Identification of Vibrio alginolyticus virulent strain‐specific DNA regions by suppression subtractive hybridization and PCR

2020 ◽  
Vol 129 (6) ◽  
pp. 1472-1485
Author(s):  
Z.‐Y. Xie ◽  
X.‐X. Gong ◽  
X.‐D. Xu ◽  
B. Mei ◽  
X.‐Z. Xuan ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Virulent Strain ◽  
Subtractive Hybridization ◽  
Vibrio Alginolyticus

scholarly journals Differentially Expressed Genes of Virulent and NonvirulentEntamoeba histolyticaStrains Identified by Suppression Subtractive Hybridization

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Michelle A. R. Freitas ◽  
Ângela C. Alvarenga ◽  
Helen C. Fernandes ◽  
Frederico F. Gil ◽  
Maria N. Melo ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Virulent Strain ◽  
Subtractive Hybridization ◽  
Invasive Disease ◽  
Rt Pcr ◽  
Gene Expressions ◽  
Cysteine Rich Protein ◽  
Differential Gene ◽  
Cdna Fragments ◽  
Insight Into

Entamoeba histolyticais a parasite which presents capacity to degrade tissues and therefore has a pathogenic behavior. As this behavior is not shown by all strains, there have been several studies investigating molecular basis of the cytotoxicity process. Using the suppression subtractive hybridization (SSH) technique, differential gene expressions of twoE. histolyticastrains, one virulent (EGG) and one nonvirulent (452), have been analyzed with the purpose of isolating genes which may be involved with amoebic virulence. Nine cDNA fragments presenting high homology withE. histolyticapreviously sequenced genes were subtracted. Of these, four genes were confirmed by RT-PCR. Two coding for hypothetical proteins, one for a cysteine-rich protein, expressed only in the virulent strain, EGG and another one, coding for grainin 2 protein, exclusive from 452 strain. This study provided new insight into the proteins differences in the virulent and nonvirulentE. histolyticastrains. We believe that further studies with these proteins may prove association of them with tissue damage, providing new perceptions to improve treatment or diagnosis of the invasive disease.

scholarly journals Genomic Diversity of Burkholderia pseudomallei Clinical Isolates: Subtractive Hybridization Reveals a Burkholderia mallei-Specific Prophage in B. pseudomallei 1026b

2004 ◽  
Vol 186 (12) ◽  
pp. 3938-3950 ◽  
Author(s):  
David DeShazer
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Burkholderia Pseudomallei ◽  
Genomic Sequence ◽  
Subtractive Hybridization ◽  
Etiologic Agent ◽  
Polyclonal Antiserum ◽  
Genomic Diversity ◽  
Clinical Isolates ◽  
Immunogold Electron Microscopy ◽  
Burkholderia Mallei

ABSTRACT Burkholderia pseudomallei is the etiologic agent of the disease melioidosis and is a category B biological threat agent. The genomic sequence of B. pseudomallei K96243 was recently determined, but little is known about the overall genetic diversity of this species. Suppression subtractive hybridization was employed to assess the genetic variability between two distinct clinical isolates of B. pseudomallei, 1026b and K96243. Numerous mobile genetic elements, including a temperate bacteriophage designated φ1026b, were identified among the 1026b-specific suppression subtractive hybridization products. Bacteriophage φ1026b was spontaneously produced by 1026b, and it had a restricted host range, infecting only Burkholderia mallei. It possessed a noncontractile tail, an isometric head, and a linear 54,865-bp genome. The mosaic nature of the φ1026b genome was revealed by comparison with bacteriophage φE125, a B. mallei-specific bacteriophage produced by Burkholderia thailandensis. The φ1026b genes for DNA packaging, tail morphogenesis, host lysis, integration, and DNA replication were nearly identical to the corresponding genes in φE125. On the other hand, φ1026b genes involved in head morphogenesis were similar to head morphogenesis genes encoded by Pseudomonas putida and Pseudomonas aeruginosa bacteriophages. Consistent with this observation, immunogold electron microscopy demonstrated that polyclonal antiserum against φE125 reacted with the tail of φ1026b but not with the head. The results presented here suggest that B. pseudomallei strains are genetically heterogeneous and that bacteriophages are major contributors to the genomic diversity of this species. The bacteriophage characterized in this study may be a useful diagnostic tool for differentiating B. pseudomallei and B. mallei, two closely related biological threat agents.

scholarly journals Identification of up-regulated Ras-like GTPase, Rap1b, by suppression subtractive hybridization

2001 ◽  
Vol 60 (6) ◽  
pp. 2129-2141 ◽  
Author(s):  
Sun Lin ◽  
Sumant Chugh ◽  
Xiaomin Pan ◽  
Elisabeth I. Wallner ◽  
Jun Wada ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Subtractive Hybridization
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