Effect of Drought Stress Induced by PEG 6000 on Ocimum basilicum L. Aroma Profile

2021 ◽  
Author(s):  
Başar Sevindik ◽  
Onur Sevindik ◽  
Serkan Selli
Keyword(s):  
Drought Stress ◽  
Ocimum Basilicum ◽  
Aroma Profile ◽  
Peg 6000

Drought stress and osmo-priming effects on physiological and biochemical characteristics of Ocimum basilicum

2016 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
Bahram Majd Nassiry ◽  
Neda Mohammadi
Keyword(s):  
Drought Stress ◽  
Total Protein ◽  
Catalase Activity ◽  
Seedling Emergence ◽  
Seed Priming ◽  
Ocimum Basilicum ◽  
Field Development ◽  
Physiological And Biochemical Characteristics ◽  
Germination Characteristics ◽  
And Control

    One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objectives of this research were to evaluate the effects of osmo-priming on germination characteristics and changes of proline, protein and catalase activity of Ocimum basilicum seeds. Results showed that drought stress reduced the germination characteristics and drought stress in -8 bar was the critical stress.  Priming treatments were include KNO3, PEG and NaCl by 0, -4 and -8 bar concentrations. The seeds were primed with those materials for 8 and 16 hours. The highest germination characteristics were obtained from nitrate potassium in -8 bar for 16 hours priming. Therefore the best seed treatment under drought stress during germination was obtained from the osmo-primed with -8 bar nitrate potassium for 16 hours. The drought stress increased proline and catalase activity but reduced total protein. Priming treatment increases proline, total protein and catalase activity under drought and control conditions. It is concluded that priming results in improvement in germination components of Ocimum basilicum in drought stress conditions and increases the resistance to drought stress with improvement of proline, protein and catalase activity in germination phase.

scholarly journals Application of Phenotyping Methods in Detection of Drought and Salinity Stress in Basil (Ocimum basilicum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Boris Lazarević ◽  
Zlatko Šatović ◽  
Ana Nimac ◽  
Monika Vidak ◽  
Jerko Gunjača ◽  
...  
Keyword(s):  
Drought Stress ◽  
Salinity Stress ◽  
Vegetation Index ◽  
Normalized Difference Vegetation Index ◽  
Multispectral Imaging ◽  
Ocimum Basilicum ◽  
Phenotypic Traits ◽  
Severe Drought ◽  
Moderate Salinity ◽  
Automated Phenotyping

Basil is one of the most widespread aromatic and medicinal plants, which is often grown in drought- and salinity-prone regions. Often co-occurrence of drought and salinity stresses in agroecosystems and similarities of symptoms which they cause on plants complicates the differentiation among them. Development of automated phenotyping techniques with integrative and simultaneous quantification of multiple morphological and physiological traits enables early detection and quantification of different stresses on a whole plant basis. In this study, we have used different phenotyping techniques including chlorophyll fluorescence imaging, multispectral imaging, and 3D multispectral scanning, aiming to quantify changes in basil phenotypic traits under early and prolonged drought and salinity stress and to determine traits which could differentiate among drought and salinity stressed basil plants. Ocimum basilicum “Genovese” was grown in a growth chamber under well-watered control [45–50% volumetric water content (VWC)], moderate salinity stress (100 mM NaCl), severe salinity stress (200 mM NaCl), moderate drought stress (25–30% VWC), and severe drought stress (15–20% VWC). Phenotypic traits were measured for 3 weeks in 7-day intervals. Automated phenotyping techniques were able to detect basil responses to early and prolonged salinity and drought stress. In addition, several phenotypic traits were able to differentiate among salinity and drought. At early stages, low anthocyanin index (ARI), chlorophyll index (CHI), and hue (HUE2D), and higher reflectance in red (RRed), reflectance in green (RGreen), and leaf inclination (LINC) indicated drought stress. At later stress stages, maximum fluorescence (Fm), HUE2D, normalized difference vegetation index (NDVI), and LINC contribute the most to the differentiation among drought and non-stressed as well as among drought and salinity stressed plants. ARI and electron transport rate (ETR) were best for differentiation of salinity stressed plants from non-stressed plants both at early and prolonged stress.

scholarly journals PEG 6000-Stimulated Drought Stress Improves the Attributes of In Vitro Growth, Steviol Glycosides Production, and Antioxidant Activities in Stevia rebaudiana Bertoni

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1552
Author(s):  
Muhammad Arslan Ahmad ◽  
Rabia Javed ◽  
Muhammad Adeel ◽  
Muhammad Rizwan ◽  
Yuesuo Yang
Keyword(s):  
Drought Stress ◽  
Antioxidant Activities ◽  
Growth Parameters ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Stevia Rebaudiana ◽  
Total Phenolic ◽  
Steviol Glycosides ◽  
Peg 6000

This study addresses the favourable effects of drought stress imposed by polyethylene glycol (PEG) 6000 on the micropropagated shoots of Stevia rebaudiana. Various concentrations, i.e., 0, 0.5, 1, 2, and 4% of PEG 6000 were applied to the nodal shoot explants for four weeks, and the influence produced on shoots growth parameters, bioactive steviol glycosides (rebaudioside A and stevioside), and nonenzymatic antioxidant activities (total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC), total reducing power (TRP) and 1,1-diphenyl-2-picrylhydrazyl(DPPH)-free radical scavenging activity (FRSA)) was elucidated. The significantly highest yield (92.4% direct shoot organogenesis) and secondary metabolites (2.94% Reb A, 2.52% ST, 95.3% DPPH-FRSA, 15.0% TPC, 13.0 µg/mg TFC, 22.3 µg/mg TAC, and 19.8 µg/mg TRP) production in response to abiotic stress elicitors was obtained in Murashige and Skoog (MS) medium treatment provided with 4% of PEG 6000. The overall trend was significant enhancement of growth dynamics and pharmaceutical compounds from control to 4% of PEG 6000 concentration as a defensive response against reactive oxygen species (ROS) produced in excess by water deficit. This is a very promising study to be employed in bioreactors to get markedly enhanced content of compounds of medicinal importance in the pharmaceutical market.

scholarly journals Stomata Index of Cattleya sp. Lindl., Planlet in Drought-Stress Conditions

2019 ◽  
Vol 6 (1) ◽  
pp. 15-19
Author(s):  
Tara Sesafia Paletri ◽  
Endang Nurcahyani ◽  
Yulianty Yulianty ◽  
Rochmah Agustrina
Keyword(s):  
Drought Stress ◽  
Ethylene Glycol ◽  
Water Availability ◽  
Stress Conditions ◽  
Poly Ethylene Glycol ◽  
Factor B ◽  
Peg 6000 ◽  
Poly Ethylene ◽  
Concentration Levels

Cattleya was one of the most popular orchids by Indonesian. Constraints in cultivating Cattleya included the lack of water availability in an area, causing drought stress. Stress in drought in vitro can be simulated by adding Poly Ethylene Glycol 6000 and growth regulators in atonic form. The purpose was to determine the effect of atonic solution and PEG 6000 on the stomata index toward in vitro drought stress conditions. This study was carried out in a 3 x 3 factorial experiment. Factor A was an atonic solution with 3 concentration levels: 0 mL/L, 2 mL/L, and 3 mL/L. Factor B is PEG 6000 with 3 concentration levels: 0% b/v, 20% b/v, 25% b/v. The results showed that the higher the concentration of PEG 6000 and atonic concentrations caused a decrease in the stomata index of Cattleya orchid plantlets under drought stress conditions in vitro.

scholarly journals The Study of Early Selection Method through Vigor and Viability Parameter in Citrus amblycarpa (Hassk) under Salinity and Drought Stress Condition

2018 ◽  
Vol 1 (1) ◽  
pp. 1
Author(s):  
Anggi Nindita ◽  
Choirul Umam ◽  
Siti Fatimah ◽  
Faiza Suwarno
Keyword(s):  
Drought Stress ◽  
Stress Condition ◽  
Selection Method ◽  
Early Selection ◽  
Sand Soil ◽  
Seed Moisture Content ◽  
Talcum Powder ◽  
Peg 6000 ◽  
The Third ◽  
Drought Stress Condition

The study was aimed to obtain information regarding the early selection method of Limau lime under saline and drought condition including the proper method for seed extraction, observation period in germination testing, and type of germination media. The experiment was conducted from March 2015 to January 2016 at the Laboratory of Seed Science and Technology, Department of Agronomy and Horticulture, Faculty of Agriculture, Bogor Agricultural University. It consisted of four main experiments. The first experiment was the extraction method on Limaulime seed using distilled water, ash from the husk, talcum powder, and HCl 1%. The second experiment was germination testing of lime seed using three media i.e. sand, the combination of sand: soil (1:1) (v:v), and towel tissue. The third experiment has tested the vigor of Limau lime seeds in different saline conditions by using NaCl, i.e. 1500 ppm, 3000 ppm, and 4500 ppm. The fourth experiment, tested the vigor of Limau lime seeds in drought stress condition using PEG 6000, i.e. 0, - 0.75 bar, -1.5 bar, and -2.25 bar. The result of the first experiment showed that the treatments had no significantly different effect on all variables observed except for the seed moisture content. The second experiment revealed that the best germination media was elucidated from sand treatment and sand: soil (1:1) with the first and the second count at 20th and 29th day respectively. The result of the third and four experiment elucidated that the higher concentration of NaCl (Salinity stress condition) or PEG 6000 (drought stress condition), the smaller the germination percentage of Limau Lime’s seeds.

scholarly journals Purple basil (Ocimum basilicum var. purple) oil under drought stress effects on Aspergillus fumigates fungus in the food industry

2021 ◽  
Author(s):  
Arghavan Haji Mohammad ◽  
Hesam Sharifi Nejad
Keyword(s):  
Drought Stress ◽  
Essential Oil ◽  
Essential Oils ◽  
Food Industry ◽  
Field Capacity ◽  
Ocimum Basilicum ◽  
Research Station ◽  
Minimum Concentration ◽  
Aspergillus Fumigates ◽  
Basil Oil

Abstract To investigate the effect of drought stress Purple Basil (Ocimum basilicum var.purple), on Aspergillus fumigates in food industry, an experiment was performed in the Alborz Karaj research station, and extracted essential oils of purple basil in Agricultural Education Center of Imam Khomeini in 2011, wasperformed. Purple basil essential oil obtained from the stress levels of 30%, 60% and 90% field capacity (FC) is. Using brows microdilution, the average minimum inhibitory concentration and the mean minimum concentration of essential oils produced in concentrations of 0/25, 0/5, 1, 1/5, 2 and 2/5 mg/ml against fungi Aspergillus fumigates was determined in three replicates. The results showed that under conditions of purple basil oil 30% field capacity had the strongest activity against the fungi studied (0/37 mg/ml = 90MIC).Antibacterial activity of essential oil and the lowest was seen in 90% of field capacity (0/97mg/ml = 90MIC). Purple basil oil under the terms of the MFC as the lowest 30% of field capacity (0/56 mg/ml) and maximum (0/99 mg/ml) was seen in 90% of the crop. According to survey results in the absence of purple basil oil 30% field capacity (severe water stress) was a good performance against fungi Aspergillus fumigates antifungal and take advantage of it to use its influence in the food industry, pharmaceutical and health is .

scholarly journals Physiological responses and P5CS gene expression of transgenic oil palm plantlet induced by drought stress

2020 ◽  
Vol 88 (2) ◽  
Author(s):  
. TURHADI ◽  
Hayati MINARSIH ◽  
Imron RIYADI ◽  
. PRIYONO ◽  
Asmini BUDIANI
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Oil Palm ◽  
Elaeis Guineensis ◽  
Proline Content ◽  
Limiting Factors ◽  
Total Chlorophyll ◽  
Peg 6000 ◽  
P5cs Gene ◽  
Transgenic Plantlets

Drought is one of the limiting factors in crop cultivation, such as in oil palm (Elaeis guineensis Jacq.). The transgenic approaches are expected to increase plant tolerance to drought stress and minimize low productivity when drought occurs. Proline is an osmoprotectant compound in plants which its biosynthesis involved the P5CS gene. The objective of this study was to evaluate the tolerance level of P5CS-transgenic oil palm to drought stress induced by polyethylene glycol 6000 (PEG-6000). In this present study, the transgenic and non-transgenic oil palms were treated by  0, 2, and 4% PEG-6000 under in vitro conditions. The experiment was arranged as a factorial completely randomized design with three replications. The drought level score, total chlorophyll content, carotenoids, and proline content, as well as P5CS gene expression in leaf tissues were observed at 7 and 14 days after stress treatments. The result showed that transgenic plantlets had a lower drought level score than those of non-transgenic lines. A concentration of 4% PEG-6000 treatment reduced the total chlorophyll and carotenoids contents than that of 2% concentration in non-transgenic plantlets at 7 and 14 day after treatments (DAT). In addition, proline content and P5CS gene expression level in transgenic had been significantly increased during stress treatment. Based on these results, it can be concluded that the P5CS transgene increased the drought stress tolerance of oil palm.

Influence of Peg-induced Drought Stress on Antioxidant Components of Callus Tissue of Sainfoin (Onobrychis viciifolia Scop.) Ecotypes

2020 ◽  
Author(s):  
Ramazan Beyaz ◽  
Mustafa Yıldız
Keyword(s):  
Drought Stress ◽  
Callus Tissue ◽  
Stress Factors ◽  
Antioxidant Enzyme Activities ◽  
Ms Medium ◽  
Peg 6000 ◽  
Onobrychis Viciifolia ◽  
Stress Effects ◽  
Abiotic Stress Factors ◽  
Effects Of Drought

Background: Drought is one of the important abiotic stress factors that restrict plant development. Sainfoin is known to be relatively tolerant of drought. However, there are limited reports on the effects of drought stress on antioxidant components of Onobrychis species and/or ecotypes. Methods: To determine drought stress effects on antioxidant components of 4 sainfoin ecotypes (“Koçaþ”, “Malya”, “Altýnova” and “Ulaþ”), callus tissue was grown on MS medium enriched with 200 g l-1 PEG-6000. Result: Callus of the sainfoin ecotypes, cultured on a medium having 200 g l-1 PEG-6000, showed significant increases in antioxidant enzyme activities (SOD, CAT, GR (except in “Ulaþ” ecotype) and APX). However, the PEG induced increase in the accumulation of MDA and proline in callus tissue of all sainfoin ecotypes. The findings of the present study show that in terms of the increasing rate of antioxidant components under drought stress, the “Koçaþ” ecotype seemed to be the best.

Sign in / Sign up

Export Citation Format

Share Document