scholarly journals Assessing human B cell repertoire diversity and convergence

2018 ◽  
Vol 284 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Katharina Imkeller ◽  
Hedda Wardemann
Keyword(s):  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Repertoire Diversity ◽  
Human B Cell

scholarly journals When designing vaccines, consider the starting material: the human B cell repertoire

2018 ◽  
Vol 53 ◽  
pp. 209-216 ◽  
Author(s):  
Colin Havenar-Daughton ◽  
Robert K. Abbott ◽  
William R. Schief ◽  
Shane Crotty
Keyword(s):  
B Cell ◽  
Starting Material ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Human B Cell

Fas-dependent and Fas-independent Mechanisms for Selection of the Mature Human B Cell Repertoire

1997 ◽  
Vol 815 (1 B-Lymphocytes) ◽  
pp. 67-73 ◽  
Author(s):  
THIERRY DEFRANCE ◽  
GISÈLE BILLIAN ◽  
PETER H. KRAMMER ◽  
CHANTAL LAGRESLE
Keyword(s):  
B Cell ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Human B Cell ◽  
Selection Of

scholarly journals Inferring processes underlying B-cell repertoire diversity

2015 ◽  
Vol 370 (1676) ◽  
pp. 20140243 ◽  
Author(s):  
Yuval Elhanati ◽  
Zachary Sethna ◽  
Quentin Marcou ◽  
Curtis G. Callan ◽  
Thierry Mora ◽  
...  
Keyword(s):  
B Cell ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Statistical Properties ◽  
Cell Repertoire ◽  
Vdj Recombination ◽  
Site Model ◽  
Human B Cells ◽  
B Cell Repertoire ◽  
Repertoire Diversity

We quantify the VDJ recombination and somatic hypermutation processes in human B cells using probabilistic inference methods on high-throughput DNA sequence repertoires of human B-cell receptor heavy chains. Our analysis captures the statistical properties of the naive repertoire, first after its initial generation via VDJ recombination and then after selection for functionality. We also infer statistical properties of the somatic hypermutation machinery (exclusive of subsequent effects of selection). Our main results are the following: the B-cell repertoire is substantially more diverse than T-cell repertoires, owing to longer junctional insertions; sequences that pass initial selection are distinguished by having a higher probability of being generated in a VDJ recombination event; somatic hypermutations have a non-uniform distribution along the V gene that is well explained by an independent site model for the sequence context around the hypermutation site.

scholarly journals Inferring processes underlying B-cell repertoire diversity.

2015 ◽  
Author(s):  
Yuval Elhanati ◽  
Zachary Sethna ◽  
Quentin Marcou ◽  
Curtis G Callan ◽  
Thierry Mora ◽  
...  
Keyword(s):  
B Cell ◽  
Somatic Hypermutation ◽  
Cell Receptor ◽  
Statistical Properties ◽  
Cell Repertoire ◽  
Vdj Recombination ◽  
Site Model ◽  
Human B Cells ◽  
B Cell Repertoire ◽  
Repertoire Diversity

We quantify the VDJ recombination and somatic hypermutation processes in human B-cells using probabilistic inference methods on high-throughput DNA sequence repertoires of human B-cell receptor heavy chains. Our analysis captures the statistical properties of the naive repertoire, first after its initial generation via VDJ recombination and then after selection for functionality. We also infer statistical properties of the somatic hypermutation machinery (exclusive of subsequent effects of selection). Our main results are the following: the B-cell repertoire is substantially more diverse than T-cell repertoires, due to longer junctional insertions; sequences that pass initial selection are distinguished by having a higher probability of being generated in a VDJ recombination event; somatic hypermutations have a non-uniform distribution along the V gene that is well explained by an independent site model for the sequence context around the hypermutation site.

scholarly journals Functionally Convergent B Cell Receptor Sequences in Transgenic Rats Expressing a Human B Cell Repertoire in Response to Tetanus Toxoid and Measles Antigens

2017 ◽  
Vol 8 ◽  
Author(s):  
Jean-Philippe Bürckert ◽  
Axel R. S. X. Dubois ◽  
William J. Faison ◽  
Sophie Farinelle ◽  
Emilie Charpentier ◽  
...  
Keyword(s):  
B Cell ◽  
Tetanus Toxoid ◽  
Cell Receptor ◽  
B Cell Receptor ◽  
Transgenic Rats ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Human B Cell

scholarly journals Clonal Analysis of the Human B-Cell Repertoire Using a Heteromobility Assay

2000 ◽  
Vol 7 (3) ◽  
pp. 507-509 ◽  
Author(s):  
Deepanker Tewari
Keyword(s):  
B Cells ◽  
Genetic Variability ◽  
B Cell ◽  
Clonal Analysis ◽  
Dna Probe ◽  
Cell Repertoire ◽  
B Cell Repertoire ◽  
Cell Clonality ◽  
Human B Cell

ABSTRACT A heteromobility duplex tracking assay was developed to analyze B-cell clonality. The assay was based on the genetic variability of B-cell immunoglobulin (Ig) sequences. Binding of amplified (Ig) sequences to a single-stranded radiolabeled Ig DNA probe resulted in the formation of heteroduplexes. The mobilities of these heteroduplexes helped to distinguish clonal B cells.

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