Spermatogenesis in the inseminating African butterflyfish Pantodon buchholzi (Teleostei: Osteoglossiformes: Pantodontidae) with the revision of residual bodies formation

2020 ◽  
Vol 97 (5) ◽  
pp. 1491-1506
Author(s):  
Anna M. Dymek ◽  
Anna Pecio
Keyword(s):  
Residual Bodies

scholarly journals Ezrin is an Actin Binding Protein That Regulates Sertoli Cell and Spermatid Adhesion During Spermatogenesis

Endocrinology ◽  
2014 ◽  
Vol 155 (10) ◽  
pp. 3981-3995 ◽  
Author(s):  
N. Ece Gungor-Ordueri ◽  
Elizabeth I. Tang ◽  
Ciler Celik-Ozenci ◽  
C. Yan Cheng
Keyword(s):  
Sertoli Cell ◽  
Sertoli Cells ◽  
Adherens Junction ◽  
Actin Binding ◽  
Permeability Barrier ◽  
Tight Junction Permeability ◽  
Residual Bodies ◽  
Cell Interface

Abstract During spermatogenesis, the transport of spermatids and the release of sperms at spermiation and the remodeling of the blood-testis barrier (BTB) in the seminiferous epithelium of rat testes require rapid reorganization of the actin-based cytoskeleton. However, the mechanism(s) and the regulatory molecule(s) remain unexplored. Herein we report findings that unfold the functional significance of ezrin in the organization of the testis-specific adherens junction at the spermatid-Sertoli cell interface called apical ectoplasmic specialization (ES) in the adluminal compartment and the Sertoli cell-cell interface known as basal ES at the BTB. Ezrin is expressed at the basal ES/BTB in all stages, except from late VIII to IX, of the epithelial cycle. Its knockdown by RNA interference (RNAi) in vitro perturbs the Sertoli cell tight junction-permeability barrier via a disruption of the actin microfilaments in Sertoli cells, which in turn impeded basal ES protein (eg, N-cadherin) distribution, perturbing the BTB function. These findings were confirmed by a knockdown study in vivo. However, the expression of ezrin at the apical ES is restricted to stage VIII of the cycle and limited only between step 19 spermatids and Sertoli cells. A knockdown of ezrin in vivo by RNAi was found to impede spermatid transport, causing defects in spermiation in which spermatids were embedded deep inside the epithelium, and associated with a loss of spermatid polarity. Also, ezrin was associated with residual bodies and phagosomes, and its knockdown by RNAi in the testis also impeded the transport of residual bodies/phagosomes from the apical to the basal compartment. In summary, ezrin is involved in regulating actin microfilament organization at the ES in rat testes.

Residual Bodies of Seminiferous Tubules of the Rat

Nature ◽  
1959 ◽  
Vol 184 (4682) ◽  
pp. 249-251 ◽  
Author(s):  
B. V. KINGSLEY SMITH ◽  
D. LACY
Keyword(s):  
Seminiferous Tubules ◽  
Residual Bodies

scholarly journals Mouse testes contain two size classes of actin mRNA that are differentially expressed during spermatogenesis.

1985 ◽  
Vol 5 (7) ◽  
pp. 1649-1654 ◽  
Author(s):  
S H Waters ◽  
R J Distel ◽  
N B Hecht
Keyword(s):  
Untranslated Region ◽  
Cell Types ◽  
Dot Blot ◽  
Size Classes ◽  
Cytoplasmic Actin ◽  
Beta Actin ◽  
Actin Mrna ◽  
Residual Bodies ◽  
Pachytene Spermatocytes ◽  
Sexually Mature

Using several actin isotype-specific cDNA probes, we found actin mRNA of two size classes, 2.1 and 1.5 kilobases (kb), in extracts of polyadenylated and nonpolyadenylated RNA from sexually mature CD-1 mouse testes. Although the 2.1-kb sequence was present in both meiotic and postmeiotic testicular cell types, it decreased manyfold in late haploid cells. The 1.5-kb actin sequence was not detectable in meiotic pachytene spermatocytes (or in liver or kidney cells), but was present in round and elongating spermatids and residual bodies. To differentiate between the beta- and gamma-actin mRNAs, we isolated a cDNA, pMGA, containing the 3' untranslated region of a mouse cytoplasmic actin that has homology to the 3' untranslated region of a human gamma-actin cDNA but not to the 3' untranslated regions of human alpha-, beta-, or cardiac actins. Dot blot hybridizations with pMGA detected high levels of presumptive gamma-actin mRNA in pachytene spermatocytes and round spermatids, with lower amounts found in elongating spermatids. Hybridization with the 3' untranslated region of a rat beta-actin probe revealed that round spermatids contained higher levels of beta-actin mRNA than did pachytene spermatocytes or residual bodies. Both probes hybridized to the 2.1-kb actin mRNA but failed to hybridize to the 1.5-kb mRNA.

Autophagy and Apoptosis in the Midgut Epithelium of Millipedes

2019 ◽  
Vol 25 (4) ◽  
pp. 1004-1016 ◽  
Author(s):  
M.M. Rost-Roszkowska ◽  
J. Vilimová ◽  
K. Tajovský ◽  
A. Chachulska-Żymełka ◽  
A. Sosinka ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscope ◽  
Fluorescence Microscope ◽  
Midgut Epithelium ◽  
Digestive Cells ◽  
Histochemical Methods ◽  
Transmission Electron ◽  
Regenerative Cells ◽  
Residual Bodies

AbstractThe process of autophagy has been detected in the midgut epithelium of four millipede species: Julus scandinavius, Polyxenus lagurus, Archispirostreptus gigas, and Telodeinopus aoutii. It has been examined using transmission electron microscopy (TEM), which enabled differentiation of cells in the midgut epithelium, and some histochemical methods (light microscope and fluorescence microscope). While autophagy appeared in the cytoplasm of digestive, secretory, and regenerative cells in J. scandinavius and A. gigas, in the two other species, T. aoutii and P. lagurus, it was only detected in the digestive cells. Both types of macroautophagy, the selective and nonselective processes, are described using TEM. Phagophore formation appeared as the first step of autophagy. After its blind ends fusion, the autophagosomes were formed. The autophagosomes fused with lysosomes and were transformed into autolysosomes. As the final step of autophagy, the residual bodies were detected. Autophagic structures can be removed from the midgut epithelium via, e.g., atypical exocytosis. Additionally, in P. lagurus and J. scandinavius, it was observed as the neutralization of pathogens such as Rickettsia-like microorganisms. Autophagy and apoptosis ca be analyzed using TEM, while specific histochemical methods may confirm it.

scholarly journals Ultrastructural study of degradation of calcium phosphate ceramic by human monocytes and modulation of this activity by HILDA/LIF cytokine.

1996 ◽  
Vol 44 (10) ◽  
pp. 1131-1140 ◽  
Author(s):  
M D Benahmed ◽  
D Heymann ◽  
M Berreur ◽  
M Cottrel ◽  
A Godard ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Culture Medium ◽  
Marked Decrease ◽  
Calcium Phosphate Ceramic ◽  
Multinuclear Cells ◽  
Residual Bodies ◽  
First Time

Biodegradation of ceramics in vivo is achieved essentially by monocytes and multinuclear cells (osteoclasts). Monocytes are the key element in this process because they intervene first at the biomaterial implantation site during inflammatory reaction. In this work, in vitro studies were conducted on an ultrastructural scale to determine the specific behavior of these cells with regard to a calcium phosphate (CaP) ceramic. Two types of phagocytosis were observed when cells came into contact with the biomaterial: either CaP crystals were taken up alone and then dissolved in the cytoplasm after disappearance of the phagosome membrane or they were incorporated together with large quantities of culture medium, in which case dissolution occurred after the formation of heterophagosomes. Phagocytosis of CaP coincided with autophagy and the accumulation of residual bodies in the cells. Addition of HILDA/LIF factor to these cultures induced a very marked decrease in phagocytotic activity directed at the capture of CaP crystals and culture medium. Autophagy was reduced, and residual bodies were rare or absent. This study specifies the role of monocytes in CaP biodegradation and demonstrates for the first time that HILDA/LIF has a biological effect on this cell line.

Cell degeneration and gill remodelling during basidiocarp development in the fungus Coprinus cinereus

1991 ◽  
Vol 69 (6) ◽  
pp. 1161-1169 ◽  
Author(s):  
Benjamin C. Lu
Keyword(s):  
Hydrolytic Enzymes ◽  
Multivesicular Body ◽  
Coprinus Cinereus ◽  
High Dose ◽  
Residual Body ◽  
Multivesicular Bodies ◽  
Cell Degeneration ◽  
Cellular Debris ◽  
Extensive Cell ◽  
Residual Bodies

The early mushroom gill development in a primordium of Coprinus cinereus was studied by electron microscopy. Extensive cell degeneration and cell death were found in gill cavities but not within gill domains. These degenerative cells were found to contain multivesicular and membranous residual bodies, suggesting that the multivesicular bodies are part of the cell degeneration. Cellular debris was observed in intercellular spaces probably as a consequence of cell lysis. The presence of multivesicular bodies was also observed in cells shortly before Coprinus basidiocarps underwent autolysis: a high dose of hydrolytic enzymes can be extracted from such basidiocarps. The high numbers of multivesicular bodies, the membranous residual bodies, and the cellular debris in the primordial tissues are manifestations of cell degeneration that may be a prerequisite to gill remodelling during early primordial development. Key words: cell degeneration, gill remodelling, multivesicular body, residual body, mushroom development.

The fine structure of Verson's glands in molting larvae of Calpodes ethlius (Hesperiidae, Lepidoptera)

1973 ◽  
Vol 51 (11) ◽  
pp. 1201-1210 ◽  
Author(s):  
Joan Lai-Fook
Keyword(s):  
Fine Structure ◽  
Secretory Cell ◽  
Duct Cell ◽  
Secretory Cells ◽  
Dense Granules ◽  
Cell Processes ◽  
Residual Bodies

The three cells which make up Verson's glands in Calpodes undergo drastic changes as they produce the cuticular linings and the secretions of the glands. The duct cell secretes only the typical cuticular duct. The saccule cell produces both the atypical cuticular saccule and dense granules which are discharged just before ecdysis. The secretory cell is much enlarged by vacuoles which remain separate until they too are discharged before ecdysis. Dense granules are also produced by the secretory cell. During deposition of the cuticular duct and saccule, their lumina arc packed with cell processes containing microtubules, which appear to arise from centrioles. Isolation and residual bodies appear in both the saccule and secretory cells even before discharge of their secretions.

Sign in / Sign up

Export Citation Format

Share Document