scholarly journals The outer membrane protein Tp92 ofTreponema palliduminduces human mononuclear cell death and IL-8 secretion

2018 ◽  
Vol 22 (12) ◽  
pp. 6039-6054 ◽  
Author(s):  
Xi Luo ◽  
Xiaohong Zhang ◽  
Lin Gan ◽  
Chenglong Zhou ◽  
Tie Zhao ◽  
...  
Keyword(s):  
Cell Death ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Mononuclear Cell ◽  
Outer Membrane Protein ◽  
Human Mononuclear Cell

scholarly journals Outer Membrane Protein of Gut Commensal Microorganism Induces Autoantibody Production and Extra-Intestinal Gland Inflammation in Mice

2018 ◽  
Vol 19 (10) ◽  
pp. 3241 ◽  
Author(s):  
Naoko Yanagisawa ◽  
Hidehiro Ueshiba ◽  
Yoshihiro Abe ◽  
Hidehito Kato ◽  
Tomoaki Higuchi ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Salivary Glands ◽  
Outer Membrane ◽  
Mononuclear Cell ◽  
Outer Membrane Protein ◽  
Knockout Mice ◽  
The Body ◽  
Repeated Injection ◽  
E Coli ◽  
High Amino Acid

Gut commensal microorganisms have been linked with chronic inflammation at the extra-intestinal niche of the body. The object of the study was to investigate on the chronic effects of a gut commensal Escherichia coli on extra-intestinal glands. The presence of autoimmune response was diagnosed by autoantibody levels and histological methods. Repeated injection of E. coli induced mononuclear cell inflammation in the Harderian and submandibular salivary glands of female C57BL/6 mice. Inflammation was reproduced by adoptive transfer of splenocytes to immune-deficient Rag2 knockout mice and CD4+ T cells to mature T cell-deficient TCRβ-TCRδ knockout mice. MALDI TOF mass spectrometry of the protein to which sera of E. coli-treated mice reacted was determined as the outer membrane protein A (OmpA) of E. coli. Multiple genera of the Enterobacteriaceae possessed OmpA with high amino-acid sequence similarities. Repeated injection of recombinant OmpA reproduced mononuclear cell inflammation of the Harderian and salivary glands in mice and elevation of autoantibodies against Sjögren’s-syndrome-related antigens SSA/Ro and SSB/La. The results indicated the possibility of chronic stimuli from commensal bacteria-originated components as a pathogenic factor to elicit extra-intestinal autoimmunity.

Overexpression of Outer Membrane Protein A Is a Risk Factor for Mortality in Escherichia coli Bloodstream Infections

2019 ◽  
Author(s):  
Ángel Rodríguez-Villodres ◽  
Rocío Álvarez-Marín ◽  
María Antonia Pérez-Moreno ◽  
Andrea Miró-Canturri ◽  
Marco Durán Lobato ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Risk Factor ◽  
Membrane Protein ◽  
Outer Membrane ◽  
Outer Membrane Protein ◽  
Protein A ◽  
Bloodstream Infections ◽  
Outer Membrane Protein A
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