scholarly journals mTORC2 regulates hierarchical micro/nano topography‐induced osteogenic differentiation via promoting cell adhesion and cytoskeletal polymerization

2021 ◽  
Author(s):  
Qian Gao ◽  
Yuying Hou ◽  
Zhe Li ◽  
Jinyang Hu ◽  
Dawei Huo ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Osteogenic Differentiation

scholarly journals Testing the in vitro performance of hydroxyapatite coated magnesium (AZ91D) and titanium concerning cell adhesion and osteogenic differentiation

2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Claudia Kleinhans ◽  
Gabriele Vacun ◽  
Roman Surmenev ◽  
Maria Surmeneva ◽  
Petra Juliane Kluger
Keyword(s):  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Cell Attachment ◽  
Human Mesenchymal Stem Cell ◽  
Adhesion Properties ◽  
Sputtering Deposition ◽  
Initial Cell ◽  
Tissue Culture Polystyrene ◽  
Diffraction Patterns

AbstractIn the current study the in vitro outcome of a degradable magnesium alloy (AZ91D) and standard titanium modified by nanostructured-hydroxyapatite (n-HA) coatings concerning cell adhesion and osteogenic differentiation was investigated by direct cell culture. The n-HA modification was prepared via radio-frequency magnetron sputtering deposition and proven by field emission scanning electron microscopy and X-ray powder diffraction patterns revealing a homogenous surface coating. Human mesenchymal stem cell (hMSCs) adhesion was examined after one and 14 days displaying an enhanced initial cell adhesion on the n-HA modified samples. The osteogenic lineage commitment of the cells was determined by alkaline phosphatase (ALP) quantification. On day one n-HA coated AZ91D exhibited a comparable ALP expression to standard tissue culture polystyrene samples. However, after 14 days solely little DNA and ALP amounts were measurable on n-HA coated AZ91D due to the lack of adherent cells. Titanium displayed excellent cell adhesion properties and ALP was detectable after 14 days. An increased pH of the culture was measured for AZ91D as well as for n-HA coated AZ91D. We conclude that n-HA modification improves initial cell attachment on AZ91D within the first 24 h. However, the effect does not persist for 14 days in in vitro conditions.

KR-12 coating of polyetheretherketone (PEEK) surface via polydopamine improves osteointegration and antibacterial activity in vivo

2020 ◽  
Vol 8 (44) ◽  
pp. 10190-10204
Author(s):  
Xiangchao Meng ◽  
Jue Zhang ◽  
Jun Chen ◽  
Binen Nie ◽  
Bing Yue ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Material Surface

KR-12 was grafted onto the surface of PEEK, which improved the hydrophilicity of the material surface, promoted cell adhesion, osteogenic differentiation and osseointegration, while also endowing the appreciable antibacterial activity on the material surface.

Zwitterionic PMCP‐Modified Polycaprolactone Surface for Tissue Engineering: Antifouling, Cell Adhesion Promotion, and Osteogenic Differentiation Properties

Small ◽  
2019 ◽  
Vol 15 (42) ◽  
pp. 1903784 ◽  
Author(s):  
Xingyu Chen ◽  
Zaifu Lin ◽  
Ying Feng ◽  
Hong Tan ◽  
Xinyuan Xu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Adhesion Promotion

Cell adhesion and osteogenic differentiation on three-dimensional pillar surfaces

2012 ◽  
Vol 101A (3) ◽  
pp. 842-852 ◽  
Author(s):  
Emilia Kaivosoja ◽  
Pia Suvanto ◽  
Gonçalo Barreto ◽  
Susanna Aura ◽  
Antti Soininen ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Three Dimensional

scholarly journals Erratum to “Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression”

2018 ◽  
Vol 2018 ◽  
pp. 1-1
Author(s):  
Francesca Posa ◽  
Adriana Di Benedetto ◽  
Elisabetta A. Cavalcanti-Adam ◽  
Graziana Colaianni ◽  
Chiara Porro ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cell Adhesion ◽  
Osteogenic Differentiation

scholarly journals Vitamin D Promotes MSC Osteogenic Differentiation Stimulating Cell Adhesion and αVβ3 Expression

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Francesca Posa ◽  
Adriana Di Benedetto ◽  
Elisabetta A. Cavalcanti-Adam ◽  
Graziana Colaianni ◽  
Chiara Porro ◽  
...  
Keyword(s):  
Vitamin D ◽  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Matrix Protein ◽  
Active Form ◽  
Focal Adhesions ◽  
Supporting Cell ◽  
Integrin Expression ◽  
Dihydroxyvitamin D ◽  
Cell Commitment

Vitamin D (Vit D) by means of its biological active form, 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3), has a protective effect on the skeleton by acting on calcium homeostasis and bone formation. Furthermore, Vit D has a direct effect on mesenchymal stem cells (MSCs) in stimulating their osteogenic differentiation. In this work, we present for the first time the effect of 1,25(OH)2D3 on MSC adhesion. Considering that cell adhesion to the substrate is fundamental for cell commitment and differentiation, we focused on the expression of αVβ3 integrin, which has a key role in the commitment of MSCs to the osteoblastic lineage. Our data indicate that Vit D increases αVβ3 integrin expression inducing the formation of focal adhesions (FAs). Moreover, we assayed MSC commitment in the presence of the extracellular matrix (ECM) glycoprotein fibronectin (FN), which is able to favor cell adhesion on surfaces and also to induce osteopontin (OPN) expression: this suggests that Vit D and FN synergize in supporting cell adhesion. Taken together, our findings provide evidence that Vit D can promote osteogenic differentiation of MSCs through the modulation of αVβ3 integrin expression and its subcellular organization, thus favoring binding with the matrix protein (FN).

Amide-linkage formed between ammonia plasma treated poly(D,L-lactide acid) scaffolds and bio-peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Biopolymers ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 682-694 ◽  
Author(s):  
Zi-Xing Xu ◽  
Tao Li ◽  
Zhao-Ming Zhong ◽  
Ding-Sheng Zha ◽  
Song-Hui Wu ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Osteogenic Differentiation ◽  
Amide Linkage ◽  
Ammonia Plasma
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