Non-bacterial protein expression in periodontal pockets by proteome analysis

2013 ◽  
Vol 40 (6) ◽  
pp. 573-582 ◽  
Author(s):  
Carlo Bertoldi ◽  
Elisa Bellei ◽  
Chiara Pellacani ◽  
Davide Ferrari ◽  
Andrea Lucchi ◽  
...  
Keyword(s):  
Protein Expression ◽  
Proteome Analysis ◽  
Bacterial Protein

2D-DIGE A Powerful Tool for Proteome Analysis

2020 ◽  
pp. 67-73
Keyword(s):  
Protein Expression ◽  
Gel Electrophoresis ◽  
Proteome Analysis ◽  
Great Sensitivity ◽  
Two Dimensional Gel Electrophoresis ◽  
2D Dige ◽  
Protein Sample ◽  
Different Types ◽  
Complex Protein ◽  
2D Gel

In the recent past, two dimensional gel electrophoresis has emerged as a powerful molecular biology tool for the comparative expression profiling of complex protein sample. It involves the separation as well as the resolution of diverse proteins sample on the basis of isoelectric points and molecular mass of protein in two dimension ways. In this way, it reflects the view of overall proteome status including differentiation in protein expression levels, post-translational modifications etc. Moreover, this allows the identification of novel biological signatures, which may give a particular identity of pathological background to cells or tissues associated with various types of cancers and neurological disorders. Therefore, by utilizing such tools, one can clearly investigate and compare the effects of particular drugs on cells of tissues and also one can analyze the effects of disease on the basis of variations in protein expression profile at broad spectrum. Recently, to get more error-less and accurate proteome profile, conventional 2-D gel electrophoresis has been enhanced with the inclusion of different types of protein labeling dyes which enables a more comparative analysis of diverse protein sample in a single 2-D gel. In this advanced technique (2-D-DIGE), protein samples are labeled with three different types of CyDyes (Cy2, Cy3, and Cy5) separately and combined and further resolved on the same gel. This will facilitate the more accurate spot matching on a single gel platform and will also minimize the experimental variations as commonly reported in the conventional 2D-gel electrophoresis. Therefore, in the present proteomic research era, 2D-DIGE has proved to be an extremely powerful tool with great sensitivity for up to 125 ng of proteins in clinical research volubility especially, neurological and cancer related disorders.

scholarly journals Rapid Proteome Analysis of Bronchoalveolar Lavage Samples of Lifelong Smokers and Never-Smokers by Micro-Scale Liquid Chromatography and Mass Spectrometry

2006 ◽  
Vol 52 (4) ◽  
pp. 671-679 ◽  
Author(s):  
Amelie Plymoth ◽  
Ziping Yang ◽  
Claes-Göran Löfdahl ◽  
Ann Ekberg-Jansson ◽  
Magnus Dahlbäck ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Protein Expression ◽  
Bronchoalveolar Lavage ◽  
Proteomic Analysis ◽  
Ion Trap ◽  
Wide Spectrum ◽  
Proteome Analysis ◽  
Chronic Obstructive ◽  
Never Smokers

Abstract Background: The aim of this study was to determine whether relative qualitative and quantitative differences in protein expression could be related to smoke exposure or smoke-induced airway inflammation. We therefore explored and characterized the protein components found in bronchoalveolar lavage (BAL) fluid sampled from either lifelong smokers or never-smokers. Methods: BAL fluid samples obtained by bronchoscopy from 60-year-old healthy never-smokers (n = 18) and asymptomatic smokers (n = 30) were analyzed in either pooled or individual form. Initial global proteomic analysis used shotgun digestion approaches on unfractionated BAL fluid samples (after minimal sample preparation) and separation of peptides by gradient (90-min) liquid chromatography (LC) coupled with on-line linear ion trap quadropole mass spectrometry (LTQ MS) for identification and analysis. Results: LTQ MS identified 481 high- to low-abundance proteins. Relative differences in patterns of BAL fluid proteins in smokers compared with never-smokers were observed in pooled and individual samples as well as by 2-dimensional gel analysis. Gene ontology categorization of all annotated proteins showed a wide spectrum of molecular functions and biological processes. Conclusions: The described method provides comprehensive qualitative proteomic analysis of BAL fluid protein expression from never-smokers and from smokers at risk of developing chronic obstructive pulmonary disease. Many of the proteins identified had not been detected in previous studies of BAL fluid; thus, the use of LC-tandem MS with LTQ may provide new information regarding potentially important patterns of protein expression associated with lifelong smoking.

Proteome analysis of isolated myenteric plexus reveals significant changes in protein expression during postnatal development

2005 ◽  
Vol 122 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Cornelia Irene Hagl ◽  
Olivia Thil ◽  
Stefan Holland-Cunz ◽  
Ralf Faissner ◽  
Silke Wandschneider ◽  
...  
Keyword(s):  
Protein Expression ◽  
Postnatal Development ◽  
Myenteric Plexus ◽  
Proteome Analysis

scholarly journals Integrated transcriptome and proteome analysis reveals brassinosteroid-mediated regulation of cambium initiation and patterning in woody stem.

2022 ◽  
Vol 9 ◽  
Author(s):  
Congpeng Wang ◽  
Naixu Liu ◽  
Zhao Geng ◽  
Meijing Ji ◽  
Shumin Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Proteome Analysis ◽  
Wood Formation ◽  
Integrated Analysis ◽  
Xylem Differentiation ◽  
Transgenic Poplar ◽  
Wood Development ◽  
Procambial Cell ◽  
Proteome Level ◽  
Mutant Lines

Abstract Wood formation involves sequential developmental events requiring the coordination of multiple hormones. Brassinosteroids (BRs) play a key role in wood development, but little is known about the cellular and molecular processes that underlie wood formation in tree species. Here, we generated transgenic poplar lines with edited PdBRI1 genes, which are orthologs of Arabidopsis vascular-enriched BR receptors, and showed how inhibition of BR signaling influences wood development at the mRNA and/or proteome level. Six Populus PdBRI1 genes formed three gene pairs, each of which was highly expressed in basal stems. Simultaneous mutation of PdBRI1–1, −2, −3 and − 6, which are orthologs of the Arabidopsis vascular-enriched BR receptors BRI1, BRL1 and BRL3, resulted in severe growth defects. In particular, the stems of these mutant lines displayed a discontinuous cambial ring and patterning defects in derived secondary vascular tissues. Abnormal cambial formation within the cortical parenchyma was also observed in the stems of pdbri1–1;2;3;6. Transgenic poplar plants expressing edited versions of PdBRI1–1 or PdBRI1–1;2;6 exhibited phenotypic alterations in stem development at 4.5 months of growth, indicating that there is functional redundancy among these PdBRI1 genes. Integrated analysis of the transcriptome and proteome of pdbri1–1;2;3;6 stems revealed differential expression of a number of genes/proteins associated with wood development and hormones. Concordant (16%) and discordant (84%) regulation of mRNA and protein expression, including wood-associated mRNA/protein expression, was found in pdbri1–1;2;3;6 stems. This study found a dual role of BRs in procambial cell division and xylem differentiation and provides insights into the multiple layers of gene regulation that contribute to wood formation in Populus.

White muscle proteome analysis showing insights into the protein expression in orange-spotted grouper (Epinephelus coioides) muscle

2020 ◽  
Vol 36 ◽  
pp. 100655
Author(s):  
Yuan-Tih Ko ◽  
Shih-Chin Hsu ◽  
Bartosz Kazłowski ◽  
Fu-Hsiung Huang ◽  
Chyng-Hwa Liou
Keyword(s):  
Protein Expression ◽  
White Muscle ◽  
Proteome Analysis ◽  
Epinephelus Coioides ◽  
Muscle Proteome

Optoregulated Protein Release from an Engineered Living Material

2018 ◽  
Author(s):  
Shrikrishnan Sankaran ◽  
Aránzazu del Campo
Keyword(s):  
Protein Expression ◽  
Protein Secretion ◽  
Fluorescent Protein ◽  
Protein Release ◽  
Light Irradiation ◽  
Bacterial Protein ◽  
Immobilized Bacteria ◽  
Living Material

An engineered living material for light-regulated protein release is realized by combining optogenetics, bacterial protein secretion and hydrogels. Hydrogel-immobilized bacteria are optogenetically programmed to express and secrete a fluorescent protein in response to light irradiation. Using light, tunability and localized confinement of protein expression along with dosing of protein release are demonstrated.

Study on the Mechanisms of CML Blast Crisis by Comparative Proteome Analysis.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4770-4770
Author(s):  
Xiaoli Liu ◽  
Qingfeng Du ◽  
Song Zhang ◽  
Rong Li ◽  
Feng Yao ◽  
...  
Keyword(s):  
Protein Expression ◽  
Blast Crisis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Chronic Phase ◽  
Proteome Analysis ◽  
Terminal Phase ◽  
Proteasome Subunit ◽  
Peptide Mass ◽  
Significant Difference ◽  
Leukotriene A4

Abstract The clinical course of chronic myeloid leukemia(CML)is characteristically triphasic, comprising chronic and accelerated phases and blast crisis. Chronic phase(CP) is characterized by the Ph chromosome as the sole genetic abnormality and blast crisis(BC), which is the terminal phase of CML, often associated with additional chromosomal and molecular secondary changes. Although CML is probably the most extensively studied human malignancy, the mechanisms of CML blast crisis are still poorly understood. In current study, we analyzed the changes of protein expression between CML-CP(25 cases) and CML-BC(20 cases) by Two-dimensional polyacrylamide gel electrophoresis(2-D PAGE). Compared with that of CML-CP, 33 proteins’ intensities of CML-BC were found to have significant difference including 23 increasing and 13 decreasing. 15 proteins were identified by peptide mass fingerprint in combination with database searching including proteasome activator complex, hnRNP, annexin A4, serine proteinase inhibitor, annexin A1, GAPDH, RhoGDI, enolase, proteasome subunit 6a, GTP binding protein, leukotriene A4 hydrolase, Rac-RhoGDI, thioredoxin, proteasome subunit 4β and DJ-1 protein, and the functions of these proteins involve cell signal transduction, apoptosis, proliferation and transcription. In conclusion, our study provided a profile of protein expression difference between CML-CP and CML-BC and contributed to understand the mechanisms of CML blast crisis.

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