scholarly journals The distribution of total vitamin B12, holotranscobalamin, and the active vitamin B12 fraction in the first 5 weeks postpartum

2017 ◽  
Vol 40 (1) ◽  
pp. 72-76 ◽  
Author(s):  
D. A. A. van der Woude ◽  
J. M. A. Pijnenborg ◽  
J. de Vries ◽  
E. M. van Wijk
Keyword(s):  
Vitamin B12 ◽  
Active Vitamin ◽  
Total Vitamin

scholarly journals Feeding dried purple laver (nori) to vitamin B12-deficient rats significantly improves vitamin B12 status

2001 ◽  
Vol 85 (6) ◽  
pp. 699-703 ◽  
Author(s):  
Shigeo Takenaka ◽  
Sumi Sugiyama ◽  
Shuhei Ebara ◽  
Emi Miyamoto ◽  
Katsuo Abe ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Methylmalonic Acid ◽  
Intrinsic Factor ◽  
Porphyra Yezoensis ◽  
Dry Weight ◽  
Biologically Active ◽  
Vitamin B ◽  
Acid Excretion ◽  
Active Vitamin ◽  
Total Vitamin

To clarify the bioavailability of vitamin B12 in lyophylized purple laver (nori; Porphyra yezoensis), total vitamin B12 and vitamin B12 analogue contents in the laver were determined, and the effects of feeding the laver to vitamin B12-deficient rats were investigated. The amount of total vitamin B12 in the dried purple laver was estimated to be 54.5 and 58.6 (SE 5.3 and 7.5 respectively) ΜG/100 g dry weight by lactobacillus bioassay and chemiluminescent assay with hog intrinsic factor respectively. the purple laver contained five types of biologically active vitamin b12 compounds (cyano-, hydroxo-, sulfito-, adenosyl- and methylcobalamin), in which the vitamin b12 coezymes (adenosyl- and methylcobalamin) comprised about 60 % of the total vitamin b12. when 9-week-old vitamin b12-deficient rats, which excreted substantial amounts of methylmalonic acid (71.7(se 20.2) μmol/d) in urine, were fed the diet supplemented with dried purple laver (10 μg/kg diet) for 20 d, urinary methylmalonic acid excretion (as an index of vitamin B12 deficiency) became undetectable and hepatic vitamin B12 (especially adenosylcobalamin) levels were significantly increased. These results indicate that vitamin B12 in dried purple laver is bioavailable to rats.

scholarly journals In situproduction of active vitamin B12 in cereal matrices usingPropionibacterium freudenreichii

2017 ◽  
Vol 6 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Bhawani Chamlagain ◽  
Tessa A. Sugito ◽  
Paulina Deptula ◽  
Minnamari Edelmann ◽  
Susanna Kariluoto ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Active Vitamin

THE CONSTITUENTS OF COD LIVER WITH VITAMIN B12 ACTIVITY FOR LACTOBACILLUS LEICHMANNII

1956 ◽  
Vol 34 (2) ◽  
pp. 191-196
Author(s):  
Beryl Truscott ◽  
P. L. Hoogland
Keyword(s):  
Ion Exchange ◽  
Vitamin B12 ◽  
Microbiological Assay ◽  
Countercurrent Distribution ◽  
Vitamin B ◽  
Partition Chromatography ◽  
Total Vitamin ◽  
Beef Liver ◽  
Fractionation Procedure ◽  
Lactobacillus Leichmannii

The identity of the compounds that together form the total microbiological vitamin B12 activity of cod-liver residue was determined by a fractionation procedure involving ion exchange, countercurrent distribution, partition chromatography, and microbiological assay. The presence of cyanocobalamin, hydroxocobalamin, and the desoxyribosides of thymine, uracil, hypoxanthine, xanthine, and guanine was demonstrated. The total vitamin B12 activity, as determined by microbiological assay with Lactobacillus leichmannii, and its distribution in cod-liver residue, were very similar to those of beef liver. Cod-liver residues may be used to replace beef liver in vitamin B12 preparations.

scholarly journals Changes in Vitamin E and β-Carotene Contents in Various Edible Cassava Leaves (Manihot esculenta Crantz) of Different Ages across Multiple Seasons

2020 ◽  
Vol 2020 ◽  
pp. 1-8
Author(s):  
Alphonse Laya ◽  
Benoît B. Koubala
Keyword(s):  
Vitamin E ◽  
Biologically Active ◽  
Leafy Vegetables ◽  
Active Vitamin ◽  
Total Vitamin ◽  
Manihot Esculenta Crantz ◽  
Liquid Chromatograph ◽  
Cassava Leaves ◽  
Significant Difference ◽  
Different Ages

Vitamin E and carotenoids belong to a group of bioactive compounds that have an important effect on human health. The present study aims to investigate for the first time the concentration of vitamin E, α-, γ-, and δ-tocopherol, and β-carotene in edible cassava leaves during different ages. The analysis was performed using colorimetry and high-performance liquid chromatograph (HPLC) methods. A significant difference was found among α-, γ-, and δ-tocopherol concentrations in leaves; γ- and α-tocopherol isomer contents were the predominant in amount, respectively. Among the leaves, AD variety harvested at 6 months after planting (MAP) was the highest in total vitamin E (222 μgα-TE/g). However, the highest γ-tocopherol content (2782 μg/100g) and the content of biologically active vitamin E (1244 μg/100g) were found in EN variety at 6MAP, whereas the highest value (42 μg/g) of β-carotene was found in AD variety at 12MAP. Total vitamin E and tocopherol isomers composition varied among varieties and seasons as did β-carotene. The effects of varieties and harvest ages on the biosynthesis regulation of these compounds were confirmed by principal component analysis (F1x F2: 74.34%). Edible cassava leaves can be considered as sources of vitamin E for natural dietary antioxidant during different ages, and the best time to harvest EN and AD varieties is at 6MAP on the basis of α-tocopherol equivalent or content of biologically active vitamin E content, and EN and AD varieties performed the overall best. Thus, the edible cassava leaves are among the leafy vegetables that could be helpful to suggest as a part of daily meal for health benefits and pharmaceutical purposes.

scholarly journals Estimating the Serum Folate Concentration Associated With the Red Blood Cell Folate Threshold for Optimal Neural Tube Defects Prevention: A Population Based Biomarker Survey

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 644-644
Author(s):  
Amy Fothergill ◽  
Charles Rose ◽  
Krista Crider ◽  
Beena Bose ◽  
Heather Guetterman ◽  
...  
Keyword(s):  
Blood Cell ◽  
Vitamin B12 ◽  
Neural Tube ◽  
Red Blood Cell ◽  
Vitamin B12 Deficiency ◽  
Population Based ◽  
World Health ◽  
Serum Folate ◽  
Total Vitamin ◽  
B12 Deficiency

Abstract Objectives To estimate the serum folate insufficiency threshold (sf-IT) corresponding to the red blood cell (RBC) folate insufficiency threshold for optimal neural tube defect (NTD) prevention. Methods Participants were 977 women of reproductive age (WRA; 15–40y; not pregnant or lactating) from a population-based biomarker survey in Southern India. Venous blood samples were collected at enrollment. Plasma, serum, and red blood cells were centrifuged, processed, and stored < -80°C until batch analysis. Total vitamin B12 concentrations were measured via chemiluminescence; RBC and serum folate concentrations were measured using the World Health Organization-recommended microbiological assay. Vitamin B12 deficiency was defined as total vitamin B12 < 148 pmol/L. Folate insufficiency was defined as RBC folate < 748 nmol/L, the recommended calibrator-adjusted equivalent of the threshold for population optimal NTD prevention. A previously developed Bayesian model and the RBC and serum folate distributions in this population were used to estimate the sf-IT corresponding to the RBC folate insufficiency threshold for optimal NTD prevention, overall and by age, body mass index (BMI) category, HbA1c, anemia, and vitamin B12 status. Results The overall estimated median sf-IT was 37.8 nmol/L (95% credible interval [33.8–43.3]). This threshold was lower in overweight WRA (BMI: ≥25.0 kg/m2: 32.0 nmol/L [27.3–40.2] vs. BMI < 25.0 kg/m2: 36.2 nmol/L [32.2–43.3]), and varied by age (< 25y: 61.3 nmol/L [44.3–111.8]; 25 to 35y: 35.7 nmol/L [30.8–43.5]; ≥35y: 30.8 nmol/L [26.9–37.2]). The sf-IT was lower in anemic WRA (32.9 nmol/L [28.5–40.1]) compared to non-anemic WRA (42.0 nmol/L [36.1–51.3]), and lower in WRA with elevated HbA1c (≥5.7% to < 6.5: 32.4 nmol/L [27.3–41.6]; ≥6.5%: 20.9 nmol/L [17.8–25.6]) vs. WRA with HbA1c < 5.7% (43.8 nmol/L, [37.5–53.7]). The median sf-IT was higher in WRA with vitamin B12 deficiency (72.1 nmol/L [52.0–126.0]), compared to women who were not vitamin B12 deficient (28.1 nmol/L [25.6–31.5]). Conclusions The estimated sf-IT is dependent on anemia, elevated HbA1c, BMI, age, and vitamin B12 status. Funding Sources Centers for Disease Control and Prevention; AF was supported by the National Institutes of Health #5 T32 HD087137.

scholarly journals Cross-sectional Association Between Vitamin B12 Status and Probable Postpartum Depression in Indian Women

2020 ◽  
Author(s):  
Pooja Dhiman ◽  
Raji R Pillai ◽  
Anand B Wilson ◽  
Nancy Premkumar ◽  
Balaji Bharadwaj ◽  
...  
Keyword(s):  
Depressive Symptoms ◽  
Postpartum Depression ◽  
Vitamin B12 ◽  
Depression Scale ◽  
Biologically Active ◽  
Total Vitamin ◽  
Active Molecule ◽  
Cross Sectional ◽  
Indian Women ◽  
Probable Depression

Abstract BackgroundVitamin B12 is an essential micronutrient for neurological function, as it leads to the regeneration of methionine from homocysteine, which is precursor of biologically active molecule SAM. Pregnancy is a state of increased demand and delayed postpartum repletion of nutrients may predispose women to depression. MethodsWomen attending postpartum clinic at 6 weeks post-delivery were screened for the presence of depressive symptoms using Edinburg postpartum depression scale (EPDS). Based on a cut-off of 10, two groups were taken- women with probable depression with EPDS score>10 (n=217) and women without probable depression with EPDS score<10(n=217). Plasma total vitamin B12, holotranscobalamin, homocysteine, MMA, 5-methyl THF, SAM and serotonin levels were estimated using commercially available ELISA kits. Combined B12(cB12) score was calculated and analysis of resulting data was done using SPSS ver20.0. ResultsTotal vitamin B12 was significantly lower (p=0.001) and MMA (p=0.002) and 5-methyl THF(p<0.001) levels were higher in women with probable depression than women without probable depression. Combined B12 index was also lower in women with probable PPD (p=0.001). Women in the lowest vitamin B12 quartile had 4.53 times higher likelihood of probable postpartum depression (p < 0.001). Decreasing vitamin B12 (OR = 0.394; 95% CI: 0.189-0.822) and cB12 (OR=0.293; 95% CI:0182-0.470) and increasing MMA (OR = 2.14; 95% CI: 1.63-2.83) and 5-methyl THF levels (OR = 3.29; 95% CI: 1.59-6.83) were significantly associated with the risk of PPD.ConclusionLow vitamin B12 may contribute to depressive symptoms in vulnerable postpartum period.

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