scholarly journals Characterization of an Enterococcus faecium strain in a murine mastitis model

2020 ◽  
Vol 128 (5) ◽  
pp. 1289-1300
Author(s):  
I.D. Montironi ◽  
M.V. Moliva ◽  
N.A. Campra ◽  
J.M. Raviolo ◽  
G. Bagnis ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Faecium Strain

scholarly journals Genome Sequence of Enterococcus faecium Strain ICIS 96 Demonstrating Intermicrobial Antagonism Associated with Bacteriocin Production

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Tatiana M. Pashkova ◽  
Alexey S. Vasilchenko ◽  
Yuriy A. Khlopko ◽  
Elena E. Kochkina ◽  
Olga L. Kartashova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Antagonistic Activity ◽  
Bacteriocin Production ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Faecium Strain

ABSTRACT We report here the complete genome sequence of Enterococcus faecium strain ICIS 96, which was isolated from the feces of a horse. Bacteriological characterization of strain ICIS 96 revealed the absence of pathogenicity factors, while its spectrum of antagonistic activity was found to be broad, having activities associated with both Gram-positive and Gram-negative bacteria. Analysis of the E. faecium ICIS 96 genome revealed five genes associated with antimicrobial activity (enterocin [ent] A, ent B, lactobin A/cerein 7b, and ent L50 A/B). No genes that correlate with human pathogenicity were identified.

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

1997 ◽  
Vol 41 (8) ◽  
pp. 1805-1807 ◽  
Author(s):  
M G MacKinnon ◽  
M A Drebot ◽  
G J Tyrrell
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecium ◽  
Insertion Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Detection Strategy ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Identification And Characterization ◽  
Faecium Strain

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

Isolation and characterization of large spectrum and multiple bacteriocin-producing Enterococcus faecium strain from raw bovine milk

2014 ◽  
Vol 118 (2) ◽  
pp. 343-355 ◽  
Author(s):  
N. Gaaloul ◽  
O. ben Braiek ◽  
K. Hani ◽  
A. Volski ◽  
M.L. Chikindas ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Bovine Milk ◽  
Isolation And Characterization ◽  
Faecium Strain

Characterization of a novel theta-type plasmid pSM409 of Enterococcus faecium RME isolated from raw milk

Gene ◽  
2021 ◽  
Vol 777 ◽  
pp. 145459
Author(s):  
Tawsif Ahmed Kazi ◽  
Suranjita Mitra ◽  
Bidhan Chandra Mukhopadhyay ◽  
Sukhendu Mandal ◽  
Swadesh Ranjan Biswas
Keyword(s):  
Enterococcus Faecium ◽  
Raw Milk

Molecular cloning, Gene overexpression and Characterization of Glutamate Decarboxylase from Enterococcus faecium DO

LWT ◽  
2021 ◽  
pp. 111699
Author(s):  
Hanieh Yarabbi ◽  
Seyed Ali Mortazavi ◽  
Masoud Yavarmanesh ◽  
Ali Javadmanesh
Keyword(s):  
Molecular Cloning ◽  
Enterococcus Faecium ◽  
Glutamate Decarboxylase ◽  
Gene Overexpression

scholarly journals Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock

2019 ◽  
Vol 98 (11) ◽  
pp. 5892-5899 ◽  
Author(s):  
Yeong Bin Kim ◽  
Kwang Won Seo ◽  
Jong Bo Shim ◽  
Se hyun Son ◽  
Eun Bi Noh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Parent Stock

scholarly journals Emergence of a daptomycin-non-susceptible Enterococcus faecium strain that encodes mutations in DNA repair genes after high-dose daptomycin therapy

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Takashi Matono ◽  
Kayoko Hayakawa ◽  
Risen Hirai ◽  
Akira Tanimura ◽  
Kei Yamamoto ◽  
...  
Keyword(s):  
Dna Repair ◽  
Enterococcus Faecium ◽  
High Dose ◽  
Dna Repair Genes ◽  
Repair Genes ◽  
Faecium Strain

scholarly journals Draft Genome Sequence of Probiotic Enterococcus faecium Strain L-3

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Alena Karaseva ◽  
Anna Tsapieva ◽  
Justin Pachebat ◽  
Alexander Suvorov
Keyword(s):  
Genome Sequence ◽  
Russian Federation ◽  
Enterococcus Faecium ◽  
Draft Genome ◽  
Bacteriocin Production ◽  
Draft Genome Sequence ◽  
Bacteriocin Producer ◽  
Probiotic Preparation ◽  
The Russian Federation ◽  
Faecium Strain

We report here the draft genome sequence of the bacteriocin producer Enterococcus faecium strain L-3, isolated from a probiotic preparation, Laminolact, which is widely used in the Russian Federation. The draft genome sequence is composed of 74 contigs for a total of 2,643,001 bp, with 2,646 coding genes. Five clusters for bacteriocin production were found.

Characterization of Enterococcus faecium mutants resistant to mundticin KS, a class IIa bacteriocin

Microbiology ◽  
2003 ◽  
Vol 149 (10) ◽  
pp. 2901-2908 ◽  
Author(s):  
Youko Sakayori ◽  
Mizuho Muramatsu ◽  
Satoshi Hanada ◽  
Yoichi Kamagata ◽  
Shinichi Kawamoto ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Antimicrobial Agents ◽  
Wild Type Strain ◽  
Unsaturated Fatty Acids ◽  
Class I ◽  
Wild Type ◽  
Food Preservatives ◽  
In The Wild ◽  
Resistant Mutants

The emergence and spread of mutants resistant to bacteriocins would threaten the safety of using bacteriocins as food preservatives. To determine the physiological characteristics of resistant mutants, mutants of Enterococcus faecium resistant to mundticin KS, a class IIa bacteriocin, were isolated. Two types of mutant were found that had different sensitivities to other antimicrobial agents such as nisin (class I) and kanamycin. Both mutants were resistant to mundticin KS even in the absence of Mg2+ ions. The composition of unsaturated fatty acids in the resistant mutants was significantly increased in the presence of mundticin KS. The composition of the phospholipids in the two resistant mutants also differed from those in the wild-type strain. The putative zwitterionic amino-containing phospholipid in both mutants significantly increased, whereas amounts of phosphatidylglycerol and cardiolipin decreased. These changes in membrane structure may influence resistance of enterococci to class IIa and class I bacteriocins.

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