Molecular characterization and enzyme inhibition studies of NADP+- farnesol dehydrogenase from diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

2021 ◽  
Author(s):  
Anis-Nadyra Zifruddin ◽  
Khairunnisa-Atiqah Mohamad-Khalid ◽  
Saidi-Adha Suhaimi ◽  
Zeti-Azura Mohamed-Hussein ◽  
Maizom Hassan
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Maximum Activity ◽  
Binding Region ◽  
Relative Molecular Weight ◽  
Insect Reproduction ◽  
And Behavior ◽  
Inhibition Studies ◽  
Identification And Characterization

Abstract Juvenile hormone III (JH III) plays an important role in insect reproduction, development, and behavior. The second branch of JH III production includes oxidation of farnesol to farnesal by farnesol dehydrogenase. This study reported the identification and characterization of Plutella xylostella farnesol dehydrogenase (PxFoLDH). Our results showed that PxFoLDH belongs to the short-chain dehydrogenase/reductase superfamily, consisting of a single domain with a structurally conserved Rossman fold, an NAD(P) (H)—binding region and a structurally diverse C- terminal region. The purified enzyme displayed maximum activity at 55 °C with pH 9.5 and was stable in the temperature below 70 °C. PxFoLDH was determined to be a monomer with a relative molecular weight of 27 kDa and highly specific for trans, trans-farnesol and NADP+. Among analog inhibitors tested, farnesyl acetate was the most effective inhibitor with the lowest Ki value of 0.02 µM. Our findings showed this purified enzyme may represent as NADP+-farnesol dehydrogenase.

scholarly journals Identification and characterization of a Masculinizer homologue in the diamondback moth, Plutella xylostella

2019 ◽  
Vol 29 (2) ◽  
pp. 231-240
Author(s):  
T. Harvey‐Samuel ◽  
V. C. Norman ◽  
R. Carter ◽  
E. Lovett ◽  
L. Alphey
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Identification And Characterization

scholarly journals Genome-wide identification and characterization of putative lncRNAs in the diamondback moth, Plutella xylostella (L.)

Genomics ◽  
2018 ◽  
Vol 110 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Yue Wang ◽  
Tingting Xu ◽  
Weiyi He ◽  
Xiujing Shen ◽  
Qian Zhao ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Genome Wide ◽  
Identification And Characterization

Identification and characterization of the vasa gene in the diamondback moth, Plutella xylostella

2020 ◽  
Vol 122 ◽  
pp. 103371 ◽  
Author(s):  
Xuejiao Xu ◽  
Jie Yang ◽  
Tim Harvey-Samuel ◽  
Yuping Huang ◽  
Muhammad Asad ◽  
...  
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Vasa Gene ◽  
Identification And Characterization

Effects of rhizobacteria on the biology and behavior of Plutella xylostella (Lepidoptera: Plutellidae)

2017 ◽  
Vol 43 (2) ◽  
pp. 195
Author(s):  
Robson Thomaz Thuler ◽  
Fernando Henrique Iost Filho ◽  
Hamilton César De Oliveira Charlo ◽  
Sergio Antônio De Bortoli
Keyword(s):  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Insect Behavior ◽  
Control Treatment ◽  
Pupal Weight ◽  
Larval Feeding ◽  
Direct Effects ◽  
Plant Feeding ◽  
Kluyvera Ascorbata ◽  
And Behavior

Plant induced resistance is a tool for integrated pest management, aimed at increasing plant defense against stress, which is compatible with other techniques. Rhizobacteria act in the plant through metabolic changes and may have direct effects on plant-feeding insects. The objective of this study was to determine the effects of cabbage plants inoculated with rhizobacteria on the biology and behavior of diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Cabbage seeds inoculated with 12 rhizobacteria strains were sowed in polystyrene trays and later transplanted into the greenhouse. The cabbage plants with sufficient size to support stress were then infested with diamondback moth caterpillars. Later, healthy leaves suffering injuries were collected and taken to the laboratory to feed P. xylostella second instar caterpillars that were evaluated for larval and pupal viability and duration, pupal weight, and sex ratio. The reduction of leaf area was then calculated as a measure of the amount of larval feeding. Non-preference for feeding and oviposition assays were also performed, by comparing the control treatment and plants inoculated with different rhizobacterial strains. Plants inoculated with the strains EN4 of Kluyvera ascorbata and HPF14 of Bacillus thuringiensis negatively affected the biological characteristics of P. xylostella when such traits were evaluated together, without directly affecting the insect behavior.

scholarly journals Molecular Cloning and Characterization of a P-Glycoprotein from the Diamondback Moth, Plutella xylostella (Lepidoptera: Plutellidae)

2013 ◽  
Vol 14 (11) ◽  
pp. 22891-22905 ◽  
Author(s):  
Lixia Tian ◽  
Jiaqiang Yang ◽  
Wenjie Hou ◽  
Baoyun Xu ◽  
Wen Xie ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
P Glycoprotein
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