scholarly journals CircTLK1 modulates sepsis‐induced cardiomyocyte apoptosis via enhancing PARP1/HMGB1 axis–mediated mitochondrial DNA damage by sponging miR‐17‐5p

2021 ◽  
Vol 25 (17) ◽  
pp. 8244-8260
Author(s):  
Yu Qiu ◽  
Ying Yu ◽  
Xiao‐Mei Qin ◽  
Tao Jiang ◽  
Yan‐Fang Tan ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Cardiomyocyte Apoptosis ◽  
Mitochondrial Dna Damage

OGG1 Genetic Polymorphism Is Associated with Mitochondrial DNA Damage in Pesticide-Exposed Fruit Growers

Epidemiology ◽  
2009 ◽  
Vol 20 ◽  
pp. S99
Author(s):  
Ruey-Hong Wong ◽  
Pei-Lin Huang ◽  
Yu-Hsuan Yeh ◽  
Yu-Ching Chen ◽  
Yi-Jie Liu ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Genetic Polymorphism ◽  
Mitochondrial Dna Damage

scholarly journals Mitochondrial DNA damage is associated with damage accrual and disease duration in patients with Systemic Lupus Erythematosus

Lupus ◽  
2014 ◽  
Vol 23 (11) ◽  
pp. 1133-1141 ◽  
Author(s):  
L López-López ◽  
M Nieves-Plaza ◽  
M del R Castro ◽  
YM Font ◽  
CA Torres-Ramos ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Dna Damage ◽  
Mitochondrial Dna ◽  
Lupus Erythematosus ◽  
Disease Duration ◽  
Systemic Lupus ◽  
Mitochondrial Dna Damage ◽  
Damage Accrual

A rapid assay for mitochondrial DNA damage and respiratory chain inhibition in the yeastSaccharomyces cerevisiae

2001 ◽  
Vol 38 (2-3) ◽  
pp. 153-158 ◽  
Author(s):  
Barry J. Barclay ◽  
Carrie L. DeHaan ◽  
Ursula G.G. Hennig ◽  
Oksana Iavorovska ◽  
Reid W. von Borstel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Respiratory Chain ◽  
Rapid Assay ◽  
Mitochondrial Dna Damage

Abstract 11852: Role of Lox-1 in Mitochondrial Dna Damage and NLRP3 Inflammasome Activation

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Zufeng Ding ◽  
Sadip Pant ◽  
Abhishek Deshmukh ◽  
Jawahar L Mehta
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Nlrp3 Inflammasome ◽  
Ros Generation ◽  
Inflammasome Activation ◽  
Mtdna Damage ◽  
Mitochondrial Dna Damage ◽  
Mitochondrial Ros ◽  
Nlrp3 Inflammasome Activation

Objective: This study tested the hypothesis that mitochondrial DNA damage could trigger NLRP3 inflammasome activation during inflammation, and LOX-1 may play a critical role in this process. Methods and Results: We performed studies in cultured human THP1 macrophages exposed to ox-LDL or LPS,which are often used as inflammation stimuli in vitro . We examined and confirmed the increase in LOX-1 expression when cells were treated with ox-LDL or LPS. Parallel groups of cells were treated with LOX-1 Ab to bind LOX-1. In accordance with our previous studies in endothelial cells and smooth muscle cells, LOX-1 Ab markedly reduced ox-LDL- as well as LPS-stimulated LOX-1 expression. To assess mitochondrial ROS generation, MitoSOX™ Red mitochondrial superoxide indicator was used. Both fluorescence staining and flow cytometry analysis showed that LPS induced (more than ox-LDL) mitochondrial ROS generation. Pretreatment with LOX-1 Ab significantly attenuated mitochondrial ROS generation in response to ox-LDL or LPS. Then we observed mtDNA damage in THP1 cells exposed to ox-LDL or LPS. Importantly, pretreatment with LOX-1 Ab protected mtDNA from damage in response to both stimuli. This was also confirmed by q-PCR (mtDNA/nDNA ratio) analysis. Further, ox-LDL or LPS induced the expression of phos-NF-kB p65, caspase-1 p10 and p20, and cleaved proteins IL-1β and IL-18. Of note, NLRP3 inflammasome was activated in response to ox-LDL or LPS in a similar manner. Pretreatment of cells with LOX-1 Ab treatment blocked or significantly attenuated these inflammatory responses. Conclusions: These observations based on in vitro observations indicate that LOX-1 via ROS generation plays a key role in mtDNA damage which then leads to NLRP3 inflammasome activation during inflammation.

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