Establishment of a cell line from egg of rare minnow Gobiocypris rarus for propagation of grass carp reovirus genotype II

2019 ◽  
Vol 136 ◽  
pp. 103715 ◽  
Author(s):  
Shixu Liu ◽  
Yingying Wang ◽  
Jiaming Chen ◽  
Qing Wang ◽  
Ouqin Chang ◽  
...  
Keyword(s):  
Cell Line ◽  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow ◽  
A Cell ◽  
Genotype Ii

Establishment of a cell line from swim bladder of the Grass carp (Ctenopharyngodon idellus) for propagation of Grass Carp Reovirus Genotype II

2021 ◽  
Vol 151 ◽  
pp. 104739
Author(s):  
Yuru Yang ◽  
Yingying Wang ◽  
Qing Wang ◽  
Weiwei Zeng ◽  
Yingying Li ◽  
...  
Keyword(s):  
Cell Line ◽  
Grass Carp ◽  
Swim Bladder ◽  
Ctenopharyngodon Idellus ◽  
Grass Carp Reovirus ◽  
A Cell ◽  
Genotype Ii

Establishment of a rare minnow (Gobiocypris rarus) disease model for grass carp reovirus genotype II

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736133
Author(s):  
Jiaming Chen ◽  
Ouqin Chang ◽  
Yingying Li ◽  
Yingying Wang ◽  
Chao Liu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Disease Model ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow ◽  
Genotype Ii

RESISTANCE OF SIGCRV TRANSGENIC RARE MINNOW(GOBIOCYPRIS RARUS)TO GRASS CARP REOVIRUS

2010 ◽  
Vol 36 (4) ◽  
pp. 837-842 ◽  
Author(s):  
Sha LIAO ◽  
Yun CHEN ◽  
Fu-Kuan DU ◽  
Ya-Ping WANG ◽  
Lan-Jie LIAO ◽  
...  
Keyword(s):  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Transcriptome analysis of rare minnow (Gobiocypris rarus) infected by the grass carp reovirus

2019 ◽  
Vol 89 ◽  
pp. 337-344 ◽  
Author(s):  
Yusheng Lin ◽  
Bing Wang ◽  
Nenghan Wang ◽  
Gang Ouyang ◽  
Hong Cao
Keyword(s):  
Transcriptome Analysis ◽  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Grass carp reovirus activates RNAi pathway in rare minnow, Gobiocypris rarus

Aquaculture ◽  
2009 ◽  
Vol 289 (1-2) ◽  
pp. 1-5 ◽  
Author(s):  
Jianguo Su ◽  
Zuoyan Zhu ◽  
Yaping Wang ◽  
Jun Zou ◽  
Na Wang ◽  
...  
Keyword(s):  
Grass Carp ◽  
Rnai Pathway ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)

2009 ◽  
Vol 26 (6) ◽  
pp. 828-835 ◽  
Author(s):  
Jianguo Su ◽  
Chunrong Yang ◽  
Zuoyan Zhu ◽  
Yaping Wang ◽  
Songhun Jang ◽  
...  
Keyword(s):  
Grass Carp ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Rare Minnow

scholarly journals A Novel Subunit Vaccine Based on Outer Capsid Proteins of Grass Carp Reovirus (GCRV) Provides Protective Immunity against GCRV Infection in Rare Minnow (Gobiocypris rarus)

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 945
Author(s):  
Changyong Mu ◽  
Vikram N. Vakharia ◽  
Yong Zhou ◽  
Nan Jiang ◽  
Wenzhi Liu ◽  
...  
Keyword(s):  
Grass Carp ◽  
Subunit Vaccine ◽  
Capsid Proteins ◽  
Economic Losses ◽  
Hemorrhagic Disease ◽  
Gobiocypris Rarus ◽  
Grass Carp Reovirus ◽  
Expression Levels ◽  
Rare Minnow ◽  
Outer Capsid

The grass carp hemorrhagic disease, caused by the grass carp reovirus (GCRV), has resulted in severe economic losses in the aquaculture industry in China. VP4 and VP35 are outer capsid proteins of GCRV and can induce an immune response in the host. Here, three recombinant baculoviruses, AcMNPV-VP35, AcMNPV-VP4, and AcMNPV-VP35-VP4, were generated to express recombinant VP4 and VP35 proteins from GCRV type II in insect cells by using the Bac-to-Bac baculovirus expression system to create a novel subunit vaccine. The expression of recombinant VP35, VP4, and VP35-VP4 proteins in Sf-9 cells were confirmed by Western blotting and immunofluorescence. Recombinant VP35, VP4, and VP35-VP4 were purified from baculovirus-infected cell lysates and injected intraperitoneally (3 μg/fish) into the model rare minnow, Gobiocypris rarus. After 21 days, the immunized fish were challenged with virulent GCRV. Liver, spleen, and kidney samples were collected at different time intervals to evaluate the protective efficacy of the subunit vaccines. The mRNA expression levels of some immune-related genes detected by using quantitative real-time PCR (qRT-PCR) were significantly upregulated in the liver, spleen, and kidney, with higher expression levels in the VP35-VP4 group. The nonvaccinated fish group showed 100% mortality, whereas the VP35-VP4, VP4, and VP35 groups exhibited 67%, 60%, and 33% survival, respectively. In conclusion, our results revealed that recombinant VP35 and VP4 can induce immunity and protect against GCRV infection, with their combined use providing the best effect. Therefore, VP35 and VP4 proteins can be used as a novel subunit vaccine against GCRV infection.

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