Host suitability and feeding preference of the African citrus triozid Trioza erytreae Del Guercio (Hemiptera: Triozidae), natural vector of “Candidatus Liberibacter africanus”

2018 ◽  
Vol 143 (3) ◽  
pp. 262-270 ◽  
Author(s):  
Owusu Fordjour Aidoo ◽  
Chrysantus Mbi Tanga ◽  
Fathiya Mbarak Khamis ◽  
Brenda Amondi Rasowo ◽  
Samira Abuelgasim Mohamed ◽  
...  
Keyword(s):  
Feeding Preference ◽  
Host Suitability ◽  
Trioza Erytreae ◽  
Natural Vector ◽  
Candidatus Liberibacter

scholarly journals Feeding and Oviposition Behaviour of Trioza erytreae (Hemiptera: Triozidae) on Different Citrus Rootstock Material Available in Europe

Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 623
Author(s):  
Estrella Hernández-Suárez ◽  
Laura Suárez-Méndez ◽  
Moneyba Parrilla ◽  
Juan M. Arjona-López ◽  
Aurea Hervalejo ◽  
...  
Keyword(s):  
Feeding Preference ◽  
Poncirus Trifoliata ◽  
Choice Trial ◽  
Carrizo Citrange ◽  
Trioza Erytreae ◽  
Citrus Rootstock ◽  
Candidatus Liberibacter ◽  
Citrus Macrophylla ◽  
Insect Reproduction ◽  
Cleopatra Mandarin

Trioza erytreae (Del Guercio, 1918) (Hemiptera: Triozidae) is a vector of Candidatus Liberibacter spp., the causal agent of Huanglongbing disease (HLB). This study evaluates the preference of T. erytreae in different citrus seedlings. Thus, six different non-grafted citrus rootstocks were used for these experiments: (a) Carrizo citrange; (b) Citrus macrophylla; (c) ‘Cleopatra’ mandarin; (d) Forner-Alcaide No. 5; (e) Forner-Alcaide No. 517, and (f) Poncirus trifoliata (‘Flying Dragon’). The behaviour and survival of this psyllid was evaluated through the feeding preference of T. erytreae adults for different rootstocks (in a choice trial under greenhouse conditions) and oviposition and survival of T. erytreae adults on the different citrus material (in a no-choice trial under laboratory conditions). Trioza erytreae showed a clear preference for hosting and feeding on C. macrophylla, and Carrizo citrange was the most suitable rootstock for insect reproduction and survival followed by C. macrophylla. Conversely, Poncirus trifoliata was the least attractive rootstock to T. erytreae adults in the greenhouse trial and led to significantly lower T. erytreae survival. Our results suggest that conventional citrus rootstocks, such as Carrizo citrange and C. macrophylla, could increase T. erytreae populations.

scholarly journals Mitochondrial genetic variation reveals phylogeographic structure and cryptic diversity in Trioza erytreae

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Inusa Ajene ◽  
Fathiya M. Khamis ◽  
Gerhard Pietersen ◽  
Barbara van Asch
Keyword(s):  
South Africa ◽  
Citrus Greening ◽  
Phylogeographic Structure ◽  
Citrus Greening Disease ◽  
Trioza Erytreae ◽  
Baseline Information ◽  
Candidatus Liberibacter ◽  
Mitochondrial Sequences ◽  
Greening Disease ◽  
Genetic Clustering

AbstractTrioza erytreae is the main vector for ‘Candidatus Liberibacter africanus’, the causative agent of African Citrus Greening disease. The insect is widespread in Africa, and has recently disseminated to Southwestern Europe. This study aimed at generating reference mitogenome sequences for T. erytreae, as a background for future genetic diversity surveys. Complete mitochondrial sequences of three specimens collected in Ethiopia, Uganda and South Africa were recovered using Ion Torrent technology. The mitogenomes of T. erytreae from Uganda and Ethiopia were highly similar, and distinct from that found in South Africa. The phylogeographic structure of T. erytreae was assessed using genetic clustering and pairwise distances, based on a dataset of public COI sequences recorded as T. erytreae. The dataset revealed ten haplotypes with strong phylogeographic structure in Africa and Europe. Three haplotypes found in Kenya on Clausena anisata belonged to pairs separated by distances as high as 11.2%, and were basal to all other sequences. These results indicate that not all sequences identified as T. erytreae belong to the same species, and that some degree of specificity with different plant hosts is likely to exist. This study provides new baseline information on the diversity of T. erytreae, with potential implications for the epidemiology of African Citrus Greening disease.

scholarly journals ‘Candidatus Liberibacter Solanacearum’ Is Unlikely to Be Transmitted Spontaneously from Infected Carrot Plants to Citrus Plants by Trioza Erytreae

Insects ◽  
2020 ◽  
Vol 11 (8) ◽  
pp. 514 ◽  
Author(s):  
María Quintana-González de Chaves ◽  
Gabriela R. Teresani ◽  
Estrella Hernández-Suárez ◽  
Edson Bertolini ◽  
Aránzazu Moreno ◽  
...  
Keyword(s):  
Citrus Industry ◽  
Mainland Portugal ◽  
Trioza Erytreae ◽  
Candidatus Liberibacter ◽  
Citrus Huanglongbing ◽  
Efficient Vector ◽  
Inoculation Access Period ◽  
The Mediterranean ◽  
Low Efficiency ◽  
The Mediterranean Basin

Bacteria belonging to ‘Candidatus Liberibacter spp.’ are associated with various severe diseases in the five continents. The African citrus psyllid Trioza erytreae (Hemiptera: Triozidae) is an efficient vector of citrus huanglongbing-HLB disease, absent in the Mediterranean basin. This psyllid is currently present in the islands and mainland Portugal and Spain, where the prevalence of ‘Ca. Liberibacter solanacearum’ (CaLsol) associated to a carrot disease is high. Trioza erytreae normally feeds on citrus plants but has also been observed on other crops. It would be a great concern to the Mediterranean citrus industry if T. erytreae could transmit this bacterium from carrots to citrus and cause disease; therefore, the transmission of CaLsol from carrot plants to citrus plants was experimentally assessed. Although CaLsol was initially detected on receptor citrus plants in transmission assays by dodder and budding, the infection was not established. The feeding behavior by electrical penetration graphs and oviposition of T. erytreae on carrot plants versus citrus plants was evaluated. Trioza erytreae only reached the phloem in citrus plants. However, it was able to acquire CaLsol from infected carrots but unable to transmit it to citrus plants. CaLsol was detected in some carrot plants immediately after 7 and 14 days (inoculation access period), but it was not detected after one month. Trioza erytreae was unable to complete its life cycle on carrot plants. In conclusion, the efficient vector of bacteria associated to huanglongbing was unable to transmit CaLsol from carrot to citrus plants, but it acquired and transmitted the bacterium from carrot to carrot plants with low efficiency.

scholarly journals Does the African Citrus psyllid, Trioza erytreae (Del Guercio) (Hemiptera: Triozidae), Represent a Phytosanitary Threat to the Citrus Industry in Mexico?

Insects ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 450
Author(s):  
Saúl Espinosa-Zaragoza ◽  
Nidia Bélgica Pérez-De la O ◽  
Juan Francisco Aguirre-Medina ◽  
Víctor López-Martínez
Keyword(s):  
Ecological Niche Modeling ◽  
Vital Role ◽  
Point Of View ◽  
Trioza Erytreae ◽  
Entry Points ◽  
Wide Availability ◽  
Casimiroa Edulis ◽  
Citrus Production ◽  
Candidatus Liberibacter ◽  
Environmental Availability

The African citrus psyllid, Trioza erytreae (Del Guercio) (Hemiptera: Triozidae), is a vector of Candidatus Liberibacter africanus (CLaf), a pathogen that causes huanglongbing (HLB) in Africa. Trioza erytreae has invaded areas of Asia and Europe and has threatened citrus production due to its biological habits and the transmission of CLaf. Mexico is a country where citrus production has a vital role from the economic and social point of view. Therefore, ecological niche modeling (ENM) was used to determine if Mexico has the environmental availability that will allow T. erytreae invasion. We analyzed whether or not the distribution of Casimiroa edulis La Llave (Rutaceae) in the country could be a factor that enables the dispersal of T. eytreae. The environmental connectivity between five points of entry into the country (two ports and three airports) was explored to determine possible routes of dispersal of T. erytrae. The results showed that Mexico has wide availability for the invasion of the African citrus psyllid, which coincides with essential citrus areas of the country and with the distribution of C. edulis. Of the entry points studied, the Port of Veracruz showed nearby areas with environmental connectivity. Preventive monitoring measures for T. erytreae in Mexico should focus on Veracruz state because it has an entry point, ideal environmental availability, citrus areas, and specimens of C. edulis.

Thysanoptera diversity associated with Mexican lemon (Citrus aurantifolia Christm.)

2020 ◽  
Vol 13 (9) ◽  
Author(s):  
Francisco Javier Avendaño-Gutiérrez ◽  
Salvador Aguirre Paleo ◽  
Alejandro Morales Hernández ◽  
Venecia Quesadas-Béjar
Keyword(s):  
Biosphere Reserve ◽  
Simple Random Sampling ◽  
Diversity Indices ◽  
Diaphorina Citri ◽  
Program Calculation ◽  
Homogeneous Relation ◽  
Candidatus Liberibacter ◽  
Scirtothrips Citri ◽  
Species Richness And Abundance ◽  
First Time

Objective: To calculate the monthly relative abundance of Thysanoptera species, according to the Margalef, Simpson and Shannon-Wiener diversity indices. Design / Methodology / Approach: The work was carried out in three geographic units with conventional management, during January-December, 2019 in the Reserva de la Biosfera Zicuirán-Infiernillo (Biosphere Reserve), Michoacán, Mexico. In each unit, 10 trees were selected through simple random sampling. Thrips counts were performed on ten shoots per tree every 15 d, for a total of 7200 shoots in the three geographic units. Thysanoptera individuals were placed in entomological jars. The variables were: number of thrips collected per shoot in sampled tree and geographic unit (orchard). To estimate the specific richness and structure of species, the program "calculation of diversity indices DIVERS" was used. Results: In the three geographical units studied, the recorded presence of Thysanoptera accounted for 12 to 17 species. For Nueva Italia 12 recorded species, two were permanent (16.66%), five abundant (41.66%), one scarce (8.3%) and four rare (33.33%). In Zicuirán, three species were permanent (17.64%), six abundant (35.29%), two scarce (11.76%) and six rare (35.29%). In Los Hoyos, four species were permanent (26.66%), four abundant (26.66%) and seven rare (46.66%). The abundance of species was represented by the genus Frankliniella and the species Scolothrips sexmaculatus and Scirtothrips citri. The highest species richness and abundance was found from January to May. In October and November, the value of the calculated indices was zero, which shows less richness and abundance of individuals. The best species uniformity was recorded during January and December, which meant a more stable and homogeneous relation. Study limitations/Implications. Pest resurgence, presence of Candidatus Liberibacter spp. and its vector Diaphorina citri. Findings / Conclusions: in Nueva Italia, 12 species were taxonomically determined; in Los Hoyos 15, and in Zicuirán 17 species, which are reported for the first time in the state of Michoacán, Mexico. At the geographic unit "Los Hoyos" diversity was higher, uniform and stable.

Actividad de dos hongos entomopatógenos, identificados molecularmente, sobre Bactericera cockerelli

2017 ◽  
Vol 43 (1) ◽  
pp. 27 ◽  
Author(s):  
Fabiola Villegas-Rodríguez ◽  
Ovidio Díaz-Gómez ◽  
J. Sergio Casas-Flores ◽  
Clara Teresa Monreal-Vargas ◽  
Fernando Tamayo-Mejía ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Bactericera Cockerelli ◽  
Candidatus Liberibacter

El psílido de la papa Bactericera cockerelli afecta a las solanáceas en México, por el daño directo que causa, además, de que es transmisor de patógenos procariotes como Candidatus Liberibacter solanacerum (psyllaurous). Este insecto es combatido principalmente con productos químicos. Sin embargo, en la actualidad, se buscan nuevas estrategias para su manejo como el uso de hongos entomopatógenos. El objetivo de este trabajo fue evaluar la susceptibilidad del psílido de la papa a dos cepas comerciales y dos nativas de Metarizhium anisopliae y Beauveria bassiana, así como confirmar la identificación de las mismas mediante técnicas moleculares. Los bioensayos se realizaron con la cepa nativa MA25 de M. anisopliae y las comerciales Metabich® (MA28) y Bassianil® (BB09) y la nativa BB42 de Be. bassiana. Se probaron cinco concentraciones sobre ninfas del tercer estadio de B. cockerelli y se obtuvieron sus CL50 y CL95. Todas las cepas resultaron patógenas para el psílido de la papa, las más tóxicas fueron BB09, BB42 y MA28. La mortalidad fluctuó entre 90 a 100% con las concentraciones más altas de cada cepa: la más virulenta fue BB09, con una CL50 de 2,99 x 104 conidias mL-1 y MA25 la menos virulenta tuvo una CL50 de 6,34 x 105 conidias ml-1. Mediante la clonación y la secuenciación de la región ITS de los rADN 18S, se corroboró que las cepas nativas identificadas por morfología microscópica y claves taxonómicas corresponden a Be. bassiana y M. anisopliae.

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