Glucose oxidase (GOX) release of stressed chia mucilage‐sodium alginate capsules prepared by Electrospraying

2021 ◽  
Author(s):  
Minerva Rentería‐Ortega ◽  
Ma. Paz Salgado‐Cruz ◽  
Eduardo Morales‐Sánchez ◽  
Liliana Alamilla‐Beltrán ◽  
Mariana Valdespino‐León ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Sodium Alginate ◽  
Alginate Capsules ◽  
Chia Mucilage

scholarly journals The Effect of Extrusion Voltage and Flowrate to the Viability and Survivability of Probiotic L. casei Encapsulated in Alginate-Chitosan

2021 ◽  
Vol 9 (3) ◽  
Author(s):  
Djaenudin ◽  
Endang Saepudin ◽  
Muhamad Nasir
Keyword(s):  
Sodium Alginate ◽  
Flow Rate ◽  
Liquid Flow Rate ◽  
Chitosan Solution ◽  
Gastric Fluid ◽  
Simulated Gastric Fluid ◽  
Chitosan Beads ◽  
Alginate Capsules ◽  
Encapsulation Process ◽  
Extrusion Method

 Chitosan-coated L. casei containing alginate capsules (shortened as L. casei capsules) were prepared by extruding L. casei containing alginate solution at different extrusion voltage and and flow rate followed by coating the wet capsules in chitosan solution. This study aimed to determine the effect of extrusion voltage and sodium alginate liquid flow rate on the viability of L. casei bacteria in the encapsulation process. The encapsulation process in this study was carried out by the extrusion method using sodium alginate of 1% (w/v) and chitosan of 0.2% (w/v). The resulted beads were immersed in a simulated gastric fluid (SGF) (NaCl 0.2%; HCl 0.5 M with a pH of 1.5) for 1, 60, and 120 min at 37 °C. The number of L. casei cells before encapsulation was 12.3 log CFU. After encapsulation, the maximum viability of L. Casei obtained by voltage variations of 0 kV and flow rate 5 mL/min were 12.26 log CFU.  After testing the beads in SGF for 1 min, the results obtained indicate that viability of L.casei in the sodium alginate - chitosan beads with an extrusion voltage of 0 kV and 5 mL/min was 11.8 log CFU/g. The result indicated that encapsulated L. casei in the sodium alginate - chitosan beads with a voltage of 0 kV and 5 mL/min was the highest survivability level of 97.38 %. The conclusions of the study were The higher extrusion voltage can kill more L. casei while the higher extrusion flow rate can protect more L. casei.

Glucose oxidase-mediated sodium alginate gelation: Equipment-Free detection of glucose in fruit samples

2021 ◽  
Vol 148 ◽  
pp. 109805
Author(s):  
Hao Zhang ◽  
Xiuzhu Li ◽  
Zheng-Ming Qian ◽  
Shengpeng Wang ◽  
Feng-Qing Yang
Keyword(s):  
Glucose Oxidase ◽  
Sodium Alginate ◽  
Fruit Samples

Effects of Process Variables on Size of Chitosan-Alginate Capsules through Extrusion-Dripping Method

2014 ◽  
Vol 925 ◽  
pp. 8-12 ◽  
Author(s):  
Gim Pao Lim ◽  
Hui Yen Ong ◽  
Boon Beng Lee ◽  
Muhammad Syarhabil Ahmad ◽  
Harbant Singh ◽  
...  
Keyword(s):  
Sodium Alginate ◽  
Calcium Chloride ◽  
Concentration Ratio ◽  
Inner Core ◽  
Gelation Time ◽  
Chloride Concentration ◽  
Membrane Thickness ◽  
Process Variables ◽  
Alginate Capsules ◽  
Alginate Concentration

The objective of this study was to investigate the effects of process variables on size of chitosan-alginate capsules produced through extrusion-dripping method. Chitosan-calcium chloride solution was extruded into sodium alginate to form chitosan-alginate capsules which have a defined inner core and membrane. The results showed that sodium alginate concentration, chitosan concentration, chitosan to calcium chloride concentration ratio, needle diameter, gelation time have significant effect on the diameter and membrane thickness of chitosan-alginate capsules. Under the tested condition, chitosan-alginate capsules with diameter in a range of 3.4 mm to 4.5 mm were produced. The membrane thickness of the capsules increased when the curing time increased from 5 min to 60 min. As the concentration of sodium alginate (0.3-0.9 %w/v) and chitosan (0.4-1.0 %w/v) increased, the diameter of the chitosan-alginate capsules increased. The diameter of chitosan-alginate capsules increased with decreasing the chitosan to calcium chloride concentration ratio (1:0.5-1:1.5).

scholarly journals Technological Features of Production Calcium-Alginate Microcapsules for Self-Healing Asphalt

2018 ◽  
Vol 251 ◽  
pp. 01008 ◽  
Author(s):  
Sergey Inozemtcev ◽  
Evgeniy Korolev
Keyword(s):  
Sodium Alginate ◽  
Calcium Alginate ◽  
Reducing Agent ◽  
Self Healing ◽  
Single Drop ◽  
Alginate Capsules ◽  
Formulation Factors ◽  
Suspension Preparation

The effect of the formulation factors of calcium-alginate suspension preparation on the properties of calcium-alginate microcapsules was studied. The maximum useful volume of the reducing agent is set it was established, up to 93.8% of the total volume, in microcapsules, from the beating, to retain 2.31% sodium alginate and 10.5% reducing agent. The proposed mechanism for the formation of calcium-alginate capsules, which occurs due to shrinkage during the drying of a single drop of suspension, breaking the emulsion and forming an integral shell with a nucleus from the reducing agent.

scholarly journals Effects of sodium alginate capsules as 3D scaffolds on hormones and genes expression in preantral follicles of mice compared to 2D medium: An experimental study

2020 ◽  
Author(s):  
Cyrus Jalili ◽  
Fuzieh Khani Hemmatabadi ◽  
Kamran Mansouri ◽  
Mehrdad Bakhtiyari
Keyword(s):  
Sodium Alginate ◽  
In Vitro Maturation ◽  
Fetal Bovine Serum ◽  
Minimal Essential Medium ◽  
3D Scaffolds ◽  
Preantral Follicles ◽  
Alginate Capsules ◽  
Genes Expression ◽  
Fetal Bovine

Background: The improvement of in vitro maturation methods, which can activate the preantral follicle growth, plays a crucial role in the production of mature oocytes in reproductive technology. Objective: To evaluate the different concentrations of 3D scaffolds of sodium alginate on hormones and gene expression in mice preantral follicles. Materials and Methods: Immature female BALB/c mice (12-14 days) were sacrificed. The follicles were removed mechanically and transferred into α minimal essential medium with 5% fetal bovine serum. The preantral follicles were incubated with different concentrations of sodium alginate (0.25%, 0.5%, and 1%) and 2D medium for 12 days. The follicles were examined for antral formation following the 10th day and the diameter on days 6th and 12th. The levels of hormones (AMH, androstenedione, 17

scholarly journals FLOATING GASTRORETENTIVE OF AMOXICILLIN USING HARD ALGINATE CAPSULES AND ITS ANTIBACTERIAL ACTIVITIES

2017 ◽  
Vol 10 (5) ◽  
pp. 413
Author(s):  
Hakim Bangun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Sustained Release ◽  
Sodium Alginate ◽  
Solid Dispersion ◽  
Antibacterial Activities ◽  
X Ray Diffraction ◽  
X Ray ◽  
Alginate Capsules ◽  
Pvp K30

Objective: The aim of this study was to formulate floating gastroretentive of amoxicillin  using hard alginate capsules shell and to evaluate antibacterial activities of floating gastroretentive of amoxicillin.Methods: Alginate capsules shell were made by using sodium alginate 80-120 cP. Amoxicillin was prepared in solid dispersion to obtain sustained release requirement for 12 hours. Solid dispersion was prepared by solvent method using polyvinylpyrrolidone (PVP) K30. The solid dispersion was characterized by  X-ray diffraction and FTIR analysis.  The drug release test was carried out by using USP paddle method in simulated gastric fluid. Concentrations of amoxicillin were determined by using spectrophotometer UV at 272 nm. The antibacterial activity of  aliquots  dissolution were assessed by using agar plate diffusion method against Staphylococcus aureus and Escherichia coli as bacterial model.Results: The alginate capsules shell were made from sodium alginate 80-120 cP with size 0.  The dissolution test results showed that amoxicillin in the form of solid dispersions with weight  ratio amoxicillin with PVP K30 was 1:1 provided sustained release of amoxicillin during 12 hours, while the release of amoxicillin without solid dispersion was too slow.   The floating lag time was 0 minute and floating time was more than 12 hours. The X-ray diffraction pattern of amoxicillin solid dispersion had amorphous shape. Antibacterial activity test showed that the dissolution aliquots of amoxicillin solid dispersion were effective against  Staphylococcus aureus and Escherichia coli.Conclusions: Based on the results of this study, it is concluded that the alginate capsules shell can be used for preparation of floating gastroretentive of amoxicillin using amoxicillin solid dispersion and the dissolution aliqouts give antibacterial effect.Keywords: Floating; gastroretentive; alginate capsule; amoxicillin; solid dispersion; release; antibacterial  

scholarly journals Shape Tuning and Size Prediction of Millimeter-Scale Calcium-Alginate Capsules with Aqueous Core

Polymers ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 688
Author(s):  
Jinchao Zhao ◽  
Qing Guo ◽  
Wei Huang ◽  
Teng Zhang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Surface Tension ◽  
Sodium Alginate ◽  
Mechanical Stability ◽  
Methyl Cellulose ◽  
Quantitative Relationship ◽  
Average Diameter ◽  
Poloxamer 407 ◽  
Alginate Capsules ◽  
Spherical Caps ◽  
Aqueous Core

Controllable feature and size, good mechanical stability and intelligent release behavior is the capsule products relentless pursuit of the goal. In addition, to illustrate the quantitative relationship of structure and performance is also important for encapsulation technology development. In this study, the sphericity and size of millimeter-scale calcium sodium alginate capsules (mm-CaSA-Caps) with aqueous core were well tuned by manipulating the viscosity, surface tension, and density of CaCl2/carboxyl methyl cellulose (CMC) drops and sodium alginate (SA) solution. The well-tuned mm-CaSA-Caps showed significant mechanical and control-releasing property effects. The results showed that the prepared mm-CaSA-Caps were highly monodispersed with average diameter from 3.8 to 4.8 mm. The viscosity of the SA solution and the viscosity and surface tension of the CaCl2/CMC solution had significant effects on the mm-CaSA-Caps sphericity. Uniform and spherical mm-CaSA-Caps could be formed with high viscosity CaCl2/CMC solution (between 168.5 and 917.5 mPa·s), low viscosity SA solution (between 16.2 and 72.0 mPa·s) and decreased surface tension SA solution (by adding 0.01 wt.% poloxamer 407). The diameter of the mm-CaSA-Caps could be predicted by a modified Tate’s law, which correlated well with the experimental data. The Caps with sphericity factor (SF) < 0.07 had better mechanical stability, with the crushing force 2.91–15.5 times and the surface Young’s modulus 2.1–3.99 times higher than those of the non-spherical Caps (SF > 0.07). Meanwhile, the spherical Caps had a more even permeation rate, which was helpful in producing uniform and sustained releasing applications in foodstuff, medicine, agriculture and chemical industry.

Improvement of the stability of glucose oxidase via encapsulation in sodium alginate–cellulose sulfate–poly(methylene-co-guanidine) capsules

2007 ◽  
Vol 41 (6-7) ◽  
pp. 748-755 ◽  
Author(s):  
Alica Vikartovská ◽  
Marek Bučko ◽  
Danica Mislovičová ◽  
Vladimír Pätoprstý ◽  
Igor Lacík ◽  
...  
Keyword(s):  
Glucose Oxidase ◽  
Sodium Alginate ◽  
Cellulose Sulfate ◽  
The Stability

scholarly journals Behavior of lateral buds of Hancornia speciosa after cryopreservation by encapsulation-vitrification

2017 ◽  
Vol 39 (1) ◽  
pp. 87
Author(s):  
Débora De Oliveira Prudente ◽  
Renato Paiva ◽  
Fernanda Carlota Nery ◽  
Wesley Pires Flausino Máximo ◽  
Luciano Coutinho Silva
Keyword(s):  
Sodium Alginate ◽  
Plant Material ◽  
Endemic Area ◽  
Survival Rates ◽  
Economic Potential ◽  
Lateral Buds ◽  
Alginate Capsules ◽  
High Regeneration ◽  
Hancornia Speciosa

 Hancornia speciosa is a fruitful species from Cerrado biome with high economic potential. However, the intense and disordered extractivism have caused a reduction of its population in its endemic area. In addition, seed recalcitrance negatively affects the conventional conservation of the species. Aiming to find alternatives that enable the long-term conservation of this species, the study’s objective was to assess the behavior of lateral bud’s regrowth after cryopreservation procedures by encapsulation-vitrification technique. Sodium alginate capsules containing lateral buds were pre-cultured in liquid WPM supplemented with 1.0 M glycerol, and subsequently exposed to different concentrations of sucrose (0.3; 0.75 and 1.0 M) for 24 or 48 hours. The capsules were subjected to dehydration in silica gel or airflow hood for 0, 1, 2 and 3 hours before different incubation times in PVS2 (0, 15, 30, 60 and 120 minutes) at 0°C. A high regeneration percentage of lateral buds was observed after cryopreservation of capsules treated with 0.75 M sucrose plus 1.0 M glycerol (24 hours), associated with dehydration in an airflow hood (1 hour) and immersion in PVS2 (15 minutes). Encapsulation-vitrification allowed the long-term conservation, and provided high plant material survival rates after cryopreservation of Hancornia speciosa sensitive explants. 

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