF enhanced the growth of LRG when compared to 2000-ppm NaF and LRG control. Colony forming units, bacterial weight, and biofilm thickness of A-SF and B-SF were significantly higher than 2000-ppm NaF and LRG control. Biofilm imaging depicted that 2000-ppm NaF inhibited biofilm formation; while 1%/2% Arg, A-SF, and B-SF increased biofilm growth of LRG. Lactic acid formation was the lowest for 2000-ppm NaF, followed by A-SF and then B-SF. The SF buffer potential after 24 h was the highest for B-SF, and then A-SF. Biofilm pH for B-SF was closest to neutral. Fluoride, Arg and LRG bioavailability remained unaffected in B-SF. The relative gene expression for arcA, argG, and argH was significantly higher for B-SF than the respective controls. In conclusion, combining 2% Arg, 2000-ppm NaF, and LRG provides an optimum synbiotic-fluoride synergism.

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