Development of a VP38 recombinant protein-based indirect ELISA for detection of antibodies against grass carp reovirus genotype II (iELISA for detection of antibodies against GCRV II)

2018 ◽  
Vol 41 (12) ◽  
pp. 1811-1819 ◽  
Author(s):  
Weiwei Zeng ◽  
Yingying Wang ◽  
Yanmin Guo ◽  
Sven M. Bergmann ◽  
Jiyuan Yin ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Grass Carp ◽  
Indirect Elisa ◽  
Grass Carp Reovirus ◽  
Genotype Ii

Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II

2019 ◽  
Vol 136 ◽  
pp. 103715 ◽  
Author(s):  
Shixu Liu ◽  
Yingying Wang ◽  
Jiaming Chen ◽  
Qing Wang ◽  
Ouqin Chang ◽  
...  
Keyword(s):  
Cell Line ◽  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow ◽  
A Cell ◽  
Genotype Ii

Comparison of the blood parameters and histopathology between grass carp infected with a virulent and avirulent isolates of genotype II grass carp reovirus

2020 ◽  
Vol 139 ◽  
pp. 103859 ◽  
Author(s):  
Yafang Tang ◽  
Weiwei Zeng ◽  
Yingying Wang ◽  
Qing Wang ◽  
Jiyuan Yin ◽  
...  
Keyword(s):  
Grass Carp ◽  
Blood Parameters ◽  
Grass Carp Reovirus ◽  
Genotype Ii

scholarly journals Recombinant Baculovirus-Produced Grass Carp Reovirus Virus-Like Particles as Vaccine Candidate That Provides Protective Immunity against GCRV Genotype II Infection in Grass Carp

Vaccines ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 53
Author(s):  
Ting Gao ◽  
Caixia Gao ◽  
Siyu Wu ◽  
Yingying Wang ◽  
Jiyuan Yin ◽  
...  
Keyword(s):  
Grass Carp ◽  
Vaccine Development ◽  
Expression System ◽  
Recombinant Baculovirus ◽  
Ctenopharyngodon Idella ◽  
Economic Losses ◽  
Hemorrhagic Disease ◽  
Grass Carp Reovirus ◽  
Virus Like Particles ◽  
Genotype Ii

Grass carp reovirus (GCRV) leads to severe hemorrhagic disease in grass carp (Ctenopharyngodon idella) and causes economic losses in grass carp aquaculture. Recent epidemiological investigations showed that GCRV genotype II is the dominant subtype in China. Therefore, it is very important to develop a novel vaccine for preventing diseases caused by GCRV genotype II. In this study, we employed a bac-to-bac expression system to generate GCRV-II-based virus-like particles (VLPs). Previous studies have shown that the structural proteins VP3, VP4, and VP38 encoded by the segments S3, S6, and S10 of type II GCRV are immunogenic. Hence, the GCRV-VLPs were produced by co-infection of sf9 cells with recombinant baculoviruses PFBH-VP3, PFBH-VP4, and PFBH-VP38. The expressions of VP3, VP4, and VP38 proteins in GCRV-VLPs were tested by IFA and Western blot analysis. By electron microscopic observations of ultrathin sections, purified VLPs showed that the expressed proteins are similar in shape to GCRV genotype II with a size range from 40 nm to 60 nm. The immunogenicity of GCRV-VLPs was evaluated by the injection immunization of grass carp. The analysis of serum-specific IgM antibody showed that grass carp immunized with GCRV-VLPs produced GCRV-specific antibodies. Furthermore, injection with GCRV-VLPs increased the expressions of immune-related genes (IgM, IFN, TLR3, TLR7) in the spleen and kidney. In addition, grass carp immunized with a GCRV-VLPs-based vaccine showed a relative percent survival rate (RPS) of 83.33% after challenge. The data in this study showed that GCRV-VLPs demonstrated an excellent immunogenicity and represent a promising approach for vaccine development against GCRV genotype II infection.

Establishment of a cell line from swim bladder of the Grass carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II

2021 ◽  
Vol 151 ◽  
pp. 104739
Author(s):  
Yuru Yang ◽  
Yingying Wang ◽  
Qing Wang ◽  
Weiwei Zeng ◽  
Yingying Li ◽  
...  
Keyword(s):  
Cell Line ◽  
Grass Carp ◽  
Swim Bladder ◽  
Ctenopharyngodon Idellus ◽  
Grass Carp Reovirus ◽  
A Cell ◽  
Genotype Ii

Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736133
Author(s):  
Jiaming Chen ◽  
Ouqin Chang ◽  
Yingying Li ◽  
Yingying Wang ◽  
Chao Liu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Disease Model ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow ◽  
Genotype Ii
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