scholarly journals Soluble CD14 is essential for lipopolysaccharide-dependent activation of human intestinal mast cells from macroscopically normal as well as Crohn's disease tissue

Immunology ◽  
2014 ◽  
Vol 143 (2) ◽  
pp. 174-183 ◽  
Author(s):  
Sibylle A. Brenner ◽  
Steffi Zacheja ◽  
Michael Schäffer ◽  
Katharina Feilhauer ◽  
Stephan C. Bischoff ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease ◽  
Soluble Cd14

Mast Cells and Headache in Crohn's Disease

1978 ◽  
Vol 18 (1) ◽  
pp. 40-43 ◽  
Author(s):  
Ruth Atkinson ◽  
Otto Appenzeller
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease

Macrophages and mast cells in focal active gastritis and helicobacter gastritis of Crohn's disease

1998 ◽  
Vol 114 ◽  
pp. A980
Author(s):  
H. Furusu ◽  
K. Murase ◽  
Y. Mizuta ◽  
H. Isomoto ◽  
Y. Nishida ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease

scholarly journals P430 Prognostic significance of neuropeptide expression in ileal neural plexuses in Crohn’s disease. A retrospective study

2021 ◽  
Vol 15 (Supplement_1) ◽  
pp. S433-S433
Author(s):  
A Gklavas ◽  
D Tiniakos ◽  
D Karandrea ◽  
G Karamanolis ◽  
G Bamias ◽  
...  
Keyword(s):  
Risk Factors ◽  
Crohn’S Disease ◽  
Mast Cells ◽  
T Lymphocytes ◽  
Crohn's Disease ◽  
Resection Margin ◽  
Prognostic Significance ◽  
Intestinal Resection ◽  
Independent Risk Factors ◽  
Proximal Resection Margin

Abstract Background Intestinal resection in Crohn’s disease (CD) is not curative and the risk for postoperative recurrence (POR) remains high. Highlighting risk factors for POR is crucial for the postoperative management of CD patients. Myenteric plexitis is a well-established risk factors for POR. The primary purpose of this study was to evaluate the correlation of neuropeptide P (NPY)-, vasoactive intestinal peptide (VIP)- and substance P (SP)-ergic nerve density with the presence and severity of plexitis in myenteric and submucosal plexuses in the proximal resection margin. Secondary aims were to assess the value of abovementioned neuropeptides’ expression in predicting POR and to recognize additional risk factors. Methods We conducted a retrospective, single-center study on CD patients who underwent ileocolonic resection (ICR) between January 2010 and December 2016. Exclusion criteria were age <16 years, patients with missing or invalid data precluding analysis, the presence of a diverting ileostomy on enrollment and specimens inappropriate for the evaluation of histologic features of interest in the proximal resection margin. Demographic and clinical data were retrieved, and the incidence or endoscopic, clinical and surgical POR was recorded. The presence and severity of plexitis was evaluated by hematoxylin and eosin staining. Giemsa staining was used for the recognition of mast cells. Immunohistochemistry was used was used for the detection of T-lymphocytes and NPY-, VIP- and SP-ergic neurons. The expression of the above peptides was quantified using image analysis. Results Seventy-nine patients (44 males) with a median age of 35 years were included. The median follow-up was 71 months. Myenteric and submucosal plexitis were present in 83.5% and 73.4% of patients, respectively. No association was detected between the density of NPY, VIP and SP expression and the presence or severity of plexitis. Similarly, the number of the involved T-lymphocytes or mast cells was not correlated with the expression of these peptides. Univariate and multivariate Cox proportional regression analysis was performed for the detection of risk factors for POR. Smoking and moderate/severe myenteric plexitis were independent risk factors for endoscopic and clinical POR, whereas an involved ileal margin was recognized as a risk factor for clinical POR. Conclusion This study did not document a correlation between plexitis in proximal resection margin and the expression of specific neuropeptides. According to our findings, smoking, myenteric plexitis, and involved ileal margin are independent risk factors for POR.

scholarly journals Mast Cells and Histamine Release in Crohn's Disease.

1993 ◽  
Vol 40 (3) ◽  
pp. 93-99 ◽  
Author(s):  
YASUMI ARAKI ◽  
TERUO KAKEGAWA ◽  
FLEMMING STADIL
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease ◽  
Histamine Release

P121 MAST CELLS AND EOSINOPHILS IN GASTRODUODENUAL MUCOSA OF CHILDREN WITH CROHN’S DISEASE

2019 ◽  
Vol 25 (Supplement_1) ◽  
pp. S58-S58
Author(s):  
Meenal Singh ◽  
Vivekanand Singh ◽  
Craig Friesen
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease

scholarly journals Possible Involvement of Intracellular Calcium-Independent Phospholipase A2 in the Release of Secretory Phospholipases from Mast Cells—Increased Expression in Ileal Mast Cells of Crohn’s Disease

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 672
Author(s):  
Ulrika Christerson ◽  
Åsa V. Keita ◽  
Martin E. Winberg ◽  
Johan D. Söderholm ◽  
Christina Gustafson-Svärd
Keyword(s):  
Crohn’S Disease ◽  
Mast Cells ◽  
Crohn's Disease ◽  
Ex Vivo ◽  
Calcium Ionophore ◽  
Human Mast Cell ◽  
Phospholipases A2 ◽  
Bromoenol Lactone ◽  
Inflammatory Processes ◽  
Calcium Independent Phospholipase A2

Increased activity of secretory phospholipases A2 (sPLA2) type-II was previously observed in ileum of Crohn’s disease (CD). Our aims were to explore the involvement of calcium-independent (i)PLA2β in the release of sPLA2s from the human mast cell (MC) line (HMC-1) and investigate expressions of cytosolic (c)PLA2α, iPLA2β, sPLA2-IIA and sPLA2-V in MCs of CD ileum. The release of sPLA2 was investigated in HMC-1 by immunocytochemistry and ELISA. The expression intensities of PLA2s in mucosal MCs, and the proportion of PLA2-positive MCs, were investigated in normal ileum and in ileum from patients with CD by immunohistochemistry. The calcium ionophore-stimulated release of sPLA2-IIA and sPLA2-V from HMC-1 was reduced by the iPLA2-inhibitor bromoenol lactone. All four PLA2s were detectable in mucosal MCs, both in normal ileum and in CD, but the proportion of iPLA2β-containing mucosal MCs and the expression intensity of sPLA2-IIA was increased in CD. Results indicate that iPLA2β is involved in the secretion of sPLA2s from HMC-1, and suggest that iPLA2β-mediated release of sPLA2 from intestinal MCs may contribute to CD pathophysiology. Ex vivo studies on isolated mucosal mast cells are however needed to clarify the precise role of MC PLA2s in the inflammatory processes of CD.

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