Characterization of Xanthomonas axonopodis pv. punicae (Hingorani and Singh) Vauterin et al. and Isolation of Xap Specific Bacteriophage

2018 ◽  
Vol 105 (4-6) ◽  
pp. 210
Author(s):  
K.N. Harshitha ◽  
S.K. Manoranjitham ◽  
E. Somasundaram ◽  
L. Rajendran ◽  
G. Karthikeyan
Keyword(s):  
Xanthomonas Axonopodis ◽  
Specific Bacteriophage

scholarly journals Characterization of Xanthomonas axonopodis pv. phaseoli isolates

2008 ◽  
Vol 34 (3) ◽  
pp. 228-231 ◽  
Author(s):  
Willian Mário de Carvalho Nunes ◽  
Maria Júlia Corazza ◽  
Silvana Aparecida Crestes Dias de Souza ◽  
Siu Mui Tsai ◽  
Eiko Eurya Kuramae
Keyword(s):  
Xanthomonas Campestris ◽  
Random Amplified Polymorphic Dna ◽  
Hypersensitive Reaction ◽  
Chain Reaction ◽  
Xanthomonas Axonopodis ◽  
Paulo State ◽  
Total Dna ◽  
Polymerase Chain ◽  
Bacterial Plant Pathogen

A simple, quick and easy protocol was standardized for extraction of total DNA of the bacteria Xanthomonas axonopodis pv. phaseoli. The DNA obtained by this method had high quality and the quantity was enough for the Random Amplified Polymorphic DNA (RAPD) reactions with random primers, and Polymerase Chain Reaction (PCR) with primers of the hypersensitivity and pathogenicity gene (hrp). The DNA obtained was free of contamination by proteins or carbohydrates. The ratio 260nm/380nm of the DNA extracted ranged from 1.7 to 1.8. The hrp gene cluster is required by bacterial plant pathogen to produce symptoms on susceptible hosts and hypersensitive reaction on resistant hosts. This gene has been found in different bacteria as well as in Xanthomonas campestris pv. vesicatoria (9). The primers RST21 and RST22 (9) were used to amplify the hrp gene of nine different isolates of Xanthomonas axonopodis pv. phaseoli from Botucatu, São Paulo State, Brazil, and one isolate, "Davis". PCR amplified products were obtained in all isolates pathogenic to beans.

scholarly journals Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia

2000 ◽  
Vol 49 (6) ◽  
pp. 680-687 ◽  
Author(s):  
S. Restrepo ◽  
M. C. Duque ◽  
V. Verdier
Keyword(s):  
Xanthomonas Axonopodis

scholarly journals Characterization of ISXax1, a Novel Insertion Sequence Restricted to Xanthomonas axonopodis pv. phaseoli (Variants fuscans and non-fuscans) and Xanthomonas axonopodis pv. vesicatoria

2007 ◽  
Vol 73 (5) ◽  
pp. 1678-1682 ◽  
Author(s):  
Seyed Mehdi Alavi ◽  
Stéphane Poussier ◽  
Charles Manceau
Keyword(s):  
Southern Blot ◽  
Insertion Sequence ◽  
Dot Blot ◽  
Xanthomonas Axonopodis ◽  
High Degree

ABSTRACT ISXax1 is a novel insertion sequence belonging to the IS256 and Mutator families. Dot blot, Southern blot, and PCR analyses revealed that ISXax1 is restricted to Xanthomonas axonopodis pv. phaseoli (variants fuscans and non-fuscans) and X. axonopodis pv. vesicatoria strains. Directed AFLP also showed that a high degree of polymorphism is associated with ISXax1 insertion in these strains.

Isolation and characterization of bacteriophages specific to hydrogen-sulfide-producing bacteria

2013 ◽  
Vol 59 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Chao Gong ◽  
Spencer Heringa ◽  
Randhir Singh ◽  
Jinkyung Kim ◽  
Xiuping Jiang
Keyword(s):  
Hydrogen Sulfide ◽  
Lag Phase ◽  
Restriction Enzyme Digestion ◽  
Isolation And Characterization ◽  
The Family ◽  
Processing Plants ◽  
Specific Bacteriophage ◽  
Myoviridae Family ◽  
Restriction Enzyme Digestion Analysis

The objectives of this study were to isolate and characterize bacteriophages specific to hydrogen-sulfide-producing bacteria (SPB) from raw animal materials, and to develop a SPB-specific bacteriophage cocktail for rendering application. Meat, chicken offal, and feather samples collected from local supermarkets and rendering processing plants were used to isolate SPB (n = 142). Bacteriophages (n = 52) specific to SPB were isolated and purified from the above samples using 18 of those isolated SPB strains as hosts. The host ranges of bacteriophages against 5 selected SPB strains (Escherichia coli, Citrobacter freundii, and Hafnia alvei) were determined. Electron microscopy observation of 9 phages selected for the phage cocktail revealed that 6 phages belonged to the family of Siphoviridae and 3 belonged to the Myoviridae family. Restriction enzyme digestion analysis with endonuclease DraI detected 6 distinguished patterns among the 9 phages. Phage treatment prevented the growth of SPB for up to 10 h with multiplicity of infection ratios of 1, 10, 100, and 1000 in tryptic soy broth at 30 °C, and extended the lag phase of SPB growth for 2 h at 22 °C with multiplicities of infection of 10, 100, and 1000. These results suggest that the selected bacteriophage cocktail has a high potential for phage application to control SPB in raw animal materials destined for the rendering process.

Cloning and characterization of three hypothetical secretion chaperone proteins from Xanthomonas axonopodis pv. citri

2007 ◽  
Vol 53 (2) ◽  
pp. 363-369 ◽  
Author(s):  
Ljubica Tasic ◽  
Paula F.L. Borin ◽  
Letı´cia Khater ◽  
Carlos H.I. Ramos
Keyword(s):  
Chaperone Proteins ◽  
Xanthomonas Axonopodis ◽  
Secretion Chaperone
Sign in / Sign up

Export Citation Format

Share Document