scholarly journals Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type

Immunology ◽  
2014 ◽  
Vol 143 (3) ◽  
pp. 462-477 ◽  
Author(s):  
Birte S. Steiniger ◽  
Verena Wilhelmi ◽  
Anja Seiler ◽  
Katrin Lampp ◽  
Vitus Stachniss
Keyword(s):  
Dendritic Cells ◽  
Stromal Cells ◽  
Stromal Cell ◽  
White Pulp ◽  
Follicular Dendritic Cells ◽  
Cell Type ◽  
Reticulum Cells ◽  
Splenic White Pulp ◽  
Follicular Dendritic

scholarly journals Anatomy of germinal centers in mouse spleen, with special reference to "follicular dendritic cells"

1978 ◽  
Vol 77 (1) ◽  
pp. 148-164 ◽  
Author(s):  
LL Chen ◽  
JC Adams ◽  
RM Steinman
Keyword(s):  
Dendritic Cells ◽  
De Novo ◽  
Sheep Erythrocyte ◽  
Germinal Centers ◽  
Mouse Spleen ◽  
White Pulp ◽  
Follicular Dendritic Cells ◽  
Thorium Dioxide ◽  
Colloidal Carbon ◽  
Follicular Dendritic

Lymphocyte proliferation in germinal centers (GC's) is thought to be triggered by antigen retained extracellularly on the surface of special "dendritic" cells. The anatomy and function of these cells have not been studied directly or in detail. We therefore examined mouse spleen GC's developing in response to sheep erythrocyte stimulation. We found that distincitve "follicular dendritic cells" (FDC's) were present in both the GC and adjacent mantle region of secondary follicles. The large, irregularly shaped nucleus, containing little heterochromatin, allowed for the light microscope (LM) identification of FDC's. By EM, the cell was stellate in shape sending out long, thin sheets of cytoplasm which could fold and coil into complex arrays. The processes were coated extracellularly by an amorphous electron-dense material of varying thickness, as well as particulates including variable numbers of virions. The FDC cytoplasm lacked organelles of active secretory and endocytic cells, such as well-developed rough endoplasmic reticulum (RER) and lysosomes. These anatomical features readily distinguished FDC's from other cell types, even those that were extended in shape. To pursue these descriptive findings, we injected three electron-dense tracers i.v. and sacrificed the mice 1 h-10 days thereafter. Colloidal carbon, colloidal thorium dioxide (cThO2), and soluble horseradish peroxidase (HRP) were actively sequestered into the vacuolar system of macrophages but were interiorized only in trace amounts by FDC's. Therefore, FDC's are not macrophages by cytologic and functional criteria. FDC's did display a unique property. Both colloidal carbon and thorium dioxide, which are nonimmunogens, could be visualized extracellularly on the cell surface for several days. The meaning of this is unclear, but the association of colloid with FDC's appeared to slow the movement of particulates through the extracellular space into the GC proper. FDC's were not readily identified in splenic white pulp lacking GC's. They must develop de novo then, possibly from novel dendritic cells that we have identified in vitro (Steinman, R. M., and Z. A. Cohn. 1973. J. Exp. Med. 137:1142-1162).

scholarly journals Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 585-590 ◽  
Author(s):  
MR Parwaresch ◽  
HJ Radzun ◽  
ML Hansmann ◽  
KP Peters
Keyword(s):  
Monoclonal Antibody ◽  
Dendritic Cells ◽  
Specific Antigen ◽  
Electron Microscopic Level ◽  
Mononuclear Leukocytes ◽  
Immunoperoxidase Method ◽  
Follicular Dendritic Cells ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

Ki-M4, a new IgG3 monoclonal antibody, selectively recognizes dendritic reticulum cells (DRC; follicular dendritic cells) in all human lymphatic organs, as tested by the immunoperoxidase method on the light and electron microscopic level. This antibody was raised against separated lysosomes of the 12–0-tetradecanoyl phorbol-13-acetate (TPA) stimulated permanent cell line, U-937, derived from a human histiocytic lymphoma. No cross-reactivity was encountered in epithelial and mesenchymal cells, including macrophages and other cells detectable in bronchial and peritoneal lavages. In the nonadherent fraction of the mononuclear blood cells collected from the interphase of a Ficoll- Urografin gradient (density = 1.077 g/ml), 0.1 per million of the cells hitherto not classified as monocytes or lymphocytes showed a strong reaction. All other separated blood cell types were devoid of any reactivity. The observation that DRC share a highly restricted, and thus specific, antigen with a small but distinct subpopulation of mononuclear leukocytes implies their blood derivation.

scholarly journals Follicular Dendritic Cells Are Related to Bone Marrow Stromal Cell Progenitors and to Myofibroblasts

2006 ◽  
Vol 177 (1) ◽  
pp. 280-289 ◽  
Author(s):  
Raquel Muñoz-Fernández ◽  
Francisco Javier Blanco ◽  
Cecilia Frecha ◽  
Francisco Martín ◽  
Maria Kimatrai ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Dendritic Cells ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Follicular Dendritic Cells ◽  
Marrow Stromal Cell ◽  
Follicular Dendritic

scholarly journals Monoclonal antibody Ki-M4 specifically recognizes human dendritic reticulum cells (follicular dendritic cells) and their possible precursor in blood

Blood ◽  
1983 ◽  
Vol 62 (3) ◽  
pp. 585-590 ◽  
Author(s):  
MR Parwaresch ◽  
HJ Radzun ◽  
ML Hansmann ◽  
KP Peters
Keyword(s):  
Monoclonal Antibody ◽  
Dendritic Cells ◽  
Specific Antigen ◽  
Electron Microscopic Level ◽  
Mononuclear Leukocytes ◽  
Immunoperoxidase Method ◽  
Follicular Dendritic Cells ◽  
Reticulum Cells ◽  
Dendritic Reticulum Cells ◽  
Follicular Dendritic

Abstract Ki-M4, a new IgG3 monoclonal antibody, selectively recognizes dendritic reticulum cells (DRC; follicular dendritic cells) in all human lymphatic organs, as tested by the immunoperoxidase method on the light and electron microscopic level. This antibody was raised against separated lysosomes of the 12–0-tetradecanoyl phorbol-13-acetate (TPA) stimulated permanent cell line, U-937, derived from a human histiocytic lymphoma. No cross-reactivity was encountered in epithelial and mesenchymal cells, including macrophages and other cells detectable in bronchial and peritoneal lavages. In the nonadherent fraction of the mononuclear blood cells collected from the interphase of a Ficoll- Urografin gradient (density = 1.077 g/ml), 0.1 per million of the cells hitherto not classified as monocytes or lymphocytes showed a strong reaction. All other separated blood cell types were devoid of any reactivity. The observation that DRC share a highly restricted, and thus specific, antigen with a small but distinct subpopulation of mononuclear leukocytes implies their blood derivation.

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