In vitro antihypertensive and antioxidative properties of alcalase-derived Moringa oleifera seed globulin hydrolysate and its membrane fractions

2019 ◽  
Vol 43 (2) ◽  
pp. e13862 ◽  
Author(s):  
Taiwo Ayodele Aderinola ◽  
Tayo Nathaniel Fagbemi ◽  
Victor Ndigwe Enujiugha ◽  
Adeola Monisola Alashi ◽  
Rotimi Emmanuel Aluko
Keyword(s):  
Moringa Oleifera ◽  
Antioxidative Properties ◽  
Seed Globulin ◽  
Moringa Oleifera Seed

scholarly journals In vitro antihypertensive and antioxidative properties of trypsin-derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions

2018 ◽  
Vol 7 (1) ◽  
pp. 132-138 ◽  
Author(s):  
Taiwo Ayodele Aderinola ◽  
Tayo Nathaniel Fagbemi ◽  
Victor Ndigwe Enujiugha ◽  
Adeola Monisola Alashi ◽  
Rotimi Emmanuel Aluko
Keyword(s):  
Moringa Oleifera ◽  
Antioxidative Properties ◽  
Seed Globulin ◽  
Moringa Oleifera Seed

scholarly journals Application of Molecularly Structured Ben Oil in Gentamicin Entrapped Lipospheres

2019 ◽  
pp. 262-270 ◽  
Author(s):  
Salome A. Chime ◽  
Ikechukwu V. Onyishi ◽  
Ifeoma O. Eze
Keyword(s):  
Particle Size ◽  
Encapsulation Efficiency ◽  
Moringa Oleifera ◽  
Extended Release ◽  
In Vitro Release ◽  
Antimicrobial Studies ◽  
Microbial Inhibition ◽  
Vitro Release ◽  
Moringa Oleifera Seed

Objective: To formulate extended release gentamicin-entrapped lipospheres using natural lipids from Irvingia wombolu (IWF) and Moringa oleifera seed (MO) popularly known as Ben oil. Methods: Different lipid combinations including IWF and Phospholipon 90H (P90H) and IWF and MO were employed in the formulation of lipospheres. The formulations were analysed for particle size, encapsulation efficiency (EE), pH stability and antimicrobial studies amongst other tests. Also the in vitro release properties were studied in Phosphate buffer pH 7.2. Results: High EE of up to 90 % were obtained for the various LM combinations. The pH was stable over 30 days and the formulations showed about 93 % release of gentamicin at 12 h. Lipospheres formulated with MO matrices showed synergism in the microbial inhibition than other formulations. Conclusion: Natural lipids from Irvingia wombolu and Moringa oleifera seed could be used in formulating oral extended release gentamicin lipospheres.

scholarly journals In-Vitro Antibacterial Activity of Processed Moringa Oleifera Seed Extract: Kill-Time Study

2013 ◽  
Vol 4 (1) ◽  
pp. 47-47 ◽  
Author(s):  
Munirat A. Idris ◽  
Suleyman A. Muyibi ◽  
Mohd Ismail Abdul Karim Parveen Jamal ◽  
Hamzah Mohd. Salleh ◽  
Mohammed Saedi Jami
Keyword(s):  
Antibacterial Activity ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
Time Study ◽  
In Vitro Antibacterial Activity ◽  
Moringa Oleifera Seed

scholarly journals Bactericidal and In Vitro Cytotoxicity of Moringa oleifera Seed Extract and Its Elemental Analysis Using Laser-Induced Breakdown Spectroscopy

2020 ◽  
Vol 13 (8) ◽  
pp. 193
Author(s):  
Reem K. Aldakheel ◽  
Suriya Rehman ◽  
Munirah A. Almessiere ◽  
Firdos A. Khan ◽  
Mohammed A. Gondal ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Inhibitory Action ◽  
Moringa Oleifera ◽  
In Vitro Cytotoxicity ◽  
Laser Induced Breakdown Spectroscopy ◽  
Breakdown Spectroscopy ◽  
Laser Induced Breakdown ◽  
Hct 116 ◽  
Moringa Oleifera Seed

In the current study, we present the correlation between the capability of laser-induced breakdown spectroscopy (LIBS) to monitor the elemental compositions of plants and their biological effects. The selected plant, Moringa oleifera, is known to harbor various minerals and vitamins useful for human health and is a potential source for pharmaceutical interventions. From this standpoint, we assessed the antibacterial and in vitro cytotoxicity of the bioactive components present in Moringa oleifera seed (MOS) extract. Detailed elemental analyses of pellets of MOSs were performed via LIBS. Furthermore, the LIBS outcome was validated using gas chromatography–mass spectrometry (GC-MS). The LIBS signal was recorded, and the presence of the essential elements (Na, Ca, Se, K, Mg, Zn, P, S, Fe and Mn) in the MOSs were examined. The bactericidal efficacy of the alcoholic MOS extract was examined against Escherichia coli (E. coli) and Staphylococcus aureus(S. aureus) by agar well diffusion (AWD) assays and scanning electron microscopy (SEM), which depicted greater inhibition against Gram-positive bacteria. The validity and DNA nuclear morphology of human colorectal carcinoma cells (HCT-116) cells were evaluated via an MTT assay and DAPI staining. The MTT assay results manifested a profoundly inhibitory action of MOS extract on HCT116 cell growth. Additionally, MOS extracts produced inhibitory action in colon cancer cells (HCT-116), whereas no inhibitory action was seen using the same concentrations of MOS extract on HEK-293 cells (non-cancerous cells), suggesting that MOS extracts could be non-cytotoxic to normal cells. The antibacterial and anticancer potency of these MOS extracts could be due to the presence of various bioactive chemical complexes, such as ethyl ester and D-allose and hexadecenoic, oleic and palmitic acids, making them an ideal candidate for pharmaceutical research and applications.

In vitro effects of Moringa oleifera seed lectins on Haemonchus contortus in larval and adult stages

2020 ◽  
Vol 218 ◽  
pp. 108004
Author(s):  
Mário L.S. Medeiros ◽  
Robson R.V. Alves ◽  
Benny F. Oliveira ◽  
Thiago H. Napoleão ◽  
Patrícia M.G. Paiva ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Moringa Oleifera ◽  
In Vitro Effects ◽  
Moringa Oleifera Seed

Antihypertensive and Antioxidant Properties of Moringa Oleifera Seed Enzymatic Protein Hydrolysate and Ultrafiltration Fractions

2019 ◽  
Vol 17 (4) ◽  
pp. 437-444 ◽  
Author(s):  
Aderinola Taiwo Ayodele ◽  
Alashi Adeola Monisola ◽  
Nwachukwu Ifeanyi Daniel ◽  
Fagbemi Tayo Nathaniel ◽  
Enujiugha Victor Ndigwe ◽  
...  
Keyword(s):  
Metal Ion ◽  
Moringa Oleifera ◽  
Membrane Separation ◽  
Protein Hydrolysate ◽  
Antioxidant Properties ◽  
Negative Control ◽  
Antioxidant Power ◽  
Peptide Fraction ◽  
Moringa Oleifera Seed

The aim of this study was to evaluate the in vitro antioxidant and antihypertensive as well as potential cardioprotective properties of peptides generated from enzymatic hydrolysis of Moringa oleifera seed. Moringa protein isolate was digested with alcalase and the hydrolysate fractionated by ultrafiltration using 1, 3, 5, and 10 kDa molecular weight cut-off membranes. Results showed that membrane separation led to decreased free radical (DPPH, hydroxyl) scavenging but enhanced ferric reducing antioxidant power and metal ion chelation properties. The 1–3 kDa peptide fraction was the most active against angiotensin converting enzyme and renin with 6.70% and 24.62% increases, respectively when compared to the hydrolysate. Oral administration (200 mg/kg body weight) to spontaneously hypertensive rats (SHR) resulted in significant decreases in systolic (SBP), diastolic and mean arterial (MAP) blood pressure for the hydrolysates and peptide fractions when compared to the negative control. The 1–3 kDa peptide fraction also showed the greatest (–35 mm Hg) in SBP along with fastest (2 h) reduction of SBP and MAP in the SHR. The hydrolysate produced the most persistent SBP reduction with up to –34 mm Hg after 24 h. However, the longer peptides (>10 kDa) were the most effective (–78 beats per min) in reducing SHR heart rate. The results suggest that the alcalase-hydrolyzed M. oleifera seed peptides could function as potential ingredients for the formulations of functional foods and nutraceuticals with antihypertensive benefits.

In vitro digestibility, structural and functional properties of Moringa oleifera seed proteins

2020 ◽  
Vol 101 ◽  
pp. 105574 ◽  
Author(s):  
Taiwo A. Aderinola ◽  
Adeola M. Alashi ◽  
Ifeanyi D. Nwachukwu ◽  
Tayo N. Fagbemi ◽  
Victor N. Enujiugha ◽  
...  
Keyword(s):  
Functional Properties ◽  
Moringa Oleifera ◽  
Seed Proteins ◽  
In Vitro Digestibility ◽  
Structural And Functional Properties ◽  
Moringa Oleifera Seed

scholarly journals 70 Effects of the addition of Moringa oleifera seed extract to in vitro maturation of bovine oocyte media and its antimicrobial activity

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 3-3
Author(s):  
Theisy P Acosta Pérez
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Maturation ◽  
Moringa Oleifera ◽  
Cumulus Cells ◽  
Seed Extract ◽  
Chi Square ◽  
Cumulus Expansion ◽  
Conventional Antibiotics ◽  
Moringa Oleifera Seed

Abstract Moringa oleifera seed extract was added to the in vitro maturation media of bovine oocytes as an alternative antibiotic to evaluate its effect on the oocyte maturation and its antimicrobial activity. Oocytes (n = 405) were recovered from ovaries from slaughtered cows, classified by quality and divided in four categories according to the cytoplasm appearance and cumulus cells layers. Oocytes were washed four times in TCM-199 supplemented with fetal bovine serum (FBS) and FSH, then placed in maturation media, which contained TCM-199 supplemented with FBS, FSH and the corresponding treatments: TCM-199+gentamicin (Control), TCM-199 without antibiotic (Test), moringa (M) 1.0, 5.0 and 10.0 mg/mL. After 22 h at 38.5°C in a 5% CO2 humidified atmosphere, oocytes were examined to determine cumulus expansion (as categorical results yes or no) and cumulus expansion Index (CEI). Additionally, 400 µL of used maturation media was retrieved and stored for the antimicrobial activity tests in agar cultures. Evaluation of the cultures for colony-forming units (CFU) was done at 24 and 48 h post culture. Results were analyzed with Chi Square test (for expansion rates) and an ANOVA (for the CEI and CFU) using the SAS system, data are presented as mean ± standard error. Statistically, there was no difference between treatments (P >0.05). Numerically M 1.0 and Test obtained the highest maturation rate (96.43%; 96.20%). M 1.0 and Control showed the highest CEI (2.26±0.21; 2.25±0.26). For the CFU count, Control showed the lowest result at 24 and 48 h post culture (0.00 ± 0.00; 0.11 ± 0.33), as M 10.0 showed the highest result at both 24 and 48 h (2.00 ± 3.60; 2.55 ± 4.58). The addition M 1.0 mg/mL to the maturation media is an alternative for the use of conventional antibiotics without decreasing maturation rates.

Evaluation of the In Vivo Acute Toxicity and In Vitro Hemolytic and Immunomodulatory Activities of the Moringa oleifera Flower Trypsin Inhibitor (MoFTI)

2020 ◽  
Vol 27 ◽  
Author(s):  
Leydianne Leite de Siqueira Patriota ◽  
Dayane Kelly Dias do Nascimento Santos ◽  
Bárbara Rafaela da Silva Barros ◽  
Lethícia Maria de Souza Aguiar ◽  
Yasmym Araújo Silva ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Trypsin Inhibitor ◽  
Hemolytic Activity ◽  
Moringa Oleifera ◽  
Biological Activities ◽  
Hematological Parameters ◽  
Behavioral Alterations ◽  
Female Mice

Background: Protease inhibitors have been isolated from plants and present several biological activities, including immunomod-ulatory action. Objective: This work aimed to evaluate a Moringa oleifera flower trypsin inhibitor (MoFTI) for acute toxicity in mice, hemolytic activity on mice erythrocytes and immunomodulatory effects on mice splenocytes. Methods: The acute toxicity was evaluated using Swiss female mice that received a single dose of the vehicle control or MoFTI (300 mg/kg, i.p.). Behavioral alterations were observed 15–240 min after administration, and survival, weight gain, and water and food consumption were analyzed daily. Organ weights and hematological parameters were analyzed after 14 days. Hemolytic activity of MoFTI was tested using Swiss female mice erythrocytes. Splenocytes obtained from BALB/c mice were cultured in the absence or presence of MoFTI for the evaluation of cell viability and proliferation. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) levels were also determined. Furthermore, the culture supernatants were analyzed for the presence of cytokines and nitric oxide (NO). Results: MoFTI did not cause death or any adverse effects on the mice except for abdominal contortions at 15–30 min after administration. MoFTI did not exhibit a significant hemolytic effect. In addition, MoFTI did not induce apoptosis or necrosis in splenocytes and had no effect on cell proliferation. Increases in cytosolic and mitochondrial ROS release, as well as ΔΨm reduction, were observed in MoFTI-treated cells. MoFTI was observed to induce TNF-α, IFN-γ, IL-6, IL-10, and NO release. Conclusion: These results contribute to the ongoing evaluation of the antitumor potential of MoFTI and its effects on other immunological targets.

Evaluation of Moringa oleifera Seed Lectin as a Metal Remover in Aqueous Solutions

2016 ◽  
Vol 23 (7) ◽  
pp. 645-649 ◽  
Author(s):  
José H.E.S. Freitas ◽  
Keissy V. de Santana ◽  
Pollyanna M. da Silva ◽  
Maiara C. de Moura ◽  
Luana C.B.B. Coelho ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Moringa Oleifera ◽  
Moringa Oleifera Seed
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