scholarly journals Combined effects of systemic parathyroid hormone (1–34) and locally delivered neutral self‐assembling peptide hydrogel in the treatment of periodontal defects: An experimental in vivo investigation

2019 ◽  
Vol 46 (10) ◽  
pp. 1030-1040 ◽  
Author(s):  
Wataru Yoshida ◽  
Daisuke Matsugami ◽  
Tasuku Murakami ◽  
Takahiro Bizenjima ◽  
Kentaro Imamura ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Combined Effects ◽  
Self Assembling ◽  
Peptide Hydrogel

scholarly journals Investigating the In Vivo Antimicrobial Activity of a Self-Assembling Peptide Hydrogel Using a Galleria mellonella Infection Model

ACS Omega ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 2584-2589 ◽  
Author(s):  
Alice P. McCloskey ◽  
Merissa Lee ◽  
Julianne Megaw ◽  
Judith McEvoy ◽  
Sophie M. Coulter ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Galleria Mellonella ◽  
Infection Model ◽  
Self Assembling ◽  
Peptide Hydrogel

Targeted Chemotherapy for Breast Cancer Using an Intelligent Doxorubicin-Loaded Hexapeptide Hydrogel

2020 ◽  
Vol 16 (6) ◽  
pp. 842-852
Author(s):  
Shiyao Luo ◽  
Ying Zhu ◽  
Yiping Li ◽  
Li Chen ◽  
Shunzhong Lv ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Solid Phase ◽  
Synthesis Method ◽  
High Water ◽  
High Water Content ◽  
Normal Tissues ◽  
Self Assembling ◽  
Peptide Hydrogel

Self-assembling peptide hydrogels have a high water content, good biocompatibility and have become a competitive research object in the fields of tissue engineering, cancer treatment and drug delivery. In our research, a hexapeptide with high pH sensitivity was designed and synthesized by utilizing a solid-phase synthesis method. Under physiological conditions, the peptide could self-assemble into a hydrogel. When it reached the tumor acidic microenvironment, the peptide was degraded and doxorubicin was released to exert its antitumor effect. A series of physicochemical properties were investigated, including gelling ability, secondary structure, micromorphology, rheological properties and drug release studies. The results illustrated that PIDO peptide hydrogel has good pH responsiveness and injectability. In vitro cytotoxicity experiments and in vivo antitumor experiments showed that PIDO peptide hydrogel has a highly effective therapeutic effect on tumor cells and is less toxic to normal tissues. Our research provides a promising option for targeted drug delivery and sustainable release.

scholarly journals Feasibility of a self‐assembling peptide hydrogel scaffold for meniscal defect: An in vivo study in a rabbit model

2020 ◽  
Vol 39 (1) ◽  
pp. 165-176
Author(s):  
Nobuhiro Okuno ◽  
Shuhei Otsuki ◽  
Jo Aoyama ◽  
Kosuke Nakagawa ◽  
Tomohiko Murakami ◽  
...  
Keyword(s):  
Rabbit Model ◽  
In Vivo Study ◽  
Hydrogel Scaffold ◽  
Self Assembling ◽  
Peptide Hydrogel

scholarly journals Development and in vivo evaluation of intranasal formulations of parathyroid hormone (1-34)

Drug Delivery ◽  
2021 ◽  
Vol 28 (1) ◽  
pp. 487-498
Author(s):  
Dan Wang ◽  
Yimeng Du ◽  
Wenpeng Zhang ◽  
Xiaolu Han ◽  
Hui Zhang ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
In Vivo Evaluation

Exogenous parathyroid hormone attenuates ovariectomy-induced skeletal muscle weakness in vivo

Bone ◽  
2021 ◽  
pp. 116029
Author(s):  
Taro Fujimaki ◽  
Takashi Ando ◽  
Takanori Hata ◽  
Yoshihiro Takayama ◽  
Tetsuro Ohba ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Parathyroid Hormone ◽  
Muscle Weakness ◽  
Skeletal Muscle Weakness

scholarly journals Newly Developed Self-Assembling Antioxidants as Potential Therapeutics for the Cancers

2021 ◽  
Vol 11 (2) ◽  
pp. 92 ◽  
Author(s):  
Babita Shashni ◽  
Yukio Nagasaki
Keyword(s):  
Cancer Survival ◽  
Self Assembling ◽  
Therapeutic Regime ◽  
Cancer Models ◽  
Secondary Messengers ◽  
Potential Therapeutics ◽  
Target Effects ◽  
Tempo Radical

Elevated reactive oxygen species (ROS) have been implicated as significant for cancer survival by functioning as oncogene activators and secondary messengers. Hence, the attenuation of ROS-signaling pathways in cancer by antioxidants seems a suitable therapeutic regime for targeting cancers. Low molecular weight (LMW) antioxidants such as 2,2,6,6-tetramethylpyperidine-1-oxyl (TEMPO), although they are catalytically effective in vitro, exerts off-target effects in vivo due to their size, thus, limiting their clinical use. Here, we discuss the superior impacts of our TEMPO radical-conjugated self-assembling antioxidant nanoparticle (RNP) compared to the LMW counterpart in terms of pharmacokinetics, therapeutic effect, and adverse effects in various cancer models.

scholarly journals Extracellular vesicles-released parathyroid hormone-related protein from Lewis lung carcinoma induces lipolysis and adipose tissue browning in cancer cachexia

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wenjun Hu ◽  
Hairong Xiong ◽  
Zeyuan Ru ◽  
Yan Zhao ◽  
Yali Zhou ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Parathyroid Hormone ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Cancer Cachexia ◽  
Lewis Lung ◽  
Related Protein ◽  
Parathyroid Hormone Related Protein ◽  
Tissue Browning

AbstractCancer cachexia is a metabolic disorder characterized by skeletal muscle wasting and white adipose tissue browning. Specific functions of several hormones, growth factors, and cytokines derived from tumors can trigger cachexia. Moreover, adipose tissue lipolysis might explain weight loss that occurs owing to cachexia. Extracellular vesicles (EVs) are involved in intercellular communication. However, whether EVs participate in lipolysis induced by cancer cachexia has not been thoroughly investigated. Using Lewis lung carcinoma (LLC) cell culture, we tested whether LLC cell-derived EVs can induce lipolysis in 3T3-L1 adipocytes. EVs derived from LLC cells were isolated and characterized biochemically and biophysically. Western blotting and glycerol assay were used to study lipolysis. LLC cell-derived EVs induced lipolysis in vivo and vitro. EVs fused directly with target 3T3-L1 adipocytes and transferred parathyroid hormone-related protein (PTHrP), activating the PKA signaling pathway in 3T3-L1 adipocytes. Blocking PTHrP activity in LLC-EVs using a neutralizing antibody and by knocking down PTHR expression prevented lipolysis in adipocytes. Inhibiting the PKA signaling pathway also prevents the lipolytic effects of EVs. In vivo, suppression of LLC-EVs release by knocking down Rab27A alleviated white adipose tissue browning and lipolysis. Our data showed that LLC cell-derived EVs induced adipocyte lipolysis via the extracellular PTHrP-mediated PKA pathway. Our data demonstrate that LLC-EVs induce lipolysis in vitro and vivo by delivering PTHrP, which interacts with PTHR. The lipolytic effect of LLC-EVs was abrogated by PTHR knockdown and treatment with a neutralizing anti-PTHrP antibody. Together, these data show that LLC-EV-induced lipolysis is mediated by extracellular PTHrP. These findings suggest a novel mechanism of lipid droplet loss and identify a potential therapeutic strategy for cancer cachexia.

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