Jujube honey induces apoptosis in human hepatocellular carcinoma HepG2 cell via DNA damage, p53 expression, and caspase activation

2019 ◽  
Vol 43 (11) ◽  
Author(s):  
Ni Cheng ◽  
Haoan Zhao ◽  
Sinan Chen ◽  
Qiong He ◽  
Wei Cao
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Damage ◽  
Hepg2 Cell ◽  
Human Hepatocellular Carcinoma ◽  
Caspase Activation ◽  
P53 Expression

The cyanobacterial oligopeptides microginins induce DNA damage in the human hepatocellular carcinoma (HepG2) cell line

Chemosphere ◽  
2020 ◽  
Vol 240 ◽  
pp. 124880 ◽  
Author(s):  
Andrea Zsuzsanna Ujvárosi ◽  
Klara Hercog ◽  
Milán Riba ◽  
Sándor Gonda ◽  
Metka Filipič ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Damage ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Human Hepatocellular Carcinoma ◽  
Hepg2 Cell Line

ARID2 modulates DNA damage response in human hepatocellular carcinoma cells

2017 ◽  
Vol 66 (5) ◽  
pp. 942-951 ◽  
Author(s):  
Atsushi Oba ◽  
Shu Shimada ◽  
Yoshimitsu Akiyama ◽  
Taketo Nishikawaji ◽  
Kaoru Mogushi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Dna Damage ◽  
Dna Damage Response ◽  
Carcinoma Cells ◽  
Human Hepatocellular Carcinoma ◽  
Hepatocellular Carcinoma Cells ◽  
Damage Response ◽  
Human Hepatocellular Carcinoma Cells

scholarly journals Gene Network Analysis of Glucose Linked Signaling Pathways and Their Role in Human Hepatocellular Carcinoma Cell Growth and Survival in HuH7 and HepG2 Cell Lines

2015 ◽  
Vol 2015 ◽  
pp. 1-19 ◽  
Author(s):  
Emmanuelle Berger ◽  
Nathalie Vega ◽  
Michèle Weiss-Gayet ◽  
Alain Géloën
Keyword(s):  
Hepatocellular Carcinoma ◽  
Signaling Pathways ◽  
Hepg2 Cell ◽  
Cell Lines ◽  
Cancer Progression ◽  
High Glucose ◽  
Gene Network ◽  
Target Genes ◽  
Human Hepatocellular Carcinoma ◽  
Network Analyses

Cancer progression may be affected by metabolism. In this study, we aimed to analyze the effect of glucose on the proliferation and/or survival of human hepatocellular carcinoma (HCC) cells. Human gene datasets regulated by glucose were compared to gene datasets either dysregulated in HCC or regulated by other signaling pathways. Significant numbers of common genes suggested putative involvement in transcriptional regulations by glucose. Real-time proliferation assays using high (4.5 g/L)versuslow (1 g/L) glucose on two human HCC cell lines and specific inhibitors of selected pathways were used for experimental validations. High glucose promoted HuH7 cell proliferation but not that of HepG2 cell line. Gene network analyses suggest that gene transcription by glucose could be mediated at 92% through ChREBP in HepG2 cells, compared to 40% in either other human cells or rodent healthy liver, with alteration of LKB1 (serine/threonine kinase 11) and NOX (NADPH oxidases) signaling pathways and loss of transcriptional regulation of PPARGC1A (peroxisome-proliferator activated receptors gamma coactivator 1) target genes by high glucose. Both PPARA and PPARGC1A regulate transcription of genes commonly regulated by glycolysis, by the antidiabetic agent metformin and by NOX, suggesting their major interplay in the control of HCC progression.

Synthesis, Characterization and Anti-hepatoma Activity of New Hederagenin Derivatives

2020 ◽  
Vol 20 (3) ◽  
pp. 252-257
Author(s):  
Xiao Liu ◽  
Lu Sun ◽  
Qing-Hua Liu ◽  
Bao-Quan Chen ◽  
Yu-Ming Liu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Hepg2 Cell ◽  
Normal Cell ◽  
Biological Significance ◽  
Cancer Cell Line ◽  
Human Hepatocellular Carcinoma ◽  
Hepg2 Cell Line ◽  
Normal Cell Line

Background: Based on the biological significance of hederagenin-type saponins found in our previous investigation, a series of new hederagenin derivatives were designed and synthesized. Methods : Their in vitro antiproliferative activities were evaluated against the HepG2 liver cancer cell line and normal cell line L929 by MTT assay. Results: The preliminary bioassay results demonstrated that all the tested compounds 1-7 showed potent anti-hepatoma activities, and some compounds exhibited better effects than 5-fluorouracil against human hepatocellular carcinoma HepG2 cell line. Furthermore, compound 5 showed a significant antihepatoma activity against HepG2 cells with an IC50 value of 1.88 µM. Besides, all of the tested compounds showed a low cytotoxic effect against the normal cell line L929. Conclusion: All the compounds 1-7 displayed superior selectivity against human hepatocellular carcinoma HepG2 cell line, and the results suggest that the structural modifications of C ring on the hederagenin backbone are vital for modulating anti-hepatoma activities.

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