scholarly journals P7: THE IDENTIFICATION OF POTENTIAL ALLERGENS IN EDIBLE CRICKET ( ACHETA DOMESTICUS ): A COMPARATIVE ANALYSIS OF EXTRACTION METHODS AND PRAWN ALLERGEN‐SPECIFIC ANTIBODY REACTIVITY

2021 ◽  
Vol 51 (S4) ◽  
pp. 6-6
Keyword(s):  
Comparative Analysis ◽  
Specific Antibody ◽  
Extraction Methods ◽  
Acheta Domesticus ◽  
Antibody Reactivity

scholarly journals COMPARATIVE ANALYSIS OF DIFFERENT CAD METHODS FOR EXTRACTION OF THE HEMT NOISE WAVE MODEL PARAMETERS

2017 ◽  
Vol 16 (2) ◽  
pp. 117
Author(s):  
Vladica Đorđević ◽  
Zlatica Marinković ◽  
Olivera Pronić-Rančić
Keyword(s):  
Comparative Analysis ◽  
Electron Mobility ◽  
Computer Aided Design ◽  
Wave Model ◽  
High Electron Mobility Transistor ◽  
Extraction Methods ◽  
Model Parameters ◽  
High Electron ◽  
High Electron Mobility ◽  
Noise Modeling

The noise wave model has appeared as a very appropriate model for the purpose of transistor noise modeling at microwave frequencies. The transistor noise wave model parameters are usually extracted from the measured transistor noise parameters by using time-consuming optimization procedures in microwave circuit simulators. Therefore, three different Computer-Aided Design methods that enable more efficient automatic determination of these parameters in the case of high electron-mobility transistors were developed. All of these extraction methods are based on different noise de-embedding procedures, which are described in detail within this paper. In order to validate the presented extraction methods, they were applied for the noise modeling of a specific GaAs high electron-mobility transistor. Finally, the obtained results were used for the comparative analysis of the presented extraction approaches in terms of accuracy, complexity and effectiveness.

scholarly journals High Concentrations of Angiopoietin-Like Protein 4 Detected in Serum from Patients with Rheumatoid Arthritis Can Be Explained by Non-Specific Antibody Reactivity

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0168922
Author(s):  
Elena Makoveichuk ◽  
Toralph Ruge ◽  
Solveig Nilsson ◽  
Anna Södergren ◽  
Gunilla Olivecrona
Keyword(s):  
Rheumatoid Arthritis ◽  
Specific Antibody ◽  
Antibody Reactivity ◽  
High Concentrations

Comparative analysis of phase extraction methods based on phase-stepping and shifting curve in grating interferometry

2010 ◽  
Vol 19 (4) ◽  
pp. 040701 ◽  
Author(s):  
Liu Xiao-Song ◽  
Li En-Rong ◽  
Zhu Pei-Ping ◽  
Liu Yi-Jin ◽  
Zhang Kai ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Extraction Methods ◽  
Phase Extraction ◽  
Phase Stepping

scholarly journals Comparison of Conventional and Sustainable Lipid Extraction Methods for the Production of Oil and Protein Isolate from Edible Insect Meal

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 572 ◽  
Author(s):  
Laroche ◽  
Perreault ◽  
Marciniak ◽  
Gravel ◽  
Chamberland ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Protein Extraction ◽  
Lipid Extraction ◽  
Tenebrio Molitor ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Acheta Domesticus ◽  
Human Consumption ◽  
Alternative Source ◽  
Edible Insect

Edible insects represent an interesting alternative source of protein for human consumption but the main hurdle facing the edible insect sector is low consumer acceptance. However, increased acceptance is anticipated when insects are incorporated as a processed ingredient, such as protein-rich powder, rather than presented whole. To produce edible insect fractions with high protein content, a defatting step is necessary. This study investigated the effects of six defatting methods (conventional solvents, three-phase partitioning, and supercritical CO2) on lipid extraction yield, fatty profiles, and protein extraction and purification of house cricket (Acheta domesticus) and mealworm (Tenebrio molitor) meals. Ethanol increased the lipid extraction yield (22.7%–28.8%), irrespective of the insect meal used or the extraction method applied. Supercritical CO2 gave similar lipid extraction yields as conventional methods for Tenebrio molitor (T. molitor) (22.1%) but was less efficient for Acheta domesticus (A. domesticus) (11.9%). The protein extraction yield ranged from 12.4% to 38.9% for A. domesticus, and from 11.9% to 39.3% for T. molitor, whereas purification rates ranged from 58.3% to 78.5% for A. domesticus and from 48.7% to 75.4% for T. molitor.

scholarly journals Impaired Gamma Interferon Responses against Parvovirus B19 by Recently Infected Children

2000 ◽  
Vol 74 (21) ◽  
pp. 9903-9910 ◽  
Author(s):  
Amanda Corcoran ◽  
Sean Doyle ◽  
David Waldron ◽  
Alfred Nicholson ◽  
Bernard P. Mahon
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Responses ◽  
Specific Antibody ◽  
Parvovirus B19 ◽  
Gamma Interferon ◽  
Capsid Proteins ◽  
Exact Nature ◽  
Unique Region ◽  
Antibody Reactivity ◽  
Ifn Γ

ABSTRACT Parvovirus B19 is the causative agent of “fifth disease” of childhood. It has been implicated in a variety of conditions, including unsuccessful pregnancy and rheumatoid arthritis, and is a potential contaminant of blood products. There has been little study of immunity to parvovirus B19, and the exact nature of the protective humoral and cell-mediated immune response is unclear. Immune responses to purified virus capsid proteins, VP1 and VP2, were examined from a cohort of recently infected children and compared with responses from long-term convalescent volunteers. The results demonstrate that antibody reactivity is primarily maintained against conformational epitopes in VP1 and VP2. The unique region of VP1 appears to be a major target for cell-mediated immune responses, particularly in recently infected individuals. We confirm that antibody reactivity against linear epitopes of VP2 is lost shortly after infection but find no evidence of the proposed phenotypic switch in either the subclass of parvovirus B19-specific antibody or the pattern of cytokine production by antigen-specific T cells. The dominant subclass of specific antibody detected from both children and adults was immunoglobulin G1. No evidence was found for interleukin 4 (IL-4) or IL-5 production by isolated lymphocytes from children or adults. In contrast, lymphocytes from convalescent adults produced a typical type 1 response associated with high levels of IL-2 and gamma interferon (IFN-γ). However, we observed a significant (P < 0.001) deficit in the production of IFN-γ in response to VP1 or VP2 from lymphocytes isolated from children. Taken together, these results imply that future parvovirus B19 vaccines designed for children will require the use of conformationally preserved capsid proteins incorporating Th1 driving adjuvants. Furthermore, these data suggest novel mechanisms whereby parvovirus B19 infection may contribute to rheumatoid arthritis and unsuccessful pregnancy.

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