Identification of the immune expressed sequence tags of pearl oyster (Pinctada martensii, Dunker 1850) responding to Vibrio alginolyticus challenge by suppression subtractive hybridization

2012 ◽  
Vol 7 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Yanhong Wang ◽  
Dingkun Fu ◽  
Peng Luo ◽  
Xiaocui He
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Expressed Sequence Tags ◽  
Subtractive Hybridization ◽  
Pearl Oyster ◽  
Vibrio Alginolyticus ◽  
Pinctada Martensii ◽  
Expressed Sequence

Development of Expressed Sequence Tags from the Pearl Oyster, Pinctada martensii Dunker

2010 ◽  
Vol 13 (2) ◽  
pp. 275-283 ◽  
Author(s):  
Aimin Wang ◽  
Yan Wang ◽  
Zhifeng Gu ◽  
Sifa Li ◽  
Yaohua Shi ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Pearl Oyster ◽  
Pinctada Martensii ◽  
Expressed Sequence

scholarly journals Isolation of biosynthesis related transcripts of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside from Fallopia multiflora by suppression subtractive hybridization

2014 ◽  
Vol 83 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Wei Zhao ◽  
Shujing Sheng ◽  
Zhongyu Liu ◽  
Di Lu ◽  
Kuanpeng Zhu ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Expressed Sequence Tags ◽  
Digital Gene Expression ◽  
Subtractive Hybridization ◽  
Full Length ◽  
Specific Difference ◽  
Protein Databases ◽  
Organ Specific ◽  
Rapid Amplification ◽  
Expressed Sequence

2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in <em>Fallopia multiflora</em>, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two <em>F. multiflora</em> varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 full-length transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four <em>F. multiflora</em> tissues (root, stem and leaf from Deqing <em>F. multiflora</em> and another root from Chongqing <em>F. multiflora</em>; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.

Identification of Vibrio alginolyticus virulent strain‐specific DNA regions by suppression subtractive hybridization and PCR

2020 ◽  
Vol 129 (6) ◽  
pp. 1472-1485
Author(s):  
Z.‐Y. Xie ◽  
X.‐X. Gong ◽  
X.‐D. Xu ◽  
B. Mei ◽  
X.‐Z. Xuan ◽  
...  
Keyword(s):  
Suppression Subtractive Hybridization ◽  
Virulent Strain ◽  
Subtractive Hybridization ◽  
Vibrio Alginolyticus

Expressed sequence tags 454 sequencing and biomineralization gene expression for pearl sac of the pearl oyster,Pinctada fucata martensii

2013 ◽  
Vol 46 (3) ◽  
pp. 745-758 ◽  
Author(s):  
Xin Zhan ◽  
Zhifeng Gu ◽  
Chengcheng Yu ◽  
Haiyang Wen ◽  
Yaohua Shi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expressed Sequence Tags ◽  
454 Sequencing ◽  
Pearl Oyster ◽  
Pinctada Fucata ◽  
Pearl Sac ◽  
Expressed Sequence

Seasonal expressed sequence tags of rainbow smelt (Osmerus mordax) revealed by subtractive hybridization and the identification of two genes up-regulated during winter

Gene ◽  
2008 ◽  
Vol 424 (1-2) ◽  
pp. 56-62 ◽  
Author(s):  
Robert C. Richards ◽  
John C. Achenbach ◽  
Connie E. Short ◽  
Jennifer Kimball ◽  
Michael E. Reith ◽  
...  
Keyword(s):  
Expressed Sequence Tags ◽  
Subtractive Hybridization ◽  
Osmerus Mordax ◽  
Rainbow Smelt ◽  
Expressed Sequence
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