scholarly journals Matrix approach to the simultaneous detection of multiple potato pathogens by real-time PCR

2018 ◽  
Vol 124 (3) ◽  
pp. 797-809 ◽  
Author(s):  
M.M. Nikitin ◽  
N.V. Statsyuk ◽  
P.A. Frantsuzov ◽  
V.G. Dzhavakhiya ◽  
A.G. Golikov
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Matrix Approach ◽  
Potato Pathogens

Simultaneous detection of 45 fusion genes in leukemia by dual-color fluorescence real-time PCR

2017 ◽  
Vol 39 (2) ◽  
pp. 175-184
Author(s):  
Z. Zheng ◽  
P. Zhang ◽  
G. He ◽  
K. Liao ◽  
Z. Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Fusion Genes ◽  
Dual Color

Simultaneous Detection of Selected Fungal and Bacterial Pathogens by Real Time PCR in Patients with Fever and Neutropenia

2018 ◽  
Vol 18 ◽  
pp. S186
Author(s):  
Mervat Mansour ◽  
Iman Ragab ◽  
Marwa Saad ◽  
Eman Elkholy ◽  
Ebtsam Abdel-Hafez
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection

scholarly journals Development and application of a triplex real-time PCR assay for simultaneous detection of avian influenza virus, Newcastle disease virus, and duck Tembusu virus

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Xiyu Zhang ◽  
Ming Yao ◽  
Zhihui Tang ◽  
Daning Xu ◽  
Yan Luo ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Standard Deviation ◽  
Real Time ◽  
Real Time Pcr ◽  
Virus Isolation ◽  
Simultaneous Detection ◽  
Conventional Pcr ◽  
Pcr Assay ◽  
Duck Tembusu Virus ◽  
Relative Standard

Abstract Background Pathogens including duck-origin avian influenza virus (AIV), duck-origin Newcastle disease virus (NDV) and duck Tembusu virus (DTMUV) posed great harm to ducks and caused great economic losses to the duck industry. In this study, we aim to develop a triplex real-time polymerase chain reaction (PCR) assay to detect these three viruses as early as possible in the suspicious duck flocks. Results The detection limit of the triplex real-time PCR for AIV, NDV, and DTMUV was 1 × 101 copies/μL, which was at least 10 times higher than the conventional PCR. In addition, the triplex assay was highly specific, and won’t cross-react with other duck pathogens. Besides, the intra-day relative standard deviation and inter-day relative standard deviation were lower than 4.44% for these viruses at three different concentrations. Finally, a total of 120 clinical samples were evaluated by the triplex real-time PCR, the conventional PCR and virus isolation, and the positive rates for these three methods were 20.83, 21.67, 19.17%, respectively. Taking virus isolation as the gold standard, the diagnostic specificity and positive predictive value of the three viruses were all above 85%, while the diagnostic sensitivity and negative predictive value of the three viruses were all 100%. Conclusion The developed triplex real-time PCR is fast, specific and sensitive, and is feasible and effective for the simultaneous detection of AIV, NDV, and DTMUV in ducks.

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