scholarly journals Human decidual stromal cells secrete C-X-C motif chemokine 13, express B cell-activating factor and rescue B lymphocytes from apoptosis: distinctive characteristics of follicular dendritic cells

2012 ◽  
Vol 27 (9) ◽  
pp. 2775-2784 ◽  
Author(s):  
R. Munoz-Fernandez ◽  
A. Prados ◽  
E. Leno-Duran ◽  
A. Blazquez ◽  
J.- R. Garcia-Fernandez ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
B Cell ◽  
B Lymphocytes ◽  
Stromal Cells ◽  
Follicular Dendritic Cells ◽  
B Cell Activating Factor ◽  
Follicular Dendritic

scholarly journals Follicular dendritic cells inhibit apoptosis in human B lymphocytes by a rapid and irreversible blockade of preexisting endonuclease.

1995 ◽  
Vol 181 (6) ◽  
pp. 1985-1995 ◽  
Author(s):  
E Lindhout ◽  
A Lakeman ◽  
C de Groot
Keyword(s):  
Dendritic Cells ◽  
B Cells ◽  
B Cell ◽  
B Lymphocytes ◽  
Cell Cultures ◽  
Germinal Center ◽  
Follicular Dendritic Cells ◽  
Endonuclease Activity ◽  
Cd40 Ligation ◽  
Follicular Dendritic

During germinal center reactions, a minority of B lymphocytes are selected after successful binding to follicular dendritic cells (FDCs). The majority of the B cells, however, die by apoptosis. One of the characteristics of apoptosis is rapid fragmentation of DNA by an endogenous endonuclease. The regulation of apoptosis and endonuclease activity in germinal center (GC) B cells is largely unknown. In this study we have investigated the induction and inhibition of endonuclease activity in GC B cells. We also investigated the role of FDCs, surface Ig (sIg), sIgM, CD21, CD22 CD40, and intracellular Zn2+ in the regulation of endonuclease activity. We have found that DNA fragmentation in GC B cells is caused by a preexisting endonuclease very similar to NUC-18 (an 18-kD endonuclease identified in rat thymocytes). Endonuclease activity in GC B cells appears to be rapidly and irreversibly blocked after interaction with FDCs, but not after cross-linkage of sIg, sIgM, CD21, CD22, or CD40. Addition of soluble CD40-human IgM fusion protein (sCD40) to FDC-B cell cultures also did not interfere with FDC-mediated B cell rescue. Chelation of intracellular Zn2+ during FDC-B cell cultures resulted in abrogated B cell rescue. These data suggest that FDCs inhibit apoptosis in GC B cells by a rapid inactivation of preexisting endonuclease using a mechanism distinct from CD40 ligation.

scholarly journals Germinal Center Initiation, Variable Gene Region Hypermutation, and Mutant B Cell Selection without Detectable Immune Complexes on Follicular Dendritic Cells

2000 ◽  
Vol 192 (7) ◽  
pp. 931-942 ◽  
Author(s):  
Lynn G. Hannum ◽  
Ann M. Haberman ◽  
Shannon M. Anderson ◽  
Mark J. Shlomchik
Keyword(s):  
Dendritic Cells ◽  
Immune Responses ◽  
B Cell ◽  
Immune Complexes ◽  
Germinal Center ◽  
Serum Antibody ◽  
Cell Formation ◽  
Immunoglobulin M ◽  
Follicular Dendritic Cells ◽  
Follicular Dendritic

Serum antibody (Ab) can play several roles during B cell immune responses. Among these is to promote the deposition of immune complexes (ICs) on follicular dendritic cells (FDCs). ICs on FDCs are generally thought to be critical for normal germinal center (GC) formation and the development and selection of memory B cells. However, it has been very difficult to test these ideas. To determine directly whether FDC-bound complexes do indeed function in these roles, we have developed a transgenic (Tg) mouse in which all B lymphocytes produce only the membrane-bound form of immunoglobulin M. Immune Tg mice have 10,000-fold less specific Ab than wild-type mice and lack detectable ICs on FDCs. Nonetheless, primary immune responses and the GC reaction in these mice are robust, suggesting that ICs on FDCs do not play critical roles in immune response initiation and GC formation. Moreover, as indicated by the presence and pattern of somatic mutations, memory cell formation and selection appear normal in these IC-deficient GCs.

scholarly journals B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells

Blood ◽  
2009 ◽  
Vol 114 (24) ◽  
pp. 4989-4997 ◽  
Author(s):  
Marc Bajénoff ◽  
Ronald N. Germain
Keyword(s):  
Dendritic Cells ◽  
T Cell ◽  
B Cell ◽  
Soluble Antigen ◽  
Antigen Delivery ◽  
Follicular Dendritic Cells ◽  
Conduit System ◽  
Follicular Dendritic ◽  
Conduit Network ◽  
Cell Zone

Abstract Afferent lymph is transported throughout lymph nodes (LNs) by the conduit system. Whereas this conduit network is dense in the T-cell zone, it is sparse in B-cell follicles. In this study, we show that this differential organization emerges during lymph node development. Neonatal LNs lack B follicles, but have a developed T-cell zone and a dense conduit network. As new T and B cells enter the developing LN, the conduit network density is maintained in the T, but not the B zone, leading to a profound remodeling of the follicular network that nevertheless maintains its connectivity. In adults, the residual follicular conduits transport soluble antigen to deep regions, where follicular dendritic cells are abundant and appear to replace the fibroblastic reticular cells that enwrap conduits in the T zone. This strategic location correlates with the capacity of the follicular dendritic cells to capture antigen even in the absence of antigen-specific antibodies. Together, these results describe how the stromal organization of the T and B regions of LNs diverges during development, giving rise to distinct antigen transport and delivery modes in the 2 compartments.

scholarly journals Endocytosis and recycling of immune complexes by follicular dendritic cells enhances B cell binding and activation

2013 ◽  
Vol 4 ◽  
Author(s):  
Heesters Balthasar ◽  
Chatterjee Priyadarshini ◽  
Kim Young-A ◽  
Gonzalez Santiago ◽  
Kuligowski Michael ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
B Cell ◽  
Immune Complexes ◽  
Follicular Dendritic Cells ◽  
Cell Binding ◽  
Follicular Dendritic

Access to Follicular Dendritic Cells Is a Pivotal Step in Murine Chronic Lymphocytic Leukemia B-cell Activation and Proliferation

2014 ◽  
Vol 4 (12) ◽  
pp. 1448-1465 ◽  
Author(s):  
Kristina Heinig ◽  
Marcel Gätjen ◽  
Michael Grau ◽  
Vanessa Stache ◽  
Ioannis Anagnostopoulos ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Chronic Lymphocytic Leukemia ◽  
B Cell ◽  
Cell Activation ◽  
Lymphocytic Leukemia ◽  
Follicular Dendritic Cells ◽  
B Cell Activation ◽  
Follicular Dendritic
Sign in / Sign up

Export Citation Format

Share Document