Transmission of lymphocystis disease virus to cultured gilthead seabream, Sparus aurata L., larvae

2012 ◽  
Vol 36 (6) ◽  
pp. 569-576 ◽  
Author(s):  
I Cano ◽  
E J Valverde ◽  
E Garcia-Rosado ◽  
M C Alonso ◽  
B Lopez-Jimena ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

scholarly journals Target organs for lymphocystis disease virus replication in gilthead seabream (Sparus aurata)

2017 ◽  
Vol 48 (1) ◽  
Author(s):  
Estefania J. Valverde ◽  
Juan J. Borrego ◽  
M. Carmen Sarasquete ◽  
Juan B. Ortiz-Delgado ◽  
Dolores Castro
Keyword(s):  
Disease Virus ◽  
Virus Replication ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Target Organs ◽  
Lymphocystis Disease

scholarly journals Antiviral activity of Mx proteins from gilthead seabream (Sparus aurata) against lymphocystis disease virus (LCDV)

2013 ◽  
Vol 34 (6) ◽  
pp. 1651
Author(s):  
M.A. Fernández-Trujillo ◽  
E. García-Rosado ◽  
M.C. Alonso ◽  
J.J. Borrego ◽  
M.C. Álvarez ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Disease Virus ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease

Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique

2006 ◽  
Vol 113 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Cano ◽  
M.C. Alonso ◽  
E. Garcia-Rosado ◽  
S. Rodriguez Saint-Jean ◽  
D. Castro ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immunoblot Technique

scholarly journals Evaluation of Gilthead Seabream (Sparus aurata) Immune Response after LCDV-Sa DNA Vaccination

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1613
Author(s):  
Rocío Leiva-Rebollo ◽  
Dolores Castro ◽  
Patricia Moreno ◽  
Juan J. Borrego ◽  
Alejandro M. Labella
Keyword(s):  
Immune Response ◽  
Neutralizing Antibodies ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Type I ◽  
Protective Capacity ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immune Related Genes ◽  
Innate Antiviral Immunity

Lymphocystis disease is the main viral pathology reported in gilthead seabream. Its etiological agent is Lymphocystis disease virus 3 (LCDV-Sa), genus Lymphocystivirus, family Iridoviridae. There are no effective treatments or vaccines for LCDV control, thus the main aim of this study was to develop a DNA vaccine, and to evaluate both the protection conferred against LCDV-Sa infection and the immune response in vaccinated fish. The vaccine was constructed by cloning the mcp gene (ORF LCDVSa062R) into pcDNA3.1/NT-GFP-TOPO. Two independent vaccination trials were conducted. In the first one, 5–7 g fish were intramuscularly injected with the vaccine (pcDNA-MCP) or the empty-plasmid, and the distribution and expression of the vaccine was investigated. Furthermore, vaccinated fish were challenged with LCDV-Sa in order to access the protective capacity of the vaccine. In the second trial, 70–100 g fish were vaccinated as specified, and the immune response was evaluated analyzing the expression of 23 immune-related genes and the production of specific antibodies. The results showed that the vaccine triggers an immune response characterized by the overexpression of genes relating to the inflammatory process, but not the innate antiviral immunity relating to type I IFN (interferon), and also induces the production of specific neutralizing antibodies, which could explain the protection against LCDV-Sa in vaccinated fish.

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