Chemometrics characterization of volatile changes in processed bayberry juice versus intact fruit during storage by headspace solid‐phase micro‐extraction combined with GC–MS

2020 ◽  
Vol 44 (6) ◽  
Author(s):  
Haitian Fang ◽  
Jianle Chen ◽  
Yuqi Tian ◽  
Yuxuan Liu ◽  
Hehe Li ◽  
...  
Keyword(s):  
Solid Phase ◽  
Solid Phase Micro Extraction ◽  
Bayberry Juice

Separation and Enhanced Detection of Anesthetic Compounds Using Solid Phase Micro-Extraction (SPME)–Raman Spectroscopy

2014 ◽  
Vol 68 (11) ◽  
pp. 1254-1259 ◽  
Author(s):  
Ikechukwu C. Nwaneshiudu ◽  
Chinwe A. Nwaneshiudu ◽  
Daniel T. Schwartz
Keyword(s):  
Raman Spectroscopy ◽  
Partition Coefficient ◽  
Serum Proteins ◽  
Solid Phase ◽  
Direct Detection ◽  
Solid Phase Micro Extraction ◽  
Spectral Ranges ◽  
Low Solubility ◽  
Do So

Polydimethylsiloxane (PDMS)-based solid-phase micro-extraction (SPME) was used along with Raman spectroscopy (RS) to separate and enhance the detection of five anesthetic compounds (halothane, propofol, isoflurane, enflurane, and etomidate) from aqueous and serum phases. Raman signals in the spectral ranges 250–450 cm−1 and 950–1050 cm−1 allowed the unique characterization of all five compounds when extracted into the PDMS phase. The SPME-RS detection of clinically relevant concentrations of aqueous propofol (6.5 μM) and halothane (200 μM) is shown. We quantify the partition coefficient for aqueous halothane in PDMS as log K = 1.9 ± 0.2. Solid-phase micro-extraction of the anesthetics makes their detection possible without the strong autofluorescent interference of serum proteins. Because of low solubility and/or weak Raman scattering, we found it challenging to detect enflurane, isoflurane, and etomidate directly from the aqueous phase, but could we do so with SPME enhancement. These studies show the potential of SPME-RS as a method for the direct detection of anesthetics in blood.

scholarly journals Aromatic compound in different peach cultivars and effect of preservatives on the final aroma of cooked fruits

2014 ◽  
Vol 68 (6) ◽  
pp. 767-779 ◽  
Author(s):  
Mojca Bavcon-Kralj ◽  
Tjasa Jug ◽  
Erika Komel ◽  
Nikita Fajt ◽  
Kristjan Jarni ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Citric Acid ◽  
Solid Phase ◽  
Negative Impact ◽  
Solid Phase Micro Extraction ◽  
Multiple Discriminant Analysis ◽  
Significant Difference ◽  
And Function ◽  
The Impact

In our study, we were used four yellow-fleshed peach cultivars ?Royal Glory?, ?Redhaven", 'Maria Marta' and 'Norman', during two-year period. The characterization of aromatic constituents of investigated cultivars was done using headspace solid phase micro-extraction (HS-SPME). The intention was to make implicit discrimination between cultivars by analysis of components present in all cultivars during two-year period. Also, the impact of added preservatives (Na-benzoate and citric acid) on the final aroma of cooked peaches was studied. The cultivars' differences and the impact of preservatives (Na-benzoate and citric acid) were statistically evaluated. Multiple discriminant analysis of peaches? aromatic profile was used to segregate investigated peach cultivars. Although they were very similar, the cultivars were segregated by two discriminant function, function 1 (which accounted for 56.9% of this peach model) and function 2 (31.7%). The use of preservatives had also an important impact on the aromatic profile of cooked peaches. The statistical analysis indicated that from 57 identified volatiles, 40 volatiles showed statistically significant difference regarding the way of preservation. The main negative impact had Na-benzoate compared to control or samples preserved with citric acid.

Application of solid-phase immune electron microscopy to study physical and stability characteristics of enterically transmitted non-a, non-b hepatitis virus

1988 ◽  
Vol 46 ◽  
pp. 80-81
Author(s):  
Charles D. Humphrey ◽  
E. H. Cook ◽  
Karen A. McCaustland ◽  
Daniel W. Bradley
Keyword(s):  
Electron Microscopy ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Etiologic Agent ◽  
World Health ◽  
Health Concern ◽  
Morphological Identification ◽  
Immune Electron Microscopy

Enterically transmitted non-A, non-B hepatitis (ET-NANBH) is a type of hepatitis which is increasingly becoming a significant world health concern. As with hepatitis A virus (HAV), spread is by the fecal-oral mode of transmission. Until recently, the etiologic agent had not been isolated and identified. We have succeeded in the isolation and preliminary characterization of this virus and demonstrating that this agent can cause hepatic disease and seroconversion in experimental primates. Our characterization of this virus was facilitated by immune (IEM) and solid phase immune electron microscopic (SPIEM) methodologies.Many immune electron microscopy methodologies have been used for morphological identification and characterization of viruses. We have previously reported a highly effective solid phase immune electron microscopy procedure which facilitated identification of hepatitis A virus (HAV) in crude cell culture extracts. More recently we have reported utilization of the method for identification of an etiologic agent responsible for (ET-NANBH).

TEM characterization of Au/Polysilicon interactions

1990 ◽  
Vol 48 (4) ◽  
pp. 650-651
Author(s):  
N. David Theodore ◽  
Leslie H. Allen ◽  
C. Barry Carter ◽  
James W. Mayer
Keyword(s):  
Solid Phase ◽  
Single Crystal Silicon ◽  
Chemical Vapor ◽  
Electrical Contacts ◽  
Silicon Substrates ◽  
Crystal Silicon ◽  
Transmission Electron ◽  
Polysilicon Films ◽  
The Stability

Metal/polysilicon investigations contribute to an understanding of issues relevant to the stability of electrical contacts in semiconductor devices. These investigations also contribute to an understanding of Si lateral solid-phase epitactic growth. Metals such as Au, Al and Ag form eutectics with Si. reactions in these metal/polysilicon systems lead to the formation of large-grain silicon. Of these systems, the Al/polysilicon system has been most extensively studied. In this study, the behavior upon thermal annealing of Au/polysilicon bilayers is investigated using cross-section transmission electron microscopy (XTEM). The unique feature of this system is that silicon grain-growth occurs at particularly low temperatures ∽300°C).Gold/polysilicon bilayers were fabricated on thermally oxidized single-crystal silicon substrates. Lowpressure chemical vapor deposition (LPCVD) at 620°C was used to obtain 100 to 400 nm polysilicon films. The surface of the polysilicon was cleaned with a buffered hydrofluoric acid solution. Gold was then thermally evaporated onto the samples.

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