Genotoxic and antigenotoxic potential of amygdalin on isolated human lymphocytes by the comet assay

2020 ◽  
Vol 44 (10) ◽  
Author(s):  
Esra Erikel ◽  
Deniz Yuzbasioglu ◽  
Fatma Unal
Keyword(s):  
Comet Assay ◽  
Human Lymphocytes ◽  
Isolated Human Lymphocytes

scholarly journals Effects of photopolymerisation on genotoxicity of composite adhesives in the comet assay

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 617-627
Author(s):  
Stefan Dacic ◽  
Ninoslav Djelic ◽  
Milena Radakovic ◽  
Nada Lakic ◽  
Aleksandar Veselinovic ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Lymphocytes ◽  
Negative Control ◽  
In Vivo Studies ◽  
Halogen Lamp ◽  
Genotoxic Effects ◽  
Significant Difference ◽  
Isolated Human Lymphocytes

Certain in vivo studies have shown that the application of adhesives directly onto the open pulp or on a thin layer of dentin causes inflammation and pulpal abscesses. This reaction is related to toxic effects of monomers from adhesives. It has been confirmed that after proper illumination the adhesives become less toxic. The aim of the study was to examine genotoxicity of non-polymerised, partly polymerised and polymerised adhesives on isolated human lymphocytes using the alkaline Comet assay. Adper Single bond2 and Adper Easy One/3M ESPE adhesive photopolymerisation was performed by Elipar Highlight 3M ESPE halogen lamp for 0, 10 and 40 sec, at final concentrations of 100, 200, 500 and 1000 ?g/mL. With both adhesives, photopolymerisation at 0 and 10 seconds showed statistically significant increase in DNA damage in comparision to the negative control (solvent). On the other hand, after 40 seconds of photopolymerisation of both adhesives in all tested concentrations, the degree of DNA damage in Comet assay had no significant difference (P>0.05, ?2 test) compared to the negative control. Therefore, only the 40 seconds of photopolymerisation prevented genotoxic effects of both adhesives in the Comet assay.

DNA damage assessment by comet assay of human lymphocytes exposed to jet propulsion fuels

2002 ◽  
Vol 40 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Shawna M. Jackman ◽  
Geraldine M. Grant ◽  
Christopher J. Kolanko ◽  
David A. Stenger ◽  
Joginder Nath
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Damage Assessment ◽  
Human Lymphocytes ◽  
Jet Propulsion

Genotoxicity of honeybee venom in human lymphocytes using Comet assay

2008 ◽  
Vol 180 ◽  
pp. S104
Author(s):  
Goran Gajski ◽  
Vera Garaj-Vrhovac
Keyword(s):  
Comet Assay ◽  
Human Lymphocytes ◽  
Honeybee Venom

scholarly journals The potential toxic impact of different gadolinium-based contrast agents combined with 7-T MRI on isolated human lymphocytes

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Björn Friebe ◽  
Frank Godenschweger ◽  
Mahsa Fatahi ◽  
Oliver Speck ◽  
Dirk Roggenbuck ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Human Lymphocytes ◽  
Toxic Impact ◽  
Isolated Human Lymphocytes

Imatinib (STI571) Induces DNA Damage in BCR/ABL-Expressing Leukemic Cells but Not in Normal Lymphocytes.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4353-4353
Author(s):  
Janusz Blasiak ◽  
Jozef Drzewoski ◽  
Tomasz Poplawski ◽  
Agnieszka Czechowska
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Human Lymphocytes ◽  
Direct Interaction ◽  
K562 Cells ◽  
Dna Lesions ◽  
Dna Glycosylase ◽  
Leukemic Cells ◽  
Drug Induced ◽  
Strand Breaks

Abstract Imatinib (STI571) is a 2-phenylaminopyrimidine derivative used mostly in the treatment of chronic myeloid leukaemia. It targets specifically the BCR/ABL oncogenic tyrosine kinase, inhibiting its activity. Using the alkaline comet assay we showed that STI571 at concentrations ranging from 0.05 to 2 μM induced DNA damage in human leukemic K562 cells expressing the BCR/ABL oncogene, whereas it had no effect in normal human lymphocytes. Because the extent of DNA damage observed in the neutral and pH 12.1 versions of the comet assay was much lesser than in the alkaline version, we concluded that the drug induced DNA alkali-labile sites rather than strand breaks. Imatinib did not induce DNA strand breaks in the direct interaction with DNA as examined by the plasmid relaxation assay. K562 cells were unable to repair H2O2-induced DNA damage during a 120-min incubation, if they had been preincubated with STI571, whereas normal lymphocytes did so within 60 min. Pre-treatment of K562 cells with vitamins A, C and E reduced the extent of DNA damage evoked by STI571. Similar results brought experiments with the nitrone spin traps POBN and PBN, suggesting that free radicals may be involved in the formation of DNA lesions induced by STI571 in K562 cells. These cells exposed to imatinib and treated with endonuclease III, formamidopyrimidine-DNA glycosylase and 3-methyladenine-DNA glycosylase II, the enzymes recognizing oxidized and alkylated bases, displayed greater extent of DNA damage than those not treated with these enzymes.

Interaction of hypothalamic Na,K-ATPase inhibitor with isolated human peripheral blood mononuclear cells

1994 ◽  
Vol 14 (5) ◽  
pp. 231-242 ◽  
Author(s):  
Beatrice M. Anner ◽  
Danielle Lacotte ◽  
Rolf M. Anner ◽  
Marlis Moosmayer
Keyword(s):  
Cell Viability ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Exchange Process ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Isolated Human Lymphocytes

A ligand for the digitalis receptor located on the membrane-embedded Na,K-ATPase (NKA; EC 3.6.1.37) has been isolated from bovine hypothalamus (hypothalamic inhibitory factor; HIF) and identified as isomeric ouabain (Tymiaket al, 1993,Proc. Natl. Acad. Sci.90: 8189–8193). In analogy to cardioactive steroids (CS) derived from plants or from toad, HIF inhibits the Na/K-exchange process and the ATPase activity of isolated Na,K-ATPase although by a different molecular action mechanism. In the present work we show that, as plant-derived ouabain, HIF inhibits86Rb-uptake by isolated human lymphocytes with an IC50 of about 20 nM; above this concentration HIF reduces cell viability in contrast to ouabain. The decrease in cell viability by excess HIF is accompanied by discrete morphological alterations (mitochondrial swelling) visible by transmission electron microscopy of ultra-thin sectioned peripheral blood mononuclear cells. Taken together the results show that the hypothalamic NKA inhibitor blocks NKA of isolated human lymphocytes with high potency at nanomolar concentrations without toxicity; concentrations exceeding the ones required to block86Rb-uptake reduce cell viability, probably due to leak formation across the NKA molecule. Thus, lymphocytes constitute a potential target for HIF action and by their altered NKA status a possible messenger between the nervous and the immune system.

Sign in / Sign up

Export Citation Format

Share Document