The association of −330 interleukin-2 gene polymorphism and HLA-DR15 allele in Iranian patients with multiple sclerosis

2014 ◽  
Vol 41 (4) ◽  
pp. 330-334 ◽  
Author(s):  
A. Sayad
Keyword(s):  
Multiple Sclerosis ◽  
Gene Polymorphism ◽  
Interleukin 2 ◽  
Iranian Patients

scholarly journals Neurofilament heavy chain gene polymorphism and risk of multiple sclerosis

2020 ◽  
Vol 22 (4) ◽  
pp. 155-158
Author(s):  
Elham Ghorbani Jazar ◽  
Seyedeh Parisa Chavoshi Tarzjani ◽  
Zahra Sadeghi ◽  
Shekoofe Alaie ◽  
Seyed Abolhassan Shahzadeh Fazeli
Keyword(s):  
Multiple Sclerosis ◽  
Gene Polymorphism ◽  
Heavy Chain ◽  
Sanger Sequencing ◽  
Chain Gene ◽  
Heavy Chain Gene ◽  
Chain Reaction ◽  
The Central Nervous System ◽  
Polymerase Chain ◽  
Iranian Patients

Multiple sclerosis (MS) is a chronic disease characterized by degeneration of the central nervous system (CNS). High levels of Neurofilament heavy chain (NEFH) in cerebrospinal fluid (CSF) is associated with MS. 40 MS patients and 40 controls genotyped by polymerase chain reaction (PCR) and Sanger sequencing. Genotypic and allelic distributions were compared between cases and controls. Fisher test was used to estimate the risk of MS associated with genotypes. We showed that NEFH, 1084-244G>A gene polymorphism, has no significant association with the susceptibility or severity of MS in Iranian patients (P = 0.737). Further prospective studies are required for confirmation.

Association between single nucleotide polymorphisms rs12722489 and multiple sclerosis in Iranian patients with multiple sclerosis

2020 ◽  
Author(s):  
Habib Ahmadi ◽  
Vahid Reza Yassaee ◽  
Reza Mirfakhraie ◽  
Feyzollah Hashemi-Gorji
Keyword(s):  
Multiple Sclerosis ◽  
Single Nucleotide Polymorphisms ◽  
Interleukin 2 ◽  
European Population ◽  
Control Group ◽  
Amplification Refractory Mutation System ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Mutation System ◽  
Iranian Patients

Abstract Background: Multiple sclerosis (MS) is a complex incurable neurodegenerative disease featuring demyelination of neurons, resulting in impairment of neuron impulses. Recently, an association of two single nucleotide polymorphisms (SNP) (rs2104286 and rs12722489) in interleukin 2 receptor subunit alpha (IL2RA) gene was found to be a risk factor of MS in white European population. Therefore, we performed a study to investigate the contribution of these two intronic variations in Iranian patients with MS. Methods: We determined the genotypes of rs2104286 and rs12722489 in patients with MS (n = 100) and in the control group (n = 111). The SNPs were genotyped using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR) for both of SNPs. Statistical analysis was performed by SPSS software. Also, odds ratios (ORs) and 95% confidence interval (CI) were calculated. Results: Logistic regression revealed that various genotypes of rs12722489, regarding sex-adjusted effect, yielded meaningful association with MS risk in Iranian patients (OR = 2.67, 95% CI: 1.03-6.90). However, no association was obtained for rs2104286 and rs12722489 with MS. Conclusion: The results confirmed partially the reports in white European population performed recently. However, further investigation in larger scale is necessary to validate our study.

scholarly journals The Association of −330 Interleukin-2 Gene Polymorphism with Its Plasma Concentration in Iranian Multiple Sclerosis Patients

Scientifica ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-4 ◽  
Author(s):  
Arezou Sayad ◽  
Abolfazl Movafagh
Keyword(s):  
Multiple Sclerosis ◽  
Plasma Concentration ◽  
Gene Polymorphism ◽  
Plasma Levels ◽  
Demyelinating Disease ◽  
Interleukin 2 ◽  
Control Group ◽  
Normal Individuals ◽  
The Central Nervous System ◽  
Multiple Sclerosis Patients

Multiple sclerosis (MS) is a chronic neuroinflammatory demyelinating disease of the central nervous system. The cytokine genes are involved in autoimmune diseases such as MS. In this study, we report the influence of −330 interleukin-2 (IL2) gene polymorphism on its plasma levels in a group of Iranian MS patients. In this study 100 MS patients and 100 ethnically, age, and sex matched healthy controls were selected from Medical Genetics Department of Sarem Women Hospital. Blood samples of all individuals were collected in EDTA tubes. The restriction fragment length polymorphism PCR (RFLP) method was applied to determine various alleles and genotypes in these individuals. Plasma concentration of IL2 was measured in all the samples using human IL2 kit. The frequency of −330 T/T IL2 genotype was higher in MS patients compared to normal individuals. Accordingly, the plasma levels of IL2 were significantly higher (P<0.0001) in patients when compared to the control group. In conclusion, in case of MS patients the −330 T/T IL2 genotype is associated with higher plasma levels of IL2.

scholarly journals Lack of Association of Interleukin-8 Gene Polymorphism with Helicobacter pylori-Induced Gastritis in Iranian Patients

2008 ◽  
Vol 12 ◽  
pp. e213
Author(s):  
S.H. Farshad ◽  
M. Rasouli ◽  
A. Jamshidzadeh ◽  
A. Hosseinkhani ◽  
A. Japoni ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gene Polymorphism ◽  
Interleukin 8 ◽  
Iranian Patients
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