Polyhydroxyalkanoate (PHA) accumulation potential and PHA-accumulating microbial communities in various activated sludge processes of municipal wastewater treatment plants

2014 ◽  
Vol 118 (1) ◽  
pp. 255-266 ◽  
Author(s):  
K. Sakai ◽  
S. Miyake ◽  
K. Iwama ◽  
D. Inoue ◽  
S. Soda ◽  
...  
1994 ◽  
Vol 30 (4) ◽  
pp. 211-214 ◽  
Author(s):  
E. Brands ◽  
M. Liebeskind ◽  
M. Dohmann

This study shows a comparison of important parameters for dynamic simulation concerning the highrate and low-rate activated sludge tanks of several municipal wastewater treatment plants. The parameters for the dynamic simulation of the single-stage process are quite well known, but parameters for the high-ratellow-rate activated sludge process are still missi ng, although a considerable number of wastewater treatment plants are designed and operated that way. At present any attempt to simulate their operation is restricted to the second stage due to missing data concerning growth rate, decay rate, yield coefficient and others.


1998 ◽  
Vol 38 (1) ◽  
pp. 303-310 ◽  
Author(s):  
V. Naidoo ◽  
V. Urbain ◽  
C. A. Buckley

Denitrification kinetics and wastewater characterization of eight different plants in Europe are discussed. Denitrification batch tests revealed three distinct rates except in the cases of Plaisir, Rostock and Orense where 4 rates were observed. The latter three plants revealed atypical rapid initial rates which were between 7 and 21 mgN/gVSS.h. All denitrification kinetics under non-limiting carbon conditions revealed fast first rates which ranged between 3.0 and 7.3 mgN/gVSS.h. Acetate was used to simulate denitrification kinetics with readily biodegradable COD present. Two subsequent rates were observed. Rates 2 and 3 ranged between 2 and 3 mgN/gVSS.h, and 1 and 2 mgN/gVSS.h, respectively. The RBCOD fraction varied between 10 and 19%, except for one of the plants where the value determined was 7%.


2019 ◽  
Vol 86 (4) ◽  
Author(s):  
Yang Song ◽  
Cheng-Ying Jiang ◽  
Zong-Lin Liang ◽  
Bao-Jun Wang ◽  
Yong Jiang ◽  
...  

ABSTRACT Microorganisms in wastewater treatment plants (WWTPs) play a key role in the removal of pollutants from municipal and industrial wastewaters. A recent study estimated that activated sludge from global municipal WWTPs harbors 1 × 109 to 2 × 109 microbial species, the majority of which have not yet been cultivated, and 28 core taxa were identified as “most-wanted” ones (L. Wu, D. Ning, B. Zhang, Y. Li, et al., Nat Microbiol 4:1183–1195, 2019, https://doi.org/10.1038/s41564-019-0426-5). Cultivation and characterization of the “most-wanted” core bacteria are critical to understand their genetic, physiological, phylogenetic, and ecological traits, as well as to improve the performance of WWTPs. In this study, we isolated a bacterial strain, designated SJ-1, that represents a novel cluster within Betaproteobacteria and corresponds to OTU_16 within the 28 core taxa in the “most-wanted” list. Strain SJ-1 was identified and nominated as Casimicrobium huifangae gen. nov., sp. nov., of a novel family, Casimicrobiaceae. C. huifangae is ubiquitously distributed and is metabolically versatile. In addition to mineralizing various carbon sources (including carbohydrates, aromatic compounds, and short-chain fatty acids), C. huifangae is capable of nitrate reduction and phosphorus accumulation. The population of C. huifangae accounted for more than 1% of the bacterial population of the activated sludge microbiome from the Qinghe WWTP, which showed seasonal dynamic changes. Cooccurrence analysis suggested that C. huifangae was an important module hub in the bacterial network of Qinghe WWTP. IMPORTANCE The activated sludge process is the most widely applied biotechnology and is one of the best ecosystems to address microbial ecological principles. Yet, the cultivation of core bacteria and the exploration of their physiology and ecology are limited. In this study, the core and novel bacterial taxon C. huifangae was cultivated and characterized. This study revealed that C. huifangae functioned as an important module hub in the activated sludge microbiome, and it potentially plays an important role in municipal wastewater treatment plants.


2008 ◽  
Vol 58 (8) ◽  
pp. 1671-1677 ◽  
Author(s):  
A. F. van Nieuwenhuijzen ◽  
A. G. N. van Bentem ◽  
A. Buunnen ◽  
B. A. Reitsma ◽  
C. A. Uijterlinde

The (low loaded) biological nutrient removing activated sludge process is the generally accepted and applied municipal wastewater treatment method in the Netherlands. The hydraulical and biological flexibility, robustness and cost efficiency of the process for advanced removal of nutrients like nitrogen and phosphorus without (too much) chemicals results in a wide application of the activated sludge process within Dutch waterboards. Presumably, wastewater treatment plants will have to contribute to the improvement of the quality of the receiving surface waters by producing cleaner effluent. In this perspective, the Dutch research organisation STOWA initiated a research project entitled “The Boundaries of the Activated Sludge Process” to investigate the possibilities and limitations of activated sludge processes to improve the effluent quality. It is concluded that the activated sludge process as applied and operated at WWTP's in the Netherlands has the potential to perform even better than the current effluent discharge standards (10 mg Ntotal/l and 1 mg Ptotal/l). Reaching the B-quality effluent (<5mg Ntotal/l and <0.3 mg Ptotal/l) will be possible at almost all WWTPs without major adjustments under the conditions that:   the sludge load is below 0.06 kg BOD/kg TSS.d   the internal recirculation is above 20   the BOD/N ratio of the influent is above 3. Complying with the A-quality effluent (<2.2 Ntotal/l and <0.15 mg Ptotal/l) seems to be difficult (but not impossible) and requires more attention and insight into the activated sludge process. Optimisation measures to reach the A-quality effluent are more thorough and are mostly only achievable by additional construction works (addition of activated sludge volume, increasing recirculation capacity, etc.). It is furthermore concluded that the static HSA-results are comparable to the dynamic ASM-results. So, for fast determinations of the limits of technology of different activated sludge processes static modelling seems to by sufficient.


Water ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 852 ◽  
Author(s):  
Ning Xie ◽  
Liping Zhong ◽  
Liao Ouyang ◽  
Wang Xu ◽  
Qinghuai Zeng ◽  
...  

Municipal wastewater treatment plants (WWTPs) use functional microorganisms in activated sludge (AS) to reduce the environmental threat posed by wastewater. In this study, Illumina NovaSeq sequencing of 16S rRNA genes was performed to explore the microbial communities of AS at different stages of the two WWTP projects in Shenzhen, China. Results showed that Proteobacteria, Bacteroidetes, Acidobacteria, Firmicutes, and Nitrospirae were the dominant phyla in all the samples, with Proteobacteria being the most abundant and reaching a maximum proportion of 59.63%. There was no significant difference in biodiversity between the two water plants, but Stage 1 and Stage 2 were significantly different. The Mantel test indicated that nitrate, total nitrogen (TN), chemical oxygen demand (COD), and nutrients were essential factors affecting the bacterial community structure. FAPROTAX analysis emphasized that the leading functional gene families include nitrification, aerobic nitrite oxidation, human pathogens, and phototrophy. This study reveals changes in the community structure of AS in different treatment units of Banxuegang WWTP, which can help engineers to optimize the wastewater treatment process.


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