Characterization of a novel Yersinia ruckeri serotype O1‐specific bacteriophage with virulence‐neutralizing activity

2019 ◽  
Vol 43 (2) ◽  
pp. 285-293 ◽  
Author(s):  
Timothy J. Welch
Keyword(s):  
Yersinia Ruckeri ◽  
Neutralizing Activity ◽  
Specific Bacteriophage

A further Characterization of Yersinia ruckeri (Enteric Redmouth Bacterium)

1979 ◽  
Vol 14 (2) ◽  
pp. 71-78 ◽  
Author(s):  
P.J. O'LEARY ◽  
J.S. ROHOVEC ◽  
J.L. FRYER
Keyword(s):  
Yersinia Ruckeri

scholarly journals Characterization of a plant-produced recombinant human secretory IgA with broad neutralizing activity against HIV

mAbs ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 1585-1597 ◽  
Author(s):  
Matthew Paul ◽  
Rajko Reljic ◽  
Katja Klein ◽  
Pascal MW Drake ◽  
Craig van Dolleweerd ◽  
...  
Keyword(s):  
Secretory Iga ◽  
Neutralizing Activity

Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria

2013 ◽  
Vol 59 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Chao Gong ◽  
Spencer Heringa ◽  
Randhir Singh ◽  
Jinkyung Kim ◽  
Xiuping Jiang
Keyword(s):  
Hydrogen Sulfide ◽  
Lag Phase ◽  
Restriction Enzyme Digestion ◽  
Isolation And Characterization ◽  
The Family ◽  
Processing Plants ◽  
Specific Bacteriophage ◽  
Myoviridae Family ◽  
Restriction Enzyme Digestion Analysis

The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined. Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family. Restriction enzyme digestion analysis with endonuclease DraI detected 6 distinguished patterns among the 9 phages. Phage treatment prevented the growth of SPB for up to 10 h with multiplicity of infection ratios of 1, 10, 100, and 1000 in tryptic soy broth at 30 °C, and extended the lag phase of SPB growth for 2 h at 22 °C with multiplicities of infection of 10, 100, and 1000. These results suggest that the selected bacteriophage cocktail has a high potential for phage application to control SPB in raw animal materials destined for the rendering process.

scholarly journals Analysis of a Highly Flexible Conformational Immunogenic Domain A in Hepatitis C Virus E2

2005 ◽  
Vol 79 (21) ◽  
pp. 13199-13208 ◽  
Author(s):  
Zhen-Yong Keck ◽  
Ta-Kai Li ◽  
Jinming Xia ◽  
Birke Bartosch ◽  
François-Loïc Cosset ◽  
...  
Keyword(s):  
Hepatitis C ◽  
Binding Affinity ◽  
Structural Changes ◽  
Low Ph ◽  
Antibody Binding ◽  
Spatial Proximity ◽  
Neutralizing Activity ◽  
Isolation And Characterization ◽  
Structural And Functional Properties

ABSTRACT Hepatitis C (HCV) E2 glycoprotein is involved in virus attachment and entry, and its structural organization is largely unknown. Characterization of a panel of human monoclonal antibodies (HMAbs) to HCV by competition studies has led to an immunogenic organization model of E2 with three domains designated A, B, and C and epitopes in each domain having similar structural and functional properties. Domain A contains nonneutralizing epitopes, and domains B and C contain neutralizing epitopes. The isolation and characterization of three new HMAbs within domain A for a total of six provide support for this model. All six domain A HMAbs do not neutralize HCV retroviral pseudotype particle (HCVpp) infection on Huh-7 cells, and all six HMAbs have similar binding affinity and maximum binding, B max, a relative indicator of epitope density, as other neutralizing HMAbs, suggesting that neutralization is epitope specific and not by binding to any surface epitope. The dose-dependent neutralizing activity of CBH-7, an HMAb to a domain C epitope in spatial proximity to domain A, and of CBH-5, a domain B HMAb to a more distant epitope, were tested in the presence and absence of each domain A HMAb. No enhancement or reduction in CBH-7 or CBH-5 neutralizing activity was observed, indicating that the potential induction of nonneutralizing antibodies should not be a central issue for HCV vaccine design. To assess whether domain A is involved in the structural changes as part of a pH-dependent virus envelope fusion process, changes in antibody binding patterns to normal pH and acid pH-treated HCVpp were measured. Antibody binding affinity of HMAbs to HCVpp was not affected by low pH. However, the B max values for low-pH-treated HCVpp with antibodies to domain A increased 46%, for domain C (CBH-7) they increased 23%, and for domain B (CBH-5) there was a decrease of 12%. Collectively, the organization and function of HCV E2 antigenic domains are roughly analogous to the large envelope glycoprotein E organizational structure for other flaviviruses with three distinct structural and functional domains.

scholarly journals The characterization of a humanized rabbit anti-TNFα monoclonal antibody

2019 ◽  
Author(s):  
Chao Wang ◽  
Xianpu Ni ◽  
Ying Liu ◽  
Zheng Jin ◽  
Huanzhang Xia
Keyword(s):  
Specific Binding ◽  
Positive Control ◽  
Negative Control ◽  
Necrosis Factor Alpha ◽  
Neutralizing Activity ◽  
Immune Mediated ◽  
Factor Alpha ◽  
Dose Dependent

Tumor necrosis factor alpha (TNFα) is now regarding as a key role in the pathogenesis of immune-mediated disease such as Rheumatoid arthritis (RA), Crohn's Disease, Psoriatic arthritis and Plaque Psoriasis. HERE we have successfully developed an anti-hTNFα monoclonal rabbit antibody(HZ3M) with high binding and neutralizing activity based on RabMAbs platform. Rabbit hybridomas, immunized subcutanrously with 0.4 mg human TNFα, were generated from the rabbit splenocytes and a total of 142 hybridoma clones with specific binding to human TNFa were obtained. The anti-TNFa RabMAbs showed better neutralizing activity and higher antigen binding affinity compared to Humira and Remicade, the elimination phase half-life 58.2h respectively. In vivo efficay studies, normal mice or human TNF-alpha transgenic mice were injected with 1.0 mg/kg Humira (positive control), HZ3M at 0.33?1.0 or 3.0 mg/kg, or solvent (negative control), showed that HZ3M is able to bind and neutralize hTNFα in transgenic and normal mice as well as normal rabbits.Clearly dose-dependent response can be determined. Compared to marketed anti-TNFa drug Humira, the efficacy of HZ3M is seems to show significant longer holding time.Our observations indicate that the HZ3M derived from RabMAb preclinical safety study, and might have a therapeutic role in RA treatment.

Characterization of Xanthomonas axonopodis pv. punicae (Hingorani and Singh) Vauterin et al. and Isolation of Xap Specific Bacteriophage

2018 ◽  
Vol 105 (4-6) ◽  
pp. 210
Author(s):  
K.N. Harshitha ◽  
S.K. Manoranjitham ◽  
E. Somasundaram ◽  
L. Rajendran ◽  
G. Karthikeyan
Keyword(s):  
Xanthomonas Axonopodis ◽  
Specific Bacteriophage
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