Simultaneous Detection of Selected Fungal and Bacterial Pathogens by Real Time PCR in Patients with Fever and Neutropenia

2018 ◽  
Vol 18 ◽  
pp. S186
Author(s):  
Mervat Mansour ◽  
Iman Ragab ◽  
Marwa Saad ◽  
Eman Elkholy ◽  
Ebtsam Abdel-Hafez
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection

scholarly journals Development and application of two multiplex real-time PCR assays for detection and speciation of bacterial pathogens in the koala

2018 ◽  
Vol 30 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Lyndal S. Hulse ◽  
Danica Hickey ◽  
Jessica M. Mitchell ◽  
Kenneth W. Beagley ◽  
William Ellis ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Turnaround Time ◽  
Clinical Samples ◽  
Phascolarctos Cinereus ◽  
In The Wild ◽  
Labeled Probe ◽  
Free Ranging

Infectious diseases have contributed to the decline in the health of koala ( Phascolarctos cinereus) populations in the wild in some regions of Australia. Herein we report the development and validation of 2 multiplex real-time PCR (rtPCR) panels for the simultaneous detection of Mycoplasma spp., Ureaplasma spp., Bordetella bronchiseptica, and Chlamydia, including speciation and quantification of Chlamydia, in ocular, reproductive, and nasal swab samples in addition to semen and male urogenital and reproductive tissues, from koalas. Each rtPCR panel was developed for use as a single-tube reaction using pathogen-specific primers and fluorescently labeled probe sets. DNA extracted from reference strains and isolates was used for validation of sequence gene targets for the multiplex rtPCR panels. Each panel was shown to be sensitive and specific in detecting and differentiating the bacterial pathogens. The multiplex rtPCR panels were used to screen clinical samples from free-ranging and hospitalized koalas for multiple pathogens simultaneously. The multiplex rtPCR will improve turnaround time compared to individual-pathogen rtPCR methods used, to date, for confirmation of diagnosis and will provide the wildlife clinician with the ability to make treatment decisions more rapidly.

Simultaneous detection of 45 fusion genes in leukemia by dual-color fluorescence real-time PCR

2017 ◽  
Vol 39 (2) ◽  
pp. 175-184
Author(s):  
Z. Zheng ◽  
P. Zhang ◽  
G. He ◽  
K. Liao ◽  
Z. Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Simultaneous Detection ◽  
Fusion Genes ◽  
Dual Color

scholarly journals Detection of Uncommon Enteric Bacterial Pathogens from Acute Diarrheal Specimens Using SYBR-Green Real Time PCR

2019 ◽  
Vol 72 (2) ◽  
pp. 88-93 ◽  
Author(s):  
Goutam Chowdhury ◽  
Oscar C. Stine ◽  
Krishnan Rajendran ◽  
Asish K. Mukhopadhyay ◽  
Keinosuke Okamoto ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Bacterial Pathogens ◽  
Sybr Green
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