Effect of culture conditions on conidia production and enhancement of environmental stress resistance of Esteya vermicola in solid‐state fermentation

2020 ◽  
Author(s):  
Y. Zhu ◽  
Y. Mao ◽  
T. Ma ◽  
X. Wen
Keyword(s):  
Solid State ◽  
Environmental Stress ◽  
Solid State Fermentation ◽  
Stress Resistance ◽  
Culture Conditions ◽  
Conidia Production ◽  
Esteya Vermicola

scholarly journals Optimization Of Culture Conditions For The Production Of Xylanase By Two Thermophilic Fungi Under Solid State Fermentation

2013 ◽  
Vol 39 (1) ◽  
pp. 43-51 ◽  
Author(s):  
Kumkum Azad ◽  
Md. Abdul Halim ◽  
Feroza Hossain
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Culture Conditions ◽  
Maximum Activity ◽  
Thermomyces Lanuginosus ◽  
Thermophilic Fungi ◽  
Thermostable Xylanase ◽  
Optimum Time ◽  
Xylanase Production ◽  
Time Required

Two thermophilic fungi, Thermomyces lanuginosus BPJ-10 and Rhizomucor pusillus BPJ-2 were studied under solid state fermentation (SSF) using wheat bran for the production of thermostable xylanase. The optimum time required for the production of xylanase was found to be 4 days and 7 days for R. pusillus BPJ-2 and T. lanuginosus BPJ-10 respectively. The optimum temperatures for the production of xylanase by R. pusillus BPJ-2 and T. lanuginosus BPJ-10 were 45°C and 50°C respectively. The maximum activity of xylanase (1.685 IU/ml and 0.075 IU/ml) was exhibited by T. lanuginosus BPJ-10 and R. pusillus BPJ-2 at pH 7.0 and pH 4.0 respectively. The optimum moisture content for maximum xylanase production was 90% for both fungi. J. Asiat. Soc. Bangladesh, Sci. 39(1): 43-51, June 2013 DOI: http://dx.doi.org/10.3329/jasbs.v39i1.16032

Optimization of Culture Conditions During the Solid-State Fermentation of Tea Residue Using Mixed Strains

2020 ◽  
Vol 11 (12) ◽  
pp. 6667-6675 ◽  
Author(s):  
Xiaoqing Ding ◽  
Lei Yao ◽  
Yong Hou ◽  
Yanbin Hou ◽  
Genliang Wang ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Culture Conditions ◽  
Optimization Of Culture Conditions

scholarly journals Characterization of best naringinase producing fungus isolated from the citrus fruits

2016 ◽  
Vol 4 (2) ◽  
pp. 83 ◽  
Author(s):  
Keerthini Srikantha ◽  
Kapilan Ranganathan ◽  
Vasantharuba Seevaratnam
Keyword(s):  
Solid State ◽  
Aspergillus Flavus ◽  
Solid State Fermentation ◽  
Large Scale ◽  
Citrus Fruit ◽  
Fermentation Medium ◽  
Culture Conditions ◽  
Citrus Fruits ◽  
Biochemical Tests

Naringinase enzyme has potential application in food and pharmaceutical industry. Naringin and limonin are principle bitter components in the citrus fruit. The microorganisms that associate with citrus fruit may have the ability to degrade the naringin by extracellular naringinase enzymes that are produced by microorganisms. The objective of the study is to isolate naringinase producing fungus from the citrus fruit to debitter the citrus juice and to characeterize the fungus. Citrus fruits were allowed to spoil under the air and soil and the lesion was used to streak on fresh PDA plates. Out of the eight strains isolated from citrus fruits, five were positive for naringinase enzyme. When all the naringinase producing fungi were subjected to liquid fermentation medium for eight days at room temperature at 200 rpm and the crude enzyme was tested for naringinase enzyme at pH 5 and 50 ºC for 10 minutes, one strain showed the best naringenase activity (1.92 µmol/ml/min). This strain was identified as Aspergillus flavus based on the macroscopic, microscopic and biochemical tests. The culture conditions were optimized to increase the naringinase production via solid state fermentation system using paddy husk as the support. Though naringinase activity of Aspergillus flavus has started on the 2nd day, the highest activity (449.58Ug-1Dry Matter) was obtained on the 8th day. Thereafter the naringinase activity has started to decline. Solid state fermentation using paddy husk as support could be used for large scale naringinase enzyme production.

scholarly journals Optimization of Culture Conditions for Maximal Lovastatin Production by Aspergillus terreus (KM017963) under Solid State Fermentation

2015 ◽  
Vol 22 (4) ◽  
pp. 174-180 ◽  
Author(s):  
Praveen Vadakke Kamath ◽  
Bhargavi Santebennur Dwarakanath ◽  
Avinash Chaudhary ◽  
Savitha Janakiraman
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Aspergillus Terreus ◽  
Culture Conditions ◽  
Optimization Of Culture Conditions

scholarly journals PENGARUH KONDISI KULTUR PADA AKTIVITAS SELULASE ISOLAT Pycnoporus sp. DAN Phlebiopsis sp. (EFFECTS OF CULTURE CONDITIONS ON CELLULASE ACTIVITIES PRODUCED BY Pycnoporus sp. AND Phlebiopsis sp.)

2017 ◽  
Vol 7 (02) ◽  
pp. 79
Author(s):  
Luciasih Agustini ◽  
Ragil S.B Irianto ◽  
Maman Turjaman ◽  
Sarah Asih Faulina ◽  
Resti Ariantari ◽  
...  
Keyword(s):  
Solid State ◽  
Rice Bran ◽  
Solid State Fermentation ◽  
Enzyme Activities ◽  
Culture Conditions ◽  
Lignocellulosic Waste ◽  
Corn Cobs ◽  
Fungal Isolates ◽  
Cellulase Complex ◽  
Effects Of Media

The effects of media, pH and temperature on cellulase-complex enzyme produced by Pycnoporus sp. FORDACC-03452 and Phlebiopsis sp. FORDACC-02482 cultivated in rice bran and corn cobs media under solid state fermentation with pH 4–7 and temperature 30°C–45°C were investigated. Rice bran media showed a propensity to induce endo-β,1,4-glucanase and cellobiohydrolase productions, while corn cobs media induce β-glucosidase production. However, the mixture of rice bran and corn cobs did not result in better cellulase complex enzyme activities. Cellulase-complex produced by Pycnoporussp. showed superior activities compared to those produced by Phlebiopsissp. Crude enzyme of Pycnoporus sp. showed optimum specifc-activities of endo-β-1,4-glucanase at pH 6, temperature 35°C (0.403 ± 0.010 IU/mg), cellobiohydrolase at pH 6, temperature 40°C (0.540 ± 0.020 IU/mg) and β-glucosidase at pH 4, temperature 30 °C (0.022 ± 0.001 IU/mg). While Phlebiopsis sp. showed optimum specifc-activities of endo-β-1,4-glucanase at pH 6, temperature 35°C (0.202 ± 0.005 IU/mg), cellobiohydrolase at pH 4, temperature 45°C (0.180 ± 0.002 IU/mg) and β-glucosidase at pH 6, temperature 45°C (0.007 ± 0.001 IU/mg). Due to low β-glucosidase activities, the cellulase-complex generated from this study were not able to completely hydrolyse lignocellulosic waste and yielded unsufficient sugars content. Further investigation to optimize cellulase-complex production from these fungal isolates is still required.ABSTRAKPenelitian pengaruh media kultivasi, pH dan suhu inkubasi terhadap produksi enzim selulase-kompleks dari Pycnoporus sp. FORDACC-03452 dan Phlebiopsis sp. FORDACC-02482 yang ditumbuhkan di media dedak padi dan tongkol jagung dengan metode kultur padat pada variasi pH 4–7 dan suhu 30°C–45°C, telah dilakukan. Hasil memperlihatkan bahwa media dedak padi cenderung menginduksi produksi endo-β-1,4-glukanase dan selobiohidrolase, sedangkan media tongkol jagung menginduksi produksi β-glukosidase. Namun, campuran kedua substrat tersebut tidak menghasilkan aktivitas selulase yang lebih baik. Selulase-kompleks yang dihasilkan Pycnoporus sp. menunjukkan aktivitas lebih baik dibandingkan dengan yang diproduksi Phlebiopsis sp. Filtrat kasar Pycnoporus sp. menunjukkan aktivitas-spesifk endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,403 ± 0,010 IU/mg); selobiohidrolase pada pH 6, suhu 40°C (0,540 ± 0,020 IU/mg); dan β -glukosidase pada pH 4, suhu 30°C (0,022±0,001 IU/mg). Sementara, Phlebiopsis sp. menunjukkan aktivitas-spesifk endo-β-1,4-glukanase optimum pada pH 6, suhu 35°C (0,202 ± 0,005 IU/mg); selobiohidrolase pada pH 4, suhu 45°C (0,180 ± 0,002 IU/mg); dan β-glukosidase pada pH 6, suhu 45°C (0,007 ± 0,001 IU/mg). Rendahnya aktivitas β-glukosidase menyebabkan selulase-kompleks dari penelitian ini belum dapat menghidrolisis limbah lignoselulosa dengan sempurna dan kadar glukosa yang diperoleh masih rendah. Oleh karena itu, optimasi produksi selulasekompleks dari Pycnoporus sp. dan Phlebiopsis sp. masih perlu diteliti lebih lanjut.

scholarly journals Production of inulinase from Rhizopusoryzae by solid state fermentation

2014 ◽  
Vol 8 (4) ◽  
pp. 46-55
Author(s):  
Qays M. Issa ◽  
Muna H. AL-joburi ◽  
Abdul-Kareem J. Hashim
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Reducing Sugars ◽  
Tap Water ◽  
Corn Steep Liquor ◽  
Natural Habitat ◽  
Jerusalem Artichoke ◽  
Culture Conditions ◽  
Saccharum Sp ◽  
Steep Liquor

Fifty Rhizopus spp. isolates were obtained from local natural habitat. The ability of inulinase production by these isolates was screened. The isolate RC-2 which isolated from composite plants was the highest inulinase producer on modified Czapek-Dox agar in primary screening. Secondary screening revealed that the same isolate was the highest production on Nakamura broth medium. Isolate was identified as Rhizopusoryzae. Measurements of reducing sugars in crude filtrate which represent the products of enzyme revealed that Rhizopusoryzae RC-2 produced inulinaseextracellular. The optimum culture conditions for inulinase production by solid state fermentation included a mixture of Jerusalem artichoke Helianthus tuberosus tuber with sugarcane Saccharum sp. bagasse with a ratio (1:1) as carbon source, 2.5% of corn steep liquor as nitrogen source, moisturizing ratio (3:1) (v:w) with tap water, the best inoculums rate was 2.5×105 spore/ml and incubation at (30)ºC for 4 days.

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