A rapid assay for mitochondrial DNA damage and respiratory chain inhibition in the yeastSaccharomyces cerevisiae

2001 ◽  
Vol 38 (2-3) ◽  
pp. 153-158 ◽  
Author(s):  
Barry J. Barclay ◽  
Carrie L. DeHaan ◽  
Ursula G.G. Hennig ◽  
Oksana Iavorovska ◽  
Reid W. von Borstel ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Respiratory Chain ◽  
Rapid Assay ◽  
Mitochondrial Dna Damage

OGG1 Genetic Polymorphism Is Associated with Mitochondrial DNA Damage in Pesticide-Exposed Fruit Growers

Epidemiology ◽  
2009 ◽  
Vol 20 ◽  
pp. S99
Author(s):  
Ruey-Hong Wong ◽  
Pei-Lin Huang ◽  
Yu-Hsuan Yeh ◽  
Yu-Ching Chen ◽  
Yi-Jie Liu ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Genetic Polymorphism ◽  
Mitochondrial Dna Damage

scholarly journals Mitochondrial DNA damage is associated with damage accrual and disease duration in patients with Systemic Lupus Erythematosus

Lupus ◽  
2014 ◽  
Vol 23 (11) ◽  
pp. 1133-1141 ◽  
Author(s):  
L López-López ◽  
M Nieves-Plaza ◽  
M del R Castro ◽  
YM Font ◽  
CA Torres-Ramos ◽  
...  
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Dna Damage ◽  
Mitochondrial Dna ◽  
Lupus Erythematosus ◽  
Disease Duration ◽  
Systemic Lupus ◽  
Mitochondrial Dna Damage ◽  
Damage Accrual

Abstract 11852: Role of Lox-1 in Mitochondrial Dna Damage and NLRP3 Inflammasome Activation

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Zufeng Ding ◽  
Sadip Pant ◽  
Abhishek Deshmukh ◽  
Jawahar L Mehta
Keyword(s):  
Dna Damage ◽  
Mitochondrial Dna ◽  
Nlrp3 Inflammasome ◽  
Ros Generation ◽  
Inflammasome Activation ◽  
Mtdna Damage ◽  
Mitochondrial Dna Damage ◽  
Mitochondrial Ros ◽  
Nlrp3 Inflammasome Activation

Objective: This study tested the hypothesis that mitochondrial DNA damage could trigger NLRP3 inflammasome activation during inflammation, and LOX-1 may play a critical role in this process. Methods and Results: We performed studies in cultured human THP1 macrophages exposed to ox-LDL or LPS,which are often used as inflammation stimuli in vitro . We examined and confirmed the increase in LOX-1 expression when cells were treated with ox-LDL or LPS. Parallel groups of cells were treated with LOX-1 Ab to bind LOX-1. In accordance with our previous studies in endothelial cells and smooth muscle cells, LOX-1 Ab markedly reduced ox-LDL- as well as LPS-stimulated LOX-1 expression. To assess mitochondrial ROS generation, MitoSOX™ Red mitochondrial superoxide indicator was used. Both fluorescence staining and flow cytometry analysis showed that LPS induced (more than ox-LDL) mitochondrial ROS generation. Pretreatment with LOX-1 Ab significantly attenuated mitochondrial ROS generation in response to ox-LDL or LPS. Then we observed mtDNA damage in THP1 cells exposed to ox-LDL or LPS. Importantly, pretreatment with LOX-1 Ab protected mtDNA from damage in response to both stimuli. This was also confirmed by q-PCR (mtDNA/nDNA ratio) analysis. Further, ox-LDL or LPS induced the expression of phos-NF-kB p65, caspase-1 p10 and p20, and cleaved proteins IL-1β and IL-18. Of note, NLRP3 inflammasome was activated in response to ox-LDL or LPS in a similar manner. Pretreatment of cells with LOX-1 Ab treatment blocked or significantly attenuated these inflammatory responses. Conclusions: These observations based on in vitro observations indicate that LOX-1 via ROS generation plays a key role in mtDNA damage which then leads to NLRP3 inflammasome activation during inflammation.

Mitochondrial DNA damage in calf skeletal muscle and walking performance in people with peripheral artery disease

2020 ◽  
Vol 160 ◽  
pp. 680-689 ◽  
Author(s):  
Sunil K. Saini ◽  
Mary M. McDermott ◽  
Anna Picca ◽  
Lingyu Li ◽  
Stephanie E. Wohlgemuth ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Dna Damage ◽  
Mitochondrial Dna ◽  
Peripheral Artery Disease ◽  
Peripheral Artery ◽  
Mitochondrial Dna Damage ◽  
Walking Performance ◽  
Artery Disease
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