Isolation and characterization of large spectrum and multiple bacteriocin-producing Enterococcus faecium strain from raw bovine milk

2014 ◽  
Vol 118 (2) ◽  
pp. 343-355 ◽  
Author(s):  
N. Gaaloul ◽  
O. ben Braiek ◽  
K. Hani ◽  
A. Volski ◽  
M.L. Chikindas ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Bovine Milk ◽  
Isolation And Characterization ◽  
Faecium Strain

Isolation and characterization of two new Staphylococcus aureus bacteriophages with potential to infect distinct bacteria from bovine mastitis

2021 ◽  
Author(s):  
Bibiana Martins Barasuol ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Antônio Sangioni ◽  
Daniela Isabel Brayer Pereira ◽  
Ana Paula Muterle Varela ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Bovine Milk ◽  
Bovine Mastitis ◽  
Gc Content ◽  
Etiologic Agent ◽  
Cure Rate ◽  
Long Tail ◽  
Isolation And Characterization ◽  
Double Stranded Dna

Abstract Bovine mastitis is an important disease of dairy cows, and Staphylococcus aureus is the etiologic agent most prevalent among the microorganisms. Mastitis caused by S. aureus present low cure rate with antimicrobials treatment and low vaccines efficacy. Bacteriophages or phages have been considered as an alternative for treating this disease. This study, we isolated and characterized two new S. aureus phages, namely B_UFSM4 and B_UFSM5, from bovine milk of cows with mastitis. The adsorptions rates were 10–20 min for B_UFSM4 and 20–30 min for B_UFSM5. Phages activities were relatively stable at pH 3–11; however, at temperatures of 50 °C-60ºC-70ºC/60 min, the phages were completely inactivated. These viruses presented infectivity in various bacteria isolated from bovine mastitis, where the lytic activity of phages B_UFSM4 and B_UFSM5 were 34.2%(13/38) and 42.1%(16/38), respectively, including isolates from S. aureus, Pseudomonas aeruginosa, Staphylococcus sciuri, and Rothia terrae. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, with GC-content 33.97% and 33.98%, respectively. Both phages comprise 61 putative ORFs. The viruses have double stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus prophage phiPV83 (45,536 nt), Staphylococcus phage CN125 (44,492 nt) and Staphylococcus phage JS01 (43,458 nt). Based on the morphology, the phages belong to Siphoviridae family, presenting icosahedral head with a long tail, Caudovirales order and Biseptimavirus genus. Thus, two S. aureus phages (B_UFSM4 and B_UFSM5) were isolated and characterized, and these phages can be used as therapeutic or prophylactic candidates against S. aureus infections in cattle mastitis.

Isolation and characterization of Enterococcus faecium strains for calcium l-lactate production

2012 ◽  
Vol 29 ◽  
pp. S56
Author(s):  
E. Oltuszak-Walczak ◽  
P. Walczak ◽  
P. Pietraszek ◽  
A. Otlewska ◽  
K. Dybka ◽  
...  
Keyword(s):  
Enterococcus Faecium ◽  
Lactate Production ◽  
Isolation And Characterization

Isolation and characterization of plasminogen and plasmin from bovine milk

1995 ◽  
Vol 5 (6) ◽  
pp. 577-592 ◽  
Author(s):  
Connie Benfeldt ◽  
Lotte B. Larsen ◽  
Jan T. Rasmussen ◽  
Peter A. Andreasen ◽  
Torben E. Petersen
Keyword(s):  
Bovine Milk ◽  
Isolation And Characterization

scholarly journals Genome Sequence of Enterococcus faecium Strain ICIS 96 Demonstrating Intermicrobial Antagonism Associated with Bacteriocin Production

2018 ◽  
Vol 6 (10) ◽  
Author(s):  
Tatiana M. Pashkova ◽  
Alexey S. Vasilchenko ◽  
Yuriy A. Khlopko ◽  
Elena E. Kochkina ◽  
Olga L. Kartashova ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Genome Sequence ◽  
Enterococcus Faecium ◽  
Antagonistic Activity ◽  
Bacteriocin Production ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Content Type ◽  
Faecium Strain

ABSTRACT We report here the complete genome sequence of Enterococcus faecium strain ICIS 96, which was isolated from the feces of a horse. Bacteriological characterization of strain ICIS 96 revealed the absence of pathogenicity factors, while its spectrum of antagonistic activity was found to be broad, having activities associated with both Gram-positive and Gram-negative bacteria. Analysis of the E. faecium ICIS 96 genome revealed five genes associated with antimicrobial activity (enterocin [ent] A, ent B, lactobin A/cerein 7b, and ent L50 A/B). No genes that correlate with human pathogenicity were identified.

Identification and characterization of IS1476, an insertion sequence-like element that disrupts VanY function in a vancomycin-resistant Enterococcus faecium strain.

1997 ◽  
Vol 41 (8) ◽  
pp. 1805-1807 ◽  
Author(s):  
M G MacKinnon ◽  
M A Drebot ◽  
G J Tyrrell
Keyword(s):  
Resistance Gene ◽  
Enterococcus Faecium ◽  
Insertion Sequence ◽  
Vancomycin Resistant Enterococcus ◽  
Detection Strategy ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Identification And Characterization ◽  
Faecium Strain

The vanY gene of vancomycin-resistant enterococci encodes a D,D-carboxypeptidase. By using a PCR detection strategy, a VanA Enterococcus faecium clinical isolate was found to have an insertion sequence (IS)-like element designated IS1476 in vanY. The activity of the VanY D,D-carboxypeptidase in this isolate was decreased in a fluorometric fluoraldehyde o-phthalaldehyde assay with diacetyl-L-Lys-D-Ala-D-Ala as the substrate. This, to our knowledge, is the first report of an IS-like element in a vancomycin resistance gene.

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