scholarly journals Tissue‐specific variation in nonsense mutant transcript level and drug‐induced read‐through efficiency in the Cln1 R151X mouse model of INCL

2016 ◽  
Vol 20 (2) ◽  
pp. 381-385 ◽  
Author(s):  
Vaughn Thada ◽  
Jake N. Miller ◽  
Attila D. Kovács ◽  
David A. Pearce
Keyword(s):  
Mouse Model ◽  
Transcript Level ◽  
Drug Induced ◽  
Tissue Specific ◽  
Nonsense Mutant ◽  
Mutant Transcript ◽  
Specific Variation

scholarly journals Tissue-specific variation of Ube3a protein expression in rodents and in a mouse model of Angelman syndrome

2010 ◽  
Vol 39 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Richard M. Gustin ◽  
Terry Jo Bichell ◽  
Michael Bubser ◽  
Jennifer Daily ◽  
Irina Filonova ◽  
...  
Keyword(s):  
Mouse Model ◽  
Protein Expression ◽  
Angelman Syndrome ◽  
Tissue Specific ◽  
Specific Variation

A Modified TurboID Approach Identifies Tissue-Specific Centriolar Components In C. elegans

2021 ◽  
Author(s):  
Elisabeth Holzer ◽  
Cornelia Rumpf-Kienzl ◽  
Sebastian Falk ◽  
Alexander Dammermann
Keyword(s):  
Protein Interactions ◽  
Affinity Purification ◽  
Experimental Models ◽  
Protein Protein Interactions ◽  
Tissue Specific ◽  
C Elegans ◽  
Early Embryos ◽  
Somatic Tissues ◽  
Specific Variation ◽  
Labeling Method

Proximity-dependent labeling approaches such as BioID have been a great boon to studies of protein-protein interactions in the context of cytoskeletal structures such as centrosomes which are poorly amenable to traditional biochemical approaches like immunoprecipitation and tandem affinity purification. Yet, these methods have so far not been applied extensively to invertebrate experimental models such as C. elegans given the long labeling times required for the original promiscuous biotin ligase variant BirA*. Here, we show that the recently developed variant TurboID successfully probes the interactomes of both stably associated (SPD-5) and dynamically localized (PLK-1) centrosomal components. We further develop an indirect proximity labeling method employing a GFP nanobody- TurboID fusion, which allows the identification of protein interactors in a tissue-specific manner in the context of the whole animal. Critically, this approach utilizes available endogenous GFP fusions, avoiding the need to generate multiple additional strains for each target protein and the potential complications associated with overexpressing the protein from transgenes. Using this method, we identify homologs of two highly conserved centriolar components, Cep97 and Bld10/Cep135, which are present in various somatic tissues of the worm. Surprisingly, neither protein is expressed in early embryos, likely explaining why these proteins have escaped attention until now. Our work expands the experimental repertoire for C. elegans and opens the door for further studies of tissue-specific variation in centrosome architecture.

scholarly journals Generation of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Hyeongseok Kim ◽  
Yeong Gi Kim ◽  
Wonsuk Choi ◽  
Joon Ho Moon ◽  
Inseon Hwang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Knockout Mouse ◽  
Tryptophan Hydroxylase ◽  
Tissue Specific ◽  
Knockout Mouse Model ◽  
Highly Efficient

scholarly journals Nuclear receptor HNF4A transrepresses CLOCK:BMAL1 and modulates tissue-specific circadian networks

2018 ◽  
Vol 115 (52) ◽  
pp. E12305-E12312 ◽  
Author(s):  
Meng Qu ◽  
Tomas Duffy ◽  
Tsuyoshi Hirota ◽  
Steve A. Kay
Keyword(s):  
Nuclear Receptor ◽  
Metabolic Pathways ◽  
Feedback Loop ◽  
Transcriptional Activity ◽  
Transcript Level ◽  
Colon Cells ◽  
Tissue Specific ◽  
Metabolic Genes ◽  
Genome Wide ◽  
Circadian Control

Either expression level or transcriptional activity of various nuclear receptors (NRs) have been demonstrated to be under circadian control. With a few exceptions, little is known about the roles of NRs as direct regulators of the circadian circuitry. Here we show that the nuclear receptor HNF4A strongly transrepresses the transcriptional activity of the CLOCK:BMAL1 heterodimer. We define a central role for HNF4A in maintaining cell-autonomous circadian oscillations in a tissue-specific manner in liver and colon cells. Not only transcript level but also genome-wide chromosome binding of HNF4A is rhythmically regulated in the mouse liver. ChIP-seq analyses revealed cooccupancy of HNF4A and CLOCK:BMAL1 at a wide array of metabolic genes involved in lipid, glucose, and amino acid homeostasis. Taken together, we establish that HNF4A defines a feedback loop in tissue-specific mammalian oscillators and demonstrate its recruitment in the circadian regulation of metabolic pathways.

LKB1 GENE TISSUE SPECIFIC KNOCKOUT MOUSE MODEL FOR PANCREATIC CANCER

Pancreas ◽  
2005 ◽  
Vol 31 (4) ◽  
pp. 478
Author(s):  
C Wei ◽  
A Rashid ◽  
C Amos ◽  
M Gannon ◽  
M L Frazier
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Knockout Mouse ◽  
Tissue Specific ◽  
Knockout Mouse Model

Tissue-Specific variation in the length of the 5? untranslated region of the ?a-inhibin mRNA in sheep

1995 ◽  
Vol 40 (1) ◽  
pp. 1-8 ◽  
Author(s):  
J. S. Fleming ◽  
S. M. Galloway ◽  
R. J. Crawford ◽  
D. J. Tisdall ◽  
P. J. Greenwood
Keyword(s):  
Untranslated Region ◽  
Tissue Specific ◽  
Specific Variation
Sign in / Sign up

Export Citation Format

Share Document