scholarly journals 3D culture of cancer cells in alginate hydrogel beads as an effective technique for emergency cell storage and transportation in the pandemic era

2021 ◽  
Author(s):  
Batoul Alallam ◽  
May Kyaw Oo ◽  
Wisam Nabeel Ibrahim ◽  
Abd Almonem Doolaanea
Keyword(s):  
Cancer Cells ◽  
3D Culture ◽  
Alginate Hydrogel ◽  
Effective Technique ◽  
Hydrogel Beads ◽  
Cell Storage

scholarly journals Recombinant Human erythropoietin reduces viability of MCF-7 breast cancer cells from 3D culture without caspase activation

2021 ◽  
Vol 28 (4) ◽  
pp. 2549-2557
Author(s):  
Hareth Y. ShujaaEdin ◽  
Nagi A. AL-Haj ◽  
Abdullah Rasedee ◽  
Noorjahan Banu Alitheen ◽  
Arifah Abdul Kadir ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Recombinant Human Erythropoietin ◽  
Breast Cancer Cells ◽  
3D Culture ◽  
Caspase Activation ◽  
Human Erythropoietin ◽  
Mcf 7

Construction of multilayer alginate hydrogel beads for oral delivery of probiotics cells

2017 ◽  
Vol 105 ◽  
pp. 924-930 ◽  
Author(s):  
Yang Li ◽  
Chao Feng ◽  
Jing Li ◽  
Yuzhi Mu ◽  
Ya Liu ◽  
...  
Keyword(s):  
Oral Delivery ◽  
Alginate Hydrogel ◽  
Hydrogel Beads

scholarly journals Epigenetic Therapy Augments Classic Chemotherapy in Suppressing the Growth of 3D High-Grade Serous Ovarian Cancer Spheroids over an Extended Period of Time

Biomolecules ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1711
Author(s):  
Michelle Bilbao ◽  
Chelsea Katz ◽  
Stephanie L. Kass ◽  
Devon Smith ◽  
Krystal Hunter ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
3D Culture ◽  
Extended Period ◽  
Recurrent Ovarian Cancer ◽  
Epigenetic Therapy ◽  
Ovarian Cancer Cells ◽  
High Grade ◽  
Serous Ovarian Cancer ◽  
Cancer Spheroids

Recurrent high-grade serous ovarian cancer (HGSC) is clinically very challenging and prematurely shortens patients’ lives. Recurrent ovarian cancer is characterized by high tumor heterogeneity; therefore, it is susceptible to epigenetic therapy in classic 2D tissue culture and rodent models. Unfortunately, this success has not translated well into clinical trials. Utilizing a 3D spheroid model over a period of weeks, we were able to compare the efficacy of classic chemotherapy and epigenetic therapy on recurrent ovarian cancer cells. Unexpectedly, in our model, a single dose of paclitaxel alone caused the exponential growth of recurrent high-grade serous epithelial ovarian cancer over a period of weeks. In contrast, this effect is not only opposite under treatment with panobinostat, but panobinostat reverses the repopulation of cancer cells following paclitaxel treatment. In our model, we also demonstrate differences in the drug-treatment sensitivity of classic chemotherapy and epigenetic therapy. Moreover, 3D-derived ovarian cancer cells demonstrate induced proliferation, migration, invasion, cancer colony formation and chemoresistance properties after just a single exposure to classic chemotherapy. To the best of our knowledge, this is the first evidence demonstrating a critical contrast between short and prolonged post-treatment outcomes following classic chemotherapy and epigenetic therapy in recurrent high-grade serous ovarian cancer in 3D culture.

Fabrication of an efficient ZIF-8 alginate composite hydrogel material and its application for enhanced copper (II) adsorption from aqueous solutions

2021 ◽  
Author(s):  
Xiao Zhang ◽  
Zhiyue Li ◽  
Taoyi Zhang ◽  
Jing Chen ◽  
Wenxi Ji ◽  
...  
Keyword(s):  
Aqueous Solutions ◽  
Sodium Alginate ◽  
Composite Hydrogel ◽  
Alginate Hydrogel ◽  
Zeolitic Imidazolate Framework ◽  
Composite Bead ◽  
Hydrogel Beads

A novel zeolitic imidazolate framework-8 (ZIF-8) alginate composite hydrogel material (PVA/SA@ZIF-8) is alginate composite bead which was fabricated by grafting ZIF-8 on the surface of the sodium alginate hydrogel beads...

Alginate hydrogel – candidate support for cell transplantation

e-Polymers ◽  
2005 ◽  
Vol 5 (1) ◽  
Author(s):  
Piotr Woźniak ◽  
Marek Kozicki ◽  
Janusz M. Rosiak ◽  
Jacek Przybylski ◽  
Malgorzata Lewandowska-Szumieł
Keyword(s):  
Cell Transplantation ◽  
Quantitative Study ◽  
Alginate Hydrogel ◽  
Human Cartilage ◽  
Factors Affecting ◽  
Hydrogel Beads ◽  
Encapsulated Cells ◽  
Candidate Support

AbstractHuman cartilage derived cells were encapsulated in alginate hydrogel beads. Viability of the cells was determined using both qualitative (microscopy) and quantitative (MTT and NR assays) examination. Microscopy indicated that encapsulated cells remained viable during the 7-days experiment. The quantitative tests failed to give reliable results. Possible factors affecting the results of the quantitative study are discussed.

scholarly journals Epithelial–mesenchymal transition and metastasis of colon cancer cells induced by the FAK pathway in cancer-associated fibroblasts

2020 ◽  
Vol 48 (6) ◽  
pp. 030006052093124
Author(s):  
Xuefeng Xuefeng ◽  
Ming-Xing Hou ◽  
Zhi-Wen Yang ◽  
Agudamu Agudamu ◽  
Feng Wang ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Epithelial Mesenchymal Transition ◽  
3D Culture ◽  
Cancer Associated Fibroblasts ◽  
Colon Cancer Cells ◽  
Mesenchymal Transition ◽  
Lovo Cells ◽  
Related Proteins

Objective The role and mechanism of tetrathiomolybdate (TM) in cancer-associated fibroblasts (CAFs) in colon cancer using three-dimensional (3D) culture were investigated, and the associations between the focal adhesion kinase (FAK) pathway and epithelial–mesenchymal transition (EMT) in CAFs were explored. Methods A 3D co-culture model of colon cancer LOVO cells with CAFs and normal fibroblasts (NFs) was established using Matrigel as a scaffold material. The differential expression of LOXL2 (lysyl oxidase-like 2) in the supernatant of CAFs and NFs was determined using ELISA, and expression levels of EMT-related proteins and FAK signaling pathway-related proteins were determined using western blot. Results LOXL2 levels secreted by CAFs were higher compared with that secreted by NFs. In the CAF + LOVO group, compared with the LOVO group, E-cadherin expression decreased significantly, while N-cadherin and F-PAK expression increased significantly. TM results were opposite compared with the above results. Conclusions CAFs stimulate EMT in human colon cancer LOVO cells by secreting LOXL2 to activate the FAK signaling pathway, thereby promoting tumor metastasis. TM inhibited the occurrence of EMT in the CAF-induced colon cancer LOVO cell line, thereby reducing the invasion and metastasis of colon cancer cells.

scholarly journals EXPRESSION OF THE MICROFOLD CELLS IN THREE-DIMENSIONAL COCULTURE SYSTEM FOR IN VITRO CULTIVATION OF HUMAN NOROVIRUS

2019 ◽  
pp. 71-74
Author(s):  
MIZANURFAKHRI GHAZALI ◽  
SHARANIZA AB-RAHIM ◽  
MUDIANA MUHAMAD
Keyword(s):  
Three Dimensional ◽  
In Vitro Cultivation ◽  
Optimum Number ◽  
M Cells ◽  
Apical Surface ◽  
Alginate Hydrogel ◽  
Human Norovirus ◽  
Protein Marker ◽  
Hydrogel Beads

Introduction: Human Norovirus (HuNoV), a food-borne virus is the leading cause for acute gastroenteritis. However, its inability to propagate in vitropersists as major challenge in understanding HuNoV biology.Objective: This study aims to determine an effective culture system for HuNoV.Methods: The Caco-2 cells were cocultured with Raji B cells on alginate hydrogel beads. Scanning electron microscopy (SEM) was performed to confirmthe three-dimensional (3D) cells morphology. Western blot (WB) analysis was performed to detect protein markers expressed by Microfold (M) cells.Results: Optimization of Caco-2 cells monoculture in the alginate hydrogel beads showed optimum number of cells of 1 × 106 cells/ml, indicatedby the intact structure of the beads. Result of SEM showed clear structure of monoculture in the alginate hydrogel beads indicated by the presenceof smooth and regular apical surface while the coculture showed reduced apical surface of M cells. The result of WB showed downregulation ofUlex europaeus antibody expression.Conclusion: It is evident that the expression of M cells grown in 3D alginate hydrogel beads was successful, indicated by the structural morphologyseen under SEM as well as expression of protein marker by M cells. This established in vitro system is highly potential for cultivation of HuNoV.

scholarly journals The Leader Position of Mesenchymal Cells Expressing N-Cadherin in the Collective Migration of Epithelial Cancer

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 731 ◽  
Author(s):  
Inés Saénz-de-Santa-María ◽  
Lucía Celada ◽  
María-Dolores Chiara
Keyword(s):  
Head And Neck ◽  
Cancer Cells ◽  
Disease Progression ◽  
3D Culture ◽  
Mesenchymal Cells ◽  
Collective Migration ◽  
Epithelial Cancer ◽  
Human Carcinoma ◽  
Squamous Carcinoma Cells ◽  
The Impact

Understanding how heterogeneous cancer cell populations migrate collectively is of paramount importance to arrest metastasis. Here, we applied 3D culture-based approaches for in vitro modeling of the collective migration of squamous carcinoma cells and examine the impact of epithelial and mesenchymal cell interactions on this type of migration. We show that both mesenchymal N-cadherin-expressing cancer cells and cancer-associated fibroblasts cooperate in collective migration of epithelial cancer cells by leading their collective migration. This was consistent with the observed distribution of E-cadherin/N-cadherin in the human carcinoma tissues of head and neck. The presence of “leader” mesenchymal cancer cells or “leader” fibroblasts was significantly associated with metastasis development, recurrent disease and low overall disease survival in head and neck squamous cell carcinomas (HNSCC). In silico analysis of independent public datasets revealed that increased N-cadherin expression in the heterogeneous cancer tissues is associated with disease progression not only in HNSCC but also in other prevalent tumors, such as colorectal, breast and lung cancer. Collectively, our data highlight the importance of mesenchymal cells in collective cell migration and disease progression, findings that may have a broad significance in cancer, especially in those in which aberrant N-cadherin expression negatively impacts disease survival.

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