scholarly journals Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella

2019 ◽  
Vol 29 (2) ◽  
pp. 231-240
Author(s):  
T. Harvey‐Samuel ◽  
V. C. Norman ◽  
R. Carter ◽  
E. Lovett ◽  
L. Alphey
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Identification And Characterization

Molecular characterization and enzyme inhibition studies of NADP+- farnesol dehydrogenase from diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

2021 ◽  
Author(s):  
Anis-Nadyra Zifruddin ◽  
Khairunnisa-Atiqah Mohamad-Khalid ◽  
Saidi-Adha Suhaimi ◽  
Zeti-Azura Mohamed-Hussein ◽  
Maizom Hassan
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Maximum Activity ◽  
Binding Region ◽  
Relative Molecular Weight ◽  
Insect Reproduction ◽  
And Behavior ◽  
Inhibition Studies ◽  
Identification And Characterization

Abstract Juvenile hormone III (JH III) plays an important role in insect reproduction, development, and behavior. The second branch of JH III production includes oxidation of farnesol to farnesal by farnesol dehydrogenase. This study reported the identification and characterization of Plutella xylostella farnesol dehydrogenase (PxFoLDH). Our results showed that PxFoLDH belongs to the short-chain dehydrogenase/reductase superfamily, consisting of a single domain with a structurally conserved Rossman fold, an NAD(P) (H)—binding region and a structurally diverse C- terminal region. The purified enzyme displayed maximum activity at 55 °C with pH 9.5 and was stable in the temperature below 70 °C. PxFoLDH was determined to be a monomer with a relative molecular weight of 27 kDa and highly specific for trans, trans-farnesol and NADP+. Among analog inhibitors tested, farnesyl acetate was the most effective inhibitor with the lowest Ki value of 0.02 µM. Our findings showed this purified enzyme may represent as NADP+-farnesol dehydrogenase.

scholarly journals Genome-wide identification and characterization of putative lncRNAs in the diamondback moth, Plutella xylostella (L.)

Genomics ◽  
2018 ◽  
Vol 110 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Yue Wang ◽  
Tingting Xu ◽  
Weiyi He ◽  
Xiujing Shen ◽  
Qian Zhao ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Genome Wide ◽  
Identification And Characterization

Identification and characterization of the vasa gene in the diamondback moth, Plutella xylostella

2020 ◽  
Vol 122 ◽  
pp. 103371 ◽  
Author(s):  
Xuejiao Xu ◽  
Jie Yang ◽  
Tim Harvey-Samuel ◽  
Yuping Huang ◽  
Muhammad Asad ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Vasa Gene ◽  
Identification And Characterization

scholarly journals Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

2013 ◽  
Vol 14 (11) ◽  
pp. 22891-22905 ◽  
Author(s):  
Lixia Tian ◽  
Jiaqiang Yang ◽  
Wenjie Hou ◽  
Baoyun Xu ◽  
Wen Xie ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
P Glycoprotein
Sign in / Sign up

Export Citation Format

Share Document